Dynamic bacterial and viral response to an algal bloom at subzero temperatures
New evidence suggests that cold‐loving (psychrophilic) bacteria may be a dynamic component of the episodic bloom events of high‐latitude ecosystems. Here we report the results of an unusually early springtime study of pelagic microbial activity in the coastal Alaskan Arctic. Heterotrophic bacteriopl...
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crwiley:10.4319/lo.2001.46.4.0790 2024-09-09T19:23:48+00:00 Dynamic bacterial and viral response to an algal bloom at subzero temperatures Yager, Patricia L. Connelly, Tara L. Mortazavi, Behzad Wommack, K. Eric Bano, Nasreen Bauer, James E. Opsahl, Stephen Hollibaugh, James T. 2001 http://dx.doi.org/10.4319/lo.2001.46.4.0790 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2001.46.4.0790 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2001.46.4.0790 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 46, issue 4, page 790-801 ISSN 0024-3590 1939-5590 journal-article 2001 crwiley https://doi.org/10.4319/lo.2001.46.4.0790 2024-07-23T04:09:41Z New evidence suggests that cold‐loving (psychrophilic) bacteria may be a dynamic component of the episodic bloom events of high‐latitude ecosystems. Here we report the results of an unusually early springtime study of pelagic microbial activity in the coastal Alaskan Arctic. Heterotrophic bacterioplankton clearly responded to an algal bloom by doubling cell size, increasing the fraction of actively respiring cells (up to an unprecedented 84% metabolically active using redox dye CTC), shifting substrate‐uptake capabilities from kinetic parameters better adapted to lower substrate concentrations to those more suited for higher concentrations, and more than doubling cell abundance. Community composition (determined by polymerase chain reaction/DGGE and nucleotide sequence analysis) also shifted over the bloom. Results support, for the first time with modern molecular methods, previous culture‐based observations of bacterial community succession during Arctic algal blooms and confirm that previously observed variability in pelagic microbial activity can be linked to changes in community structure. During early bloom stages, virioplankton and bacterial abundance were comparable, suggesting that mortality due to phage infection was low at that time. The virus‐to‐bacteria ratio (VBR) increased 10‐fold at the height of the bloom, however, suggesting an increased potential for bacterioplankton mortality resulting from viral infection. The peak in VBR coincided with observed shifts in both microbial activity and community structure. These early‐season data suggest that substrate and virioplankton interactions may control the active microbial carbon cycling of this region. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Limnology and Oceanography 46 4 790 801 |
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Wiley Online Library |
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crwiley |
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English |
description |
New evidence suggests that cold‐loving (psychrophilic) bacteria may be a dynamic component of the episodic bloom events of high‐latitude ecosystems. Here we report the results of an unusually early springtime study of pelagic microbial activity in the coastal Alaskan Arctic. Heterotrophic bacterioplankton clearly responded to an algal bloom by doubling cell size, increasing the fraction of actively respiring cells (up to an unprecedented 84% metabolically active using redox dye CTC), shifting substrate‐uptake capabilities from kinetic parameters better adapted to lower substrate concentrations to those more suited for higher concentrations, and more than doubling cell abundance. Community composition (determined by polymerase chain reaction/DGGE and nucleotide sequence analysis) also shifted over the bloom. Results support, for the first time with modern molecular methods, previous culture‐based observations of bacterial community succession during Arctic algal blooms and confirm that previously observed variability in pelagic microbial activity can be linked to changes in community structure. During early bloom stages, virioplankton and bacterial abundance were comparable, suggesting that mortality due to phage infection was low at that time. The virus‐to‐bacteria ratio (VBR) increased 10‐fold at the height of the bloom, however, suggesting an increased potential for bacterioplankton mortality resulting from viral infection. The peak in VBR coincided with observed shifts in both microbial activity and community structure. These early‐season data suggest that substrate and virioplankton interactions may control the active microbial carbon cycling of this region. |
format |
Article in Journal/Newspaper |
author |
Yager, Patricia L. Connelly, Tara L. Mortazavi, Behzad Wommack, K. Eric Bano, Nasreen Bauer, James E. Opsahl, Stephen Hollibaugh, James T. |
spellingShingle |
Yager, Patricia L. Connelly, Tara L. Mortazavi, Behzad Wommack, K. Eric Bano, Nasreen Bauer, James E. Opsahl, Stephen Hollibaugh, James T. Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
author_facet |
Yager, Patricia L. Connelly, Tara L. Mortazavi, Behzad Wommack, K. Eric Bano, Nasreen Bauer, James E. Opsahl, Stephen Hollibaugh, James T. |
author_sort |
Yager, Patricia L. |
title |
Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
title_short |
Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
title_full |
Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
title_fullStr |
Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
title_full_unstemmed |
Dynamic bacterial and viral response to an algal bloom at subzero temperatures |
title_sort |
dynamic bacterial and viral response to an algal bloom at subzero temperatures |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.4319/lo.2001.46.4.0790 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2001.46.4.0790 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2001.46.4.0790 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Limnology and Oceanography volume 46, issue 4, page 790-801 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.2001.46.4.0790 |
container_title |
Limnology and Oceanography |
container_volume |
46 |
container_issue |
4 |
container_start_page |
790 |
op_container_end_page |
801 |
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1809893769001041920 |