Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae)
• Premise of the study: Microsatellite primers were developed for the endophytic fungus Epichloë festucae , which is symbiotic with Festuca rubra , to study the population genetics of the species and to compare population structures between E. festucae and its host F. rubra . • Methods and Results:...
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crwiley:10.3732/apps.1400093 2023-12-03T10:22:28+01:00 Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Öhberg, Annika Saikkonen, Kari 2014 http://dx.doi.org/10.3732/apps.1400093 http://www.bioone.org/doi/full-xml/10.3732/apps.1400093 https://onlinelibrary.wiley.com/doi/full/10.3732/apps.1400093 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Applications in Plant Sciences volume 2, issue 12 ISSN 2168-0450 2168-0450 Plant Science Ecology, Evolution, Behavior and Systematics journal-article 2014 crwiley https://doi.org/10.3732/apps.1400093 2023-11-09T14:04:21Z • Premise of the study: Microsatellite primers were developed for the endophytic fungus Epichloë festucae , which is symbiotic with Festuca rubra , to study the population genetics of the species and to compare population structures between E. festucae and its host F. rubra . • Methods and Results: We developed 14 polymorphic markers using the unplaced genomic scaffold sequences of E. festucae from GenBank. The number of alleles per locus ( A ) varied from four to 16, and unbiased haploid diversity ( h ) was 0.717 in eight populations located in the Faroe Islands, Finland, and Spain. The Spanish populations possessed a higher number of alleles and haploid diversity (on average A = 5.1 and h = 0.591, respectively) compared to northern populations (on average A = 1.5 and h = 0.199, respectively). • Conclusions: These polymorphic markers will be used by grass breeders for uses including the improvement of commercial turfgrass cultivars, and by population geneticists to study different species of the Epichloë genus. Article in Journal/Newspaper Faroe Islands Wiley Online Library (via Crossref) Faroe Islands Applications in Plant Sciences 2 12 |
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Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Plant Science Ecology, Evolution, Behavior and Systematics |
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Plant Science Ecology, Evolution, Behavior and Systematics von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Öhberg, Annika Saikkonen, Kari Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
topic_facet |
Plant Science Ecology, Evolution, Behavior and Systematics |
description |
• Premise of the study: Microsatellite primers were developed for the endophytic fungus Epichloë festucae , which is symbiotic with Festuca rubra , to study the population genetics of the species and to compare population structures between E. festucae and its host F. rubra . • Methods and Results: We developed 14 polymorphic markers using the unplaced genomic scaffold sequences of E. festucae from GenBank. The number of alleles per locus ( A ) varied from four to 16, and unbiased haploid diversity ( h ) was 0.717 in eight populations located in the Faroe Islands, Finland, and Spain. The Spanish populations possessed a higher number of alleles and haploid diversity (on average A = 5.1 and h = 0.591, respectively) compared to northern populations (on average A = 1.5 and h = 0.199, respectively). • Conclusions: These polymorphic markers will be used by grass breeders for uses including the improvement of commercial turfgrass cultivars, and by population geneticists to study different species of the Epichloë genus. |
format |
Article in Journal/Newspaper |
author |
von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Öhberg, Annika Saikkonen, Kari |
author_facet |
von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Öhberg, Annika Saikkonen, Kari |
author_sort |
von Cräutlein, Maria |
title |
Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
title_short |
Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
title_full |
Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
title_fullStr |
Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
title_full_unstemmed |
Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloë festucae (Clavicipitaceae) |
title_sort |
development and characterization of nuclear microsatellite markers in the endophytic fungus epichloë festucae (clavicipitaceae) |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.3732/apps.1400093 http://www.bioone.org/doi/full-xml/10.3732/apps.1400093 https://onlinelibrary.wiley.com/doi/full/10.3732/apps.1400093 |
geographic |
Faroe Islands |
geographic_facet |
Faroe Islands |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
Applications in Plant Sciences volume 2, issue 12 ISSN 2168-0450 2168-0450 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.3732/apps.1400093 |
container_title |
Applications in Plant Sciences |
container_volume |
2 |
container_issue |
12 |
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1784270387627950080 |