Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments
Pulsed field gel electrophoresis (PFGE) was used to determine the size distributions of virus‐like DNA in seawater from diverse environments (Arctic Ocean, Ross Sea, coastal Pacific Ocean, and northern Adriatic Sea). Changes in DNA banding patterns indicated that shifts in the viral assemblage compo...
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crwiley:10.4319/lo.2000.45.8.1697 2024-06-23T07:50:31+00:00 Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments Steward, Grieg F. Montiel, Janine L. Azam, Farooq 2000 http://dx.doi.org/10.4319/lo.2000.45.8.1697 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2000.45.8.1697 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2000.45.8.1697 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 45, issue 8, page 1697-1706 ISSN 0024-3590 1939-5590 journal-article 2000 crwiley https://doi.org/10.4319/lo.2000.45.8.1697 2024-06-06T04:21:47Z Pulsed field gel electrophoresis (PFGE) was used to determine the size distributions of virus‐like DNA in seawater from diverse environments (Arctic Ocean, Ross Sea, coastal Pacific Ocean, and northern Adriatic Sea). Changes in DNA banding patterns indicated that shifts in the viral assemblage composition occurred on the order of #2 d during an intense dinoflagellate bloom in coastal Pacific waters. Different DNA banding patterns from diverse locations also indicated spatial variability in composition, but all of the samples analyzed had similar features. Size frequency distributions for virus‐like genomes (VLGs) were multimodal, with major peaks occurring around 31–36 kilobases (kb) and 58–63 kb. The smallest discrete band resolved was 26 kb, the largest was >200 kb, and the overall mean VLG size was 50 ± 4 kb (mean ± SD, n = 30). On average in surface seawater, >90% of the VLGs occurred in the 26–69 kb size range, and at least half were between 28 and 45 kb. This first extensive survey of viral genome sizes in seawater indicates that most marine viruses have physical properties similar to other known viruses. The distributions revealed that the vast majority of the detected VLGs had sizes typical of bacteriophages, whereas only a few percent were in the size range of known algal viruses. Article in Journal/Newspaper Arctic Arctic Ocean Ross Sea Wiley Online Library Arctic Arctic Ocean Pacific Ross Sea Limnology and Oceanography 45 8 1697 1706 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Pulsed field gel electrophoresis (PFGE) was used to determine the size distributions of virus‐like DNA in seawater from diverse environments (Arctic Ocean, Ross Sea, coastal Pacific Ocean, and northern Adriatic Sea). Changes in DNA banding patterns indicated that shifts in the viral assemblage composition occurred on the order of #2 d during an intense dinoflagellate bloom in coastal Pacific waters. Different DNA banding patterns from diverse locations also indicated spatial variability in composition, but all of the samples analyzed had similar features. Size frequency distributions for virus‐like genomes (VLGs) were multimodal, with major peaks occurring around 31–36 kilobases (kb) and 58–63 kb. The smallest discrete band resolved was 26 kb, the largest was >200 kb, and the overall mean VLG size was 50 ± 4 kb (mean ± SD, n = 30). On average in surface seawater, >90% of the VLGs occurred in the 26–69 kb size range, and at least half were between 28 and 45 kb. This first extensive survey of viral genome sizes in seawater indicates that most marine viruses have physical properties similar to other known viruses. The distributions revealed that the vast majority of the detected VLGs had sizes typical of bacteriophages, whereas only a few percent were in the size range of known algal viruses. |
format |
Article in Journal/Newspaper |
author |
Steward, Grieg F. Montiel, Janine L. Azam, Farooq |
spellingShingle |
Steward, Grieg F. Montiel, Janine L. Azam, Farooq Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
author_facet |
Steward, Grieg F. Montiel, Janine L. Azam, Farooq |
author_sort |
Steward, Grieg F. |
title |
Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
title_short |
Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
title_full |
Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
title_fullStr |
Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
title_full_unstemmed |
Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
title_sort |
genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.4319/lo.2000.45.8.1697 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2000.45.8.1697 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2000.45.8.1697 |
geographic |
Arctic Arctic Ocean Pacific Ross Sea |
geographic_facet |
Arctic Arctic Ocean Pacific Ross Sea |
genre |
Arctic Arctic Ocean Ross Sea |
genre_facet |
Arctic Arctic Ocean Ross Sea |
op_source |
Limnology and Oceanography volume 45, issue 8, page 1697-1706 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.2000.45.8.1697 |
container_title |
Limnology and Oceanography |
container_volume |
45 |
container_issue |
8 |
container_start_page |
1697 |
op_container_end_page |
1706 |
_version_ |
1802641420701401088 |