Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments
The extracellular enzymatic hydrolysis rates of three fluorescently‐labeled polysaccharides (pullulan, laminarin, and xylan) were measured in the upper ∼11 cm of sediment cores collected near Svalbard in the Arctic Ocean. The three polysaccharides differ in molecular weight (200,000, ∼6,000, and ∼8,...
Published in: | Limnology and Oceanography |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1998
|
Subjects: | |
Online Access: | http://dx.doi.org/10.4319/lo.1998.43.2.0315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1998.43.2.0315 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1998.43.2.0315 |
id |
crwiley:10.4319/lo.1998.43.2.0315 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.4319/lo.1998.43.2.0315 2023-12-03T10:17:08+01:00 Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments Arnosti, C. 1998 http://dx.doi.org/10.4319/lo.1998.43.2.0315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1998.43.2.0315 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1998.43.2.0315 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 43, issue 2, page 315-324 ISSN 0024-3590 1939-5590 Aquatic Science Oceanography journal-article 1998 crwiley https://doi.org/10.4319/lo.1998.43.2.0315 2023-11-09T14:19:45Z The extracellular enzymatic hydrolysis rates of three fluorescently‐labeled polysaccharides (pullulan, laminarin, and xylan) were measured in the upper ∼11 cm of sediment cores collected near Svalbard in the Arctic Ocean. The three polysaccharides differ in molecular weight (200,000, ∼6,000, and ∼8,000 Da for pullulan, laminarin, and xylan, respectively), as well as in monomer composition, linkage position, and anomeric configuration, and are most probably hydrolyzed by distinctly different enzymes. Potential hydrolysis rates of pullulan and laminarin were rapid throughout the sediment cores (average rates of 52 cuts per nmol glucose cm −3 hr −1 for pullulan, 38 cuts per nmol glucose cm −3 hr −1 for laminarin) and were comparable to rates measured in sediment cores from more temperate sites. Xylan potential hydrolysis rates were considerably slower at all Svalbard stations (average of 3 cuts per nmol xylose cm −3 hr −1 ). Rapid microbial remineralization of particulate organic carbon requires high levels of extracellular enzyme activity; the high potential hydrolysis rates of tluorescently‐labeled pullulan and laminarin in Svalbard sediments demonstrate that at least some types of extracellular enzymes can function rapidly in permanently cold environments. Article in Journal/Newspaper Arctic Arctic Ocean Svalbard Wiley Online Library (via Crossref) Arctic Arctic Ocean Svalbard Limnology and Oceanography 43 2 315 324 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Oceanography |
spellingShingle |
Aquatic Science Oceanography Arnosti, C. Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
topic_facet |
Aquatic Science Oceanography |
description |
The extracellular enzymatic hydrolysis rates of three fluorescently‐labeled polysaccharides (pullulan, laminarin, and xylan) were measured in the upper ∼11 cm of sediment cores collected near Svalbard in the Arctic Ocean. The three polysaccharides differ in molecular weight (200,000, ∼6,000, and ∼8,000 Da for pullulan, laminarin, and xylan, respectively), as well as in monomer composition, linkage position, and anomeric configuration, and are most probably hydrolyzed by distinctly different enzymes. Potential hydrolysis rates of pullulan and laminarin were rapid throughout the sediment cores (average rates of 52 cuts per nmol glucose cm −3 hr −1 for pullulan, 38 cuts per nmol glucose cm −3 hr −1 for laminarin) and were comparable to rates measured in sediment cores from more temperate sites. Xylan potential hydrolysis rates were considerably slower at all Svalbard stations (average of 3 cuts per nmol xylose cm −3 hr −1 ). Rapid microbial remineralization of particulate organic carbon requires high levels of extracellular enzyme activity; the high potential hydrolysis rates of tluorescently‐labeled pullulan and laminarin in Svalbard sediments demonstrate that at least some types of extracellular enzymes can function rapidly in permanently cold environments. |
format |
Article in Journal/Newspaper |
author |
Arnosti, C. |
author_facet |
Arnosti, C. |
author_sort |
Arnosti, C. |
title |
Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
title_short |
Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
title_full |
Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
title_fullStr |
Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
title_full_unstemmed |
Rapid potential rates of extracellular enzymatic hydrolysis in Arctic sediments |
title_sort |
rapid potential rates of extracellular enzymatic hydrolysis in arctic sediments |
publisher |
Wiley |
publishDate |
1998 |
url |
http://dx.doi.org/10.4319/lo.1998.43.2.0315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1998.43.2.0315 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1998.43.2.0315 |
geographic |
Arctic Arctic Ocean Svalbard |
geographic_facet |
Arctic Arctic Ocean Svalbard |
genre |
Arctic Arctic Ocean Svalbard |
genre_facet |
Arctic Arctic Ocean Svalbard |
op_source |
Limnology and Oceanography volume 43, issue 2, page 315-324 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.1998.43.2.0315 |
container_title |
Limnology and Oceanography |
container_volume |
43 |
container_issue |
2 |
container_start_page |
315 |
op_container_end_page |
324 |
_version_ |
1784264089153830912 |