Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska
Taxonomic consensus is lacking on the Oxytropis arctica and O. campestris species complexes, two polyploid complexes found in the interior and arctic areas of Alaska. One classification has emphasized flower size, whereas flower color is considered a key diagnostic character in another classificatio...
Published in: | American Journal of Botany |
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crwiley:10.3732/ajb.90.10.1470 2024-09-09T19:23:19+00:00 Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska Jorgensen, Janet L. Stehlik, Ivana Brochmann, Christian Conti, Elena U.S. Air Force 2003 http://dx.doi.org/10.3732/ajb.90.10.1470 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.3732%2Fajb.90.10.1470 https://onlinelibrary.wiley.com/doi/pdf/10.3732/ajb.90.10.1470 https://onlinelibrary.wiley.com/doi/full-xml/10.3732/ajb.90.10.1470 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor American Journal of Botany volume 90, issue 10, page 1470-1480 ISSN 0002-9122 1537-2197 journal-article 2003 crwiley https://doi.org/10.3732/ajb.90.10.1470 2024-07-18T04:26:00Z Taxonomic consensus is lacking on the Oxytropis arctica and O. campestris species complexes, two polyploid complexes found in the interior and arctic areas of Alaska. One classification has emphasized flower size, whereas flower color is considered a key diagnostic character in another classification. Our analyses of internal transcribed spacer (ITS) sequences and random amplified polymorphic DNA (RAPD) markers provided no support for either classification system. The trees generated from ITS sequences and the phenogram derived from RAPD markers suggest that most recognized taxa in the two complexes are probably polyphyletic, including O. arctica var. barnebyana , which is listed as threatened in Alaska. The only consistent pattern detected by both types of molecular markers was a geographic split dividing the northeastern arctic populations from most other populations (48.60–55.03% in AMOVA analyses). This genetic subdivision probably reflects a Pleistocene barrier formed by the northern coastal ice shield. Our molecular data, in conjunction with the previously reported variation of ploidy levels in these groups, suggest a scenario of recent and multiple origins of polyploidy. It is possible that most Alaskan populations of these two complexes are best referred to as a single taxonomic species despite morphological differentiation within the complexes. Article in Journal/Newspaper Arctic Alaska Wiley Online Library Arctic American Journal of Botany 90 10 1470 1480 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Taxonomic consensus is lacking on the Oxytropis arctica and O. campestris species complexes, two polyploid complexes found in the interior and arctic areas of Alaska. One classification has emphasized flower size, whereas flower color is considered a key diagnostic character in another classification. Our analyses of internal transcribed spacer (ITS) sequences and random amplified polymorphic DNA (RAPD) markers provided no support for either classification system. The trees generated from ITS sequences and the phenogram derived from RAPD markers suggest that most recognized taxa in the two complexes are probably polyphyletic, including O. arctica var. barnebyana , which is listed as threatened in Alaska. The only consistent pattern detected by both types of molecular markers was a geographic split dividing the northeastern arctic populations from most other populations (48.60–55.03% in AMOVA analyses). This genetic subdivision probably reflects a Pleistocene barrier formed by the northern coastal ice shield. Our molecular data, in conjunction with the previously reported variation of ploidy levels in these groups, suggest a scenario of recent and multiple origins of polyploidy. It is possible that most Alaskan populations of these two complexes are best referred to as a single taxonomic species despite morphological differentiation within the complexes. |
author2 |
U.S. Air Force |
format |
Article in Journal/Newspaper |
author |
Jorgensen, Janet L. Stehlik, Ivana Brochmann, Christian Conti, Elena |
spellingShingle |
Jorgensen, Janet L. Stehlik, Ivana Brochmann, Christian Conti, Elena Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
author_facet |
Jorgensen, Janet L. Stehlik, Ivana Brochmann, Christian Conti, Elena |
author_sort |
Jorgensen, Janet L. |
title |
Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
title_short |
Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
title_full |
Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
title_fullStr |
Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
title_full_unstemmed |
Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestrisand O. arctica(Fabaceae) complexes in Alaska |
title_sort |
implications of its sequences and rapd markers for the taxonomy and biogeography of the oxytropis campestrisand o. arctica(fabaceae) complexes in alaska |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.3732/ajb.90.10.1470 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.3732%2Fajb.90.10.1470 https://onlinelibrary.wiley.com/doi/pdf/10.3732/ajb.90.10.1470 https://onlinelibrary.wiley.com/doi/full-xml/10.3732/ajb.90.10.1470 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_source |
American Journal of Botany volume 90, issue 10, page 1470-1480 ISSN 0002-9122 1537-2197 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.3732/ajb.90.10.1470 |
container_title |
American Journal of Botany |
container_volume |
90 |
container_issue |
10 |
container_start_page |
1470 |
op_container_end_page |
1480 |
_version_ |
1809763726574747648 |