Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation
Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic‐Alpine plant species. Sequences of the psb A– trn H intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1–5.8S–ITS2, of the nuclear ribosomal DNA were used to investiga...
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crwiley:10.3732/ajb.90.6.931 2024-09-15T18:33:56+00:00 Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation Holderegger, Rolf Abbott, Richard J. Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 2003 http://dx.doi.org/10.3732/ajb.90.6.931 https://onlinelibrary.wiley.com/doi/full/10.3732/ajb.90.6.931 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor American Journal of Botany volume 90, issue 6, page 931-936 ISSN 0002-9122 1537-2197 journal-article 2003 crwiley https://doi.org/10.3732/ajb.90.6.931 2024-07-09T04:12:53Z Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic‐Alpine plant species. Sequences of the psb A– trn H intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1–5.8S–ITS2, of the nuclear ribosomal DNA were used to investigate the intraspecific evolution and phylogeography of this species. Samples from nearly the species' entire circumpolar distribution were included in the analysis as well as samples from the closely related taxa S. smalliana from Alaska and S. blepharophylla and S. biflora from the Alps ( S. aizoides served as outgroup). These latter taxa showed a low number of parsimony informative characters, in both cpDNA and ITS sequences, which separated them from S. oppositifolia . Two main cpDNA haplotypes were detected within S. oppositifolia , one with a Eurasian distribution and one with an East Asian‐North American distribution. This confirmed the existence of two cpDNA lineages with different geographical distributions in this species, which had previously been reported based on a RFLP analysis. The ITS phylogeny was not useful with respect to the intraspecific evolution and phylogeography of S. oppositifolia , because it showed a largely unresolved topology with low statistical support. The cpDNA sequence analysis, however, also suggested a putative long‐distance dispersal event. All investigated taxa had cpDNA haplotypes that were congruent with their geographical origin rather than their phylogeny. This could point to putative lineage sorting in S. oppositifolia and related taxa. Article in Journal/Newspaper Saxifraga oppositifolia Alaska Wiley Online Library American Journal of Botany 90 6 931 936 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic‐Alpine plant species. Sequences of the psb A– trn H intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1–5.8S–ITS2, of the nuclear ribosomal DNA were used to investigate the intraspecific evolution and phylogeography of this species. Samples from nearly the species' entire circumpolar distribution were included in the analysis as well as samples from the closely related taxa S. smalliana from Alaska and S. blepharophylla and S. biflora from the Alps ( S. aizoides served as outgroup). These latter taxa showed a low number of parsimony informative characters, in both cpDNA and ITS sequences, which separated them from S. oppositifolia . Two main cpDNA haplotypes were detected within S. oppositifolia , one with a Eurasian distribution and one with an East Asian‐North American distribution. This confirmed the existence of two cpDNA lineages with different geographical distributions in this species, which had previously been reported based on a RFLP analysis. The ITS phylogeny was not useful with respect to the intraspecific evolution and phylogeography of S. oppositifolia , because it showed a largely unresolved topology with low statistical support. The cpDNA sequence analysis, however, also suggested a putative long‐distance dispersal event. All investigated taxa had cpDNA haplotypes that were congruent with their geographical origin rather than their phylogeny. This could point to putative lineage sorting in S. oppositifolia and related taxa. |
author2 |
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung |
format |
Article in Journal/Newspaper |
author |
Holderegger, Rolf Abbott, Richard J. |
spellingShingle |
Holderegger, Rolf Abbott, Richard J. Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
author_facet |
Holderegger, Rolf Abbott, Richard J. |
author_sort |
Holderegger, Rolf |
title |
Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
title_short |
Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
title_full |
Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
title_fullStr |
Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
title_full_unstemmed |
Phylogeography of the Arctic‐Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and ITS sequence variation |
title_sort |
phylogeography of the arctic‐alpine saxifraga oppositifolia (saxifragaceae) and some related taxa based on cpdna and its sequence variation |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.3732/ajb.90.6.931 https://onlinelibrary.wiley.com/doi/full/10.3732/ajb.90.6.931 |
genre |
Saxifraga oppositifolia Alaska |
genre_facet |
Saxifraga oppositifolia Alaska |
op_source |
American Journal of Botany volume 90, issue 6, page 931-936 ISSN 0002-9122 1537-2197 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.3732/ajb.90.6.931 |
container_title |
American Journal of Botany |
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90 |
container_issue |
6 |
container_start_page |
931 |
op_container_end_page |
936 |
_version_ |
1810475669181693952 |