STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1
To test the applicability of substrate uptake kinetics for assessing rates of microbial heterotrophic activity in the sea, the conformity between accepted notations for pure enzyme systems as opposed to natural populations is examined. Minimum rates for the in situ uptake of organic carbon are calcu...
Published in: | Limnology and Oceanography |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1966
|
Subjects: | |
Online Access: | http://dx.doi.org/10.4319/lo.1966.11.4.0596 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1966.11.4.0596 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1966.11.4.0596 |
id |
crwiley:10.4319/lo.1966.11.4.0596 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.4319/lo.1966.11.4.0596 2024-03-31T07:55:22+00:00 STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 Vaccaro, Ralph F. Jannasch, Holger W. 1966 http://dx.doi.org/10.4319/lo.1966.11.4.0596 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1966.11.4.0596 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1966.11.4.0596 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 11, issue 4, page 596-607 ISSN 0024-3590 1939-5590 Aquatic Science Oceanography journal-article 1966 crwiley https://doi.org/10.4319/lo.1966.11.4.0596 2024-03-04T13:02:55Z To test the applicability of substrate uptake kinetics for assessing rates of microbial heterotrophic activity in the sea, the conformity between accepted notations for pure enzyme systems as opposed to natural populations is examined. Minimum rates for the in situ uptake of organic carbon are calculated using 14 C‐labeled glucose as a substrate. To provide an estimate of naturally occurring substrate, a complementary bioassay procedure is applied to complete the analysis. The isolation and the uptake characteristics of the bioassay organism, which resembles Achromobacter aquamarinus, and its capability of discerning glucose at concentrations as low as 4 × 10 −8 m are discussed. Although the reliability of kinetic constants cannot be assured in multisample situations because of the possibility of inherent sample differences, we have applied the complete analysis to coastal and offshore waters to examine the magnitude and internal consistency of the results. The results showed clearly the influence of nutrient‐poor Amazon River water in offshore regions of the South Atlantic Ocean and suggest the possibility of combining uptake measurements of natural populations with those of pure cultures of a test organism. The necessity for an improved direct analysis for more reliable estimates of the natural substrate concentration remains. Article in Journal/Newspaper South Atlantic Ocean Wiley Online Library Limnology and Oceanography 11 4 596 607 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Oceanography |
spellingShingle |
Aquatic Science Oceanography Vaccaro, Ralph F. Jannasch, Holger W. STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
topic_facet |
Aquatic Science Oceanography |
description |
To test the applicability of substrate uptake kinetics for assessing rates of microbial heterotrophic activity in the sea, the conformity between accepted notations for pure enzyme systems as opposed to natural populations is examined. Minimum rates for the in situ uptake of organic carbon are calculated using 14 C‐labeled glucose as a substrate. To provide an estimate of naturally occurring substrate, a complementary bioassay procedure is applied to complete the analysis. The isolation and the uptake characteristics of the bioassay organism, which resembles Achromobacter aquamarinus, and its capability of discerning glucose at concentrations as low as 4 × 10 −8 m are discussed. Although the reliability of kinetic constants cannot be assured in multisample situations because of the possibility of inherent sample differences, we have applied the complete analysis to coastal and offshore waters to examine the magnitude and internal consistency of the results. The results showed clearly the influence of nutrient‐poor Amazon River water in offshore regions of the South Atlantic Ocean and suggest the possibility of combining uptake measurements of natural populations with those of pure cultures of a test organism. The necessity for an improved direct analysis for more reliable estimates of the natural substrate concentration remains. |
format |
Article in Journal/Newspaper |
author |
Vaccaro, Ralph F. Jannasch, Holger W. |
author_facet |
Vaccaro, Ralph F. Jannasch, Holger W. |
author_sort |
Vaccaro, Ralph F. |
title |
STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
title_short |
STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
title_full |
STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
title_fullStr |
STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
title_full_unstemmed |
STUDIES ON HETEROTROPHIC ACTIVITY IN SEAWATER BASED ON GLUCOSE ASSIMILATION1 |
title_sort |
studies on heterotrophic activity in seawater based on glucose assimilation1 |
publisher |
Wiley |
publishDate |
1966 |
url |
http://dx.doi.org/10.4319/lo.1966.11.4.0596 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.1966.11.4.0596 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.1966.11.4.0596 |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_source |
Limnology and Oceanography volume 11, issue 4, page 596-607 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.1966.11.4.0596 |
container_title |
Limnology and Oceanography |
container_volume |
11 |
container_issue |
4 |
container_start_page |
596 |
op_container_end_page |
607 |
_version_ |
1795037164118999040 |