Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific
The purpose of this study was to test the hypothesis that NH 4 + uptake by heterotrophic bacteria in the subarctic Pacific is C limited. Addition of glucose (0.5–1.0 µ M) stimulted NH 4 + uptake in unfractionated water but had no effect on bacterial abundance. Glucose stimulation of NH 4 + uptake wa...
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Language: | English |
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crwiley:10.4319/lo.1990.35.6.1258 2024-06-23T07:57:00+00:00 Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific Kirchman, David L. Keil, Richard G. Wheeler, Patricia A. 1990 http://dx.doi.org/10.4319/lo.1990.35.6.1258 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1990.35.6.1258 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1990.35.6.1258 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 35, issue 6, page 1258-1266 ISSN 0024-3590 1939-5590 journal-article 1990 crwiley https://doi.org/10.4319/lo.1990.35.6.1258 2024-06-11T04:40:27Z The purpose of this study was to test the hypothesis that NH 4 + uptake by heterotrophic bacteria in the subarctic Pacific is C limited. Addition of glucose (0.5–1.0 µ M) stimulted NH 4 + uptake in unfractionated water but had no effect on bacterial abundance. Glucose stimulation of NH 4 + uptake was even greater in the bacterial size fraction (<0.8 µ m). Regeneration of 15 NH 4 + from added [ 15 N] amino acids was measurable in the bacterial size fraction, but glucose additions prevented net NH 4 + excretion because of increased uptake of NH 4 + . In the subarctic Pacific, NH 4 + concentrations range from undetectable (≤0.05 µ M) to 0.4 µ M, whereas the maximal estimate of monosaccharide concentrations was 0.025 M. These results indicate that the supply of C compounds like glucose can limit NH 4 + uptake by heterotrophic bacteria. Article in Journal/Newspaper Subarctic Wiley Online Library Pacific Limnology and Oceanography 35 6 1258 1266 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
The purpose of this study was to test the hypothesis that NH 4 + uptake by heterotrophic bacteria in the subarctic Pacific is C limited. Addition of glucose (0.5–1.0 µ M) stimulted NH 4 + uptake in unfractionated water but had no effect on bacterial abundance. Glucose stimulation of NH 4 + uptake was even greater in the bacterial size fraction (<0.8 µ m). Regeneration of 15 NH 4 + from added [ 15 N] amino acids was measurable in the bacterial size fraction, but glucose additions prevented net NH 4 + excretion because of increased uptake of NH 4 + . In the subarctic Pacific, NH 4 + concentrations range from undetectable (≤0.05 µ M) to 0.4 µ M, whereas the maximal estimate of monosaccharide concentrations was 0.025 M. These results indicate that the supply of C compounds like glucose can limit NH 4 + uptake by heterotrophic bacteria. |
format |
Article in Journal/Newspaper |
author |
Kirchman, David L. Keil, Richard G. Wheeler, Patricia A. |
spellingShingle |
Kirchman, David L. Keil, Richard G. Wheeler, Patricia A. Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
author_facet |
Kirchman, David L. Keil, Richard G. Wheeler, Patricia A. |
author_sort |
Kirchman, David L. |
title |
Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
title_short |
Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
title_full |
Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
title_fullStr |
Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
title_full_unstemmed |
Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific |
title_sort |
carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic pacific |
publisher |
Wiley |
publishDate |
1990 |
url |
http://dx.doi.org/10.4319/lo.1990.35.6.1258 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1990.35.6.1258 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1990.35.6.1258 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Limnology and Oceanography volume 35, issue 6, page 1258-1266 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.1990.35.6.1258 |
container_title |
Limnology and Oceanography |
container_volume |
35 |
container_issue |
6 |
container_start_page |
1258 |
op_container_end_page |
1266 |
_version_ |
1802650442978557952 |