High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae)
• Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic‐alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species....
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Online Access: | http://dx.doi.org/10.3732/apps.1300002 http://www.bioone.org/doi/full-xml/10.3732/apps.1300002 http://www.bioone.org/doi/pdf/10.3732/apps.1300002 |
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crwiley:10.3732/apps.1300002 2024-06-23T07:49:48+00:00 High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) Matheny, Hannah Edwards, Joan Maroja, Luana S. National Park Service 2013 http://dx.doi.org/10.3732/apps.1300002 http://www.bioone.org/doi/full-xml/10.3732/apps.1300002 http://www.bioone.org/doi/pdf/10.3732/apps.1300002 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Applications in Plant Sciences volume 1, issue 8 ISSN 2168-0450 2168-0450 journal-article 2013 crwiley https://doi.org/10.3732/apps.1300002 2024-06-06T04:23:07Z • Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic‐alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. • Methods and Results: We used Roche/454 high‐throughput technology to sequence microsatellite‐enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di‐, tri‐, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. • Conclusions: Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change. Article in Journal/Newspaper Arctic Climate change Wiley Online Library Arctic Applications in Plant Sciences 1 8 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
• Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic‐alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. • Methods and Results: We used Roche/454 high‐throughput technology to sequence microsatellite‐enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di‐, tri‐, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. • Conclusions: Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change. |
author2 |
National Park Service |
format |
Article in Journal/Newspaper |
author |
Matheny, Hannah Edwards, Joan Maroja, Luana S. |
spellingShingle |
Matheny, Hannah Edwards, Joan Maroja, Luana S. High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
author_facet |
Matheny, Hannah Edwards, Joan Maroja, Luana S. |
author_sort |
Matheny, Hannah |
title |
High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
title_short |
High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
title_full |
High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
title_fullStr |
High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
title_full_unstemmed |
High‐throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae) |
title_sort |
high‐throughput microsatellite marker development for the distylous herb primula mistassinica (primulaceae) |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.3732/apps.1300002 http://www.bioone.org/doi/full-xml/10.3732/apps.1300002 http://www.bioone.org/doi/pdf/10.3732/apps.1300002 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_source |
Applications in Plant Sciences volume 1, issue 8 ISSN 2168-0450 2168-0450 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.3732/apps.1300002 |
container_title |
Applications in Plant Sciences |
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1 |
container_issue |
8 |
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1802640476364341248 |