Discrete dissolved and particulate proteins in oceanic waters
Dissolved and particulate proteins were extracted from samples of surface seawater collected from the equatorial area, through the Indian Ocean, to the Antarctic Ocean. Dissolved proteins were also observed in waters of the equatorial Pacific. Dissolved and particulate proteins with a wide range of...
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crwiley:10.4319/lo.1996.41.6.1334 2024-09-15T17:47:31+00:00 Discrete dissolved and particulate proteins in oceanic waters Tanoue, Eiichiro Ishii, Masao Midorikawa, Takashi 1996 http://dx.doi.org/10.4319/lo.1996.41.6.1334 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1996.41.6.1334 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1996.41.6.1334 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 41, issue 6, page 1334-1343 ISSN 0024-3590 1939-5590 journal-article 1996 crwiley https://doi.org/10.4319/lo.1996.41.6.1334 2024-07-23T04:12:08Z Dissolved and particulate proteins were extracted from samples of surface seawater collected from the equatorial area, through the Indian Ocean, to the Antarctic Ocean. Dissolved proteins were also observed in waters of the equatorial Pacific. Dissolved and particulate proteins with a wide range of molecular masses were detected by sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). The particulate proteins were made up of many background proteins of overlapping molecular weight, which caused uniform staining in the gel. However, distinct bands of individual proteins with apparent molecular masses of ∼66 and 45 kDa were evident among the background proteins. Electrophoretograms of dissolved proteins were quite different from those of the particulate proteins. The dissolved background proteins were not significant, and fewer than 30 proteins were clearly visualized as major dissolved proteins. Dissolved proteins with apparent molecular masses of 48 and 37 kDa were commonly found as major proteins in all samples examined. Such molecular characteristics of dissolved and particulate proteins are consistent with previous results from the North Pacific. Thus, it appears that the processes by which specific proteins from marine organisms are transferred to and accumulated in the pools of dissolved and particulate organic matter are identical throughout the world’s oceans. Article in Journal/Newspaper Antarc* Antarctic Antarctic Ocean Wiley Online Library Limnology and Oceanography 41 6 1334 1343 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Dissolved and particulate proteins were extracted from samples of surface seawater collected from the equatorial area, through the Indian Ocean, to the Antarctic Ocean. Dissolved proteins were also observed in waters of the equatorial Pacific. Dissolved and particulate proteins with a wide range of molecular masses were detected by sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). The particulate proteins were made up of many background proteins of overlapping molecular weight, which caused uniform staining in the gel. However, distinct bands of individual proteins with apparent molecular masses of ∼66 and 45 kDa were evident among the background proteins. Electrophoretograms of dissolved proteins were quite different from those of the particulate proteins. The dissolved background proteins were not significant, and fewer than 30 proteins were clearly visualized as major dissolved proteins. Dissolved proteins with apparent molecular masses of 48 and 37 kDa were commonly found as major proteins in all samples examined. Such molecular characteristics of dissolved and particulate proteins are consistent with previous results from the North Pacific. Thus, it appears that the processes by which specific proteins from marine organisms are transferred to and accumulated in the pools of dissolved and particulate organic matter are identical throughout the world’s oceans. |
format |
Article in Journal/Newspaper |
author |
Tanoue, Eiichiro Ishii, Masao Midorikawa, Takashi |
spellingShingle |
Tanoue, Eiichiro Ishii, Masao Midorikawa, Takashi Discrete dissolved and particulate proteins in oceanic waters |
author_facet |
Tanoue, Eiichiro Ishii, Masao Midorikawa, Takashi |
author_sort |
Tanoue, Eiichiro |
title |
Discrete dissolved and particulate proteins in oceanic waters |
title_short |
Discrete dissolved and particulate proteins in oceanic waters |
title_full |
Discrete dissolved and particulate proteins in oceanic waters |
title_fullStr |
Discrete dissolved and particulate proteins in oceanic waters |
title_full_unstemmed |
Discrete dissolved and particulate proteins in oceanic waters |
title_sort |
discrete dissolved and particulate proteins in oceanic waters |
publisher |
Wiley |
publishDate |
1996 |
url |
http://dx.doi.org/10.4319/lo.1996.41.6.1334 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1996.41.6.1334 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1996.41.6.1334 |
genre |
Antarc* Antarctic Antarctic Ocean |
genre_facet |
Antarc* Antarctic Antarctic Ocean |
op_source |
Limnology and Oceanography volume 41, issue 6, page 1334-1343 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.1996.41.6.1334 |
container_title |
Limnology and Oceanography |
container_volume |
41 |
container_issue |
6 |
container_start_page |
1334 |
op_container_end_page |
1343 |
_version_ |
1810496909849133056 |