Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach
To test the hypothesized inhibition of high‐latitude marine bacteria living at lower temperatures when organic matter is scarce, the effects of temperature and organic substrate concentration on pelagic microbial heterotrophy were investigated in the perennially cold surface waters of a summertime a...
Published in: | Limnology and Oceanography |
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crwiley:10.4319/lo.1999.44.8.1882 2023-12-03T10:17:05+01:00 Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach Yager, Patricia L. Deming, Jody W. 1999 http://dx.doi.org/10.4319/lo.1999.44.8.1882 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1999.44.8.1882 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1999.44.8.1882 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 44, issue 8, page 1882-1893 ISSN 0024-3590 1939-5590 Aquatic Science Oceanography journal-article 1999 crwiley https://doi.org/10.4319/lo.1999.44.8.1882 2023-11-09T14:03:40Z To test the hypothesized inhibition of high‐latitude marine bacteria living at lower temperatures when organic matter is scarce, the effects of temperature and organic substrate concentration on pelagic microbial heterotrophy were investigated in the perennially cold surface waters of a summertime arctic polynya. Utilization (incorporation plus respiration) of radiolabeled amino acids was measured as a function of increasing added substrate concentration at in situ (subzero) temperature and under short‐term warming. Results analyzed using analysis of variance (ANOVA) showed that increased substrate concentration had a significant effect on utilization rates at all stations, suggesting that communities were always living well below saturating levels of substrate. About half of the communities sampled revealed a significant temperature response using ANOVA; interactions between temperature and substrate concentration were detected only rarely. Kinetic parameters, used to link microbial activity and substrate utilization with temperature sensitivity, exhibited mixed responses to short‐term warming. Maximum specific utilization rates showed the greater temperature sensitivity, with Q 10 values ranging from 0.25 to 13. Specific affinities responded to temperature significantly at only about half of the stations in the polynya. Psychrophilic behaviors (e.g., highest specific affinities and oligotrophic capacities at lower incubation temperatures) were observed at stations most likely to be influenced by direct Arctic Ocean outflow. Complete agreement with the hypothesis of enhanced substrate requirement by bacteria living at subzero temperature was not found. An improved understanding of the diversity of cold‐tolerant and cold‐loving microorganisms is needed before generalizing or predicting the role of temperature in the cycling of organic matter at high latitudes. Article in Journal/Newspaper Arctic Arctic Ocean Wiley Online Library (via Crossref) Arctic Arctic Ocean Limnology and Oceanography 44 8 1882 1893 |
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Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Oceanography |
spellingShingle |
Aquatic Science Oceanography Yager, Patricia L. Deming, Jody W. Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
topic_facet |
Aquatic Science Oceanography |
description |
To test the hypothesized inhibition of high‐latitude marine bacteria living at lower temperatures when organic matter is scarce, the effects of temperature and organic substrate concentration on pelagic microbial heterotrophy were investigated in the perennially cold surface waters of a summertime arctic polynya. Utilization (incorporation plus respiration) of radiolabeled amino acids was measured as a function of increasing added substrate concentration at in situ (subzero) temperature and under short‐term warming. Results analyzed using analysis of variance (ANOVA) showed that increased substrate concentration had a significant effect on utilization rates at all stations, suggesting that communities were always living well below saturating levels of substrate. About half of the communities sampled revealed a significant temperature response using ANOVA; interactions between temperature and substrate concentration were detected only rarely. Kinetic parameters, used to link microbial activity and substrate utilization with temperature sensitivity, exhibited mixed responses to short‐term warming. Maximum specific utilization rates showed the greater temperature sensitivity, with Q 10 values ranging from 0.25 to 13. Specific affinities responded to temperature significantly at only about half of the stations in the polynya. Psychrophilic behaviors (e.g., highest specific affinities and oligotrophic capacities at lower incubation temperatures) were observed at stations most likely to be influenced by direct Arctic Ocean outflow. Complete agreement with the hypothesis of enhanced substrate requirement by bacteria living at subzero temperature was not found. An improved understanding of the diversity of cold‐tolerant and cold‐loving microorganisms is needed before generalizing or predicting the role of temperature in the cycling of organic matter at high latitudes. |
format |
Article in Journal/Newspaper |
author |
Yager, Patricia L. Deming, Jody W. |
author_facet |
Yager, Patricia L. Deming, Jody W. |
author_sort |
Yager, Patricia L. |
title |
Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
title_short |
Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
title_full |
Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
title_fullStr |
Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
title_full_unstemmed |
Pelagic microbial activity in an arctic polynya: Testing for temperature and substrate interactions using a kinetic approach |
title_sort |
pelagic microbial activity in an arctic polynya: testing for temperature and substrate interactions using a kinetic approach |
publisher |
Wiley |
publishDate |
1999 |
url |
http://dx.doi.org/10.4319/lo.1999.44.8.1882 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1999.44.8.1882 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1999.44.8.1882 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Limnology and Oceanography volume 44, issue 8, page 1882-1893 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.1999.44.8.1882 |
container_title |
Limnology and Oceanography |
container_volume |
44 |
container_issue |
8 |
container_start_page |
1882 |
op_container_end_page |
1893 |
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1784264044324061184 |