Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae)
We developed a combined molecular and morphological approach to unravel complex variation at low taxonomic levels, exemplified by some arctic members of Potentilla. Twenty‐one populations from Svalbard were analyzed for random amplified polymorphic DNAs (RAPDs) and 64 morphological characters to tes...
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crwiley:10.2307/2656873 2024-10-13T14:05:15+00:00 Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) Hansen, Kjell Tore Elven, Reidar Brochmann, Christian Universitetet i Oslo Norges Forskningsråd 2000 http://dx.doi.org/10.2307/2656873 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2307%2F2656873 http://onlinelibrary.wiley.com/wol1/doi/10.2307/2656873/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor American Journal of Botany volume 87, issue 10, page 1466-1479 ISSN 0002-9122 1537-2197 journal-article 2000 crwiley https://doi.org/10.2307/2656873 2024-09-19T04:19:14Z We developed a combined molecular and morphological approach to unravel complex variation at low taxonomic levels, exemplified by some arctic members of Potentilla. Twenty‐one populations from Svalbard were analyzed for random amplified polymorphic DNAs (RAPDs) and 64 morphological characters to test the hypotheses that (1) the P. nivea complex (section Niveae ) consists of three taxa ( P. chamissonis, P. insularis , and P. nivea ), (2) three “eco‐morphotypes” in P. pulchella (section Multifidae ) should be considered different taxa, and (3) P. insularis originated as an intersectional hybrid ( Niveae × Multifidae ). Twenty‐two RAPD multilocus phenotypes were observed in the 136 plants analyzed based on 35 markers. Three fairly distinct groups of RAPD phenotypes were identified in the P. nivea complex based on multivariate analyses and an analysis of molecular variance (AMOVA; 77.6% among‐ group variation). The variation within the P. nivea complex was more or less continuous in multivariate analyses of the morphological data. We identified, however, several individual morphological characters that separated unambiguously among the three groups of RAPD phenotypes, revealing that these groups correspond to the previously hypothesized taxa. Many identical RAPD multilocus phenotypes were observed in the “eco‐morphotypes” of P. pulchella , suggesting that its conspicuous morphological variation is caused by plasticity or by genetic variation at a small number of loci. The hypothesis of the hybrid origin of P. insularis was not supported by the RAPD data. Overall, very little RAPD variation was observed within populations of the four taxa (2.1–16.7% in AMOVA analyses; average genotypic diversity, D , was 0.10–0.30). We conclude that detailed, concerted analysis of molecules and morphology is a powerful tool in low‐level taxonomy. Article in Journal/Newspaper Arctic Svalbard Wiley Online Library Arctic Nivea ENVELOPE(-45.479,-45.479,-60.580,-60.580) Svalbard American Journal of Botany 87 10 1466 1479 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
We developed a combined molecular and morphological approach to unravel complex variation at low taxonomic levels, exemplified by some arctic members of Potentilla. Twenty‐one populations from Svalbard were analyzed for random amplified polymorphic DNAs (RAPDs) and 64 morphological characters to test the hypotheses that (1) the P. nivea complex (section Niveae ) consists of three taxa ( P. chamissonis, P. insularis , and P. nivea ), (2) three “eco‐morphotypes” in P. pulchella (section Multifidae ) should be considered different taxa, and (3) P. insularis originated as an intersectional hybrid ( Niveae × Multifidae ). Twenty‐two RAPD multilocus phenotypes were observed in the 136 plants analyzed based on 35 markers. Three fairly distinct groups of RAPD phenotypes were identified in the P. nivea complex based on multivariate analyses and an analysis of molecular variance (AMOVA; 77.6% among‐ group variation). The variation within the P. nivea complex was more or less continuous in multivariate analyses of the morphological data. We identified, however, several individual morphological characters that separated unambiguously among the three groups of RAPD phenotypes, revealing that these groups correspond to the previously hypothesized taxa. Many identical RAPD multilocus phenotypes were observed in the “eco‐morphotypes” of P. pulchella , suggesting that its conspicuous morphological variation is caused by plasticity or by genetic variation at a small number of loci. The hypothesis of the hybrid origin of P. insularis was not supported by the RAPD data. Overall, very little RAPD variation was observed within populations of the four taxa (2.1–16.7% in AMOVA analyses; average genotypic diversity, D , was 0.10–0.30). We conclude that detailed, concerted analysis of molecules and morphology is a powerful tool in low‐level taxonomy. |
author2 |
Universitetet i Oslo Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Hansen, Kjell Tore Elven, Reidar Brochmann, Christian |
spellingShingle |
Hansen, Kjell Tore Elven, Reidar Brochmann, Christian Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
author_facet |
Hansen, Kjell Tore Elven, Reidar Brochmann, Christian |
author_sort |
Hansen, Kjell Tore |
title |
Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
title_short |
Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
title_full |
Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
title_fullStr |
Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
title_full_unstemmed |
Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae) |
title_sort |
molecules and morphology in concert: tests of some hypotheses in arctic potentilla (rosaceae) |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.2307/2656873 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2307%2F2656873 http://onlinelibrary.wiley.com/wol1/doi/10.2307/2656873/fullpdf |
long_lat |
ENVELOPE(-45.479,-45.479,-60.580,-60.580) |
geographic |
Arctic Nivea Svalbard |
geographic_facet |
Arctic Nivea Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
American Journal of Botany volume 87, issue 10, page 1466-1479 ISSN 0002-9122 1537-2197 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.2307/2656873 |
container_title |
American Journal of Botany |
container_volume |
87 |
container_issue |
10 |
container_start_page |
1466 |
op_container_end_page |
1479 |
_version_ |
1812811317166211072 |