Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring
ABSTRACT Noninvasive genetic monitoring of animal populations has become a widely used method in animal conservation and wildlife management due to its known advantages in sample availability of endangered or elusive species. A variety of methods have been suggested to overcome the difficulties of c...
Published in: | The Journal of Wildlife Management |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2010
|
Subjects: | |
Online Access: | http://dx.doi.org/10.2193/2009-421 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2193%2F2009-421 |
id |
crwiley:10.2193/2009-421 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.2193/2009-421 2024-04-28T08:15:27+00:00 Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring Hausknecht, Roland Bayerl, Helmut Gula, Roman Kuehn, Ralph 2010 http://dx.doi.org/10.2193/2009-421 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2193%2F2009-421 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The Journal of Wildlife Management volume 74, issue 8, page 1904-1910 ISSN 0022-541X 1937-2817 Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics journal-article 2010 crwiley https://doi.org/10.2193/2009-421 2024-04-05T07:40:30Z ABSTRACT Noninvasive genetic monitoring of animal populations has become a widely used method in animal conservation and wildlife management due to its known advantages in sample availability of endangered or elusive species. A variety of methods have been suggested to overcome the difficulties of collecting reliable genetic data despite poor DNA quality and quantity of samples. We used quantitative real‐time polymerase chain reaction (qPCR) to quantify DNA contents and preselect extracts suitable for microsatellite genotyping of noninvasive samples from 2 carnivore species, wolf ( Canis lupus ) and Eurasian otter ( Lutra lutra ). We tested 2 concentration thresholds for DNA extracts containing either 5 pg/μL or 25 pg/μL at minimum and evaluated the effect of excluding samples from genotyping falling below either of these DNA concentrations. Depending on species and threshold concentration applied, we reduced the genotyping effort by 21% to 47% and genotyping errors by 7% to 45%, yet we could still detect 82% to 99% of available genotypes. Thus, qPCR may potentially reduce genotyping effort and enhance data reliability in noninvasive genetic studies. Genetic laboratories working on noninvasive population genetic studies could transfer this approach to other species, streamline genetic analyses and, thus, more efficiently provide wildlife managers with reliable genetic data of wild populations. Article in Journal/Newspaper Canis lupus Lutra lutra Wiley Online Library The Journal of Wildlife Management 74 8 1904 1910 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Hausknecht, Roland Bayerl, Helmut Gula, Roman Kuehn, Ralph Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
topic_facet |
Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics |
description |
ABSTRACT Noninvasive genetic monitoring of animal populations has become a widely used method in animal conservation and wildlife management due to its known advantages in sample availability of endangered or elusive species. A variety of methods have been suggested to overcome the difficulties of collecting reliable genetic data despite poor DNA quality and quantity of samples. We used quantitative real‐time polymerase chain reaction (qPCR) to quantify DNA contents and preselect extracts suitable for microsatellite genotyping of noninvasive samples from 2 carnivore species, wolf ( Canis lupus ) and Eurasian otter ( Lutra lutra ). We tested 2 concentration thresholds for DNA extracts containing either 5 pg/μL or 25 pg/μL at minimum and evaluated the effect of excluding samples from genotyping falling below either of these DNA concentrations. Depending on species and threshold concentration applied, we reduced the genotyping effort by 21% to 47% and genotyping errors by 7% to 45%, yet we could still detect 82% to 99% of available genotypes. Thus, qPCR may potentially reduce genotyping effort and enhance data reliability in noninvasive genetic studies. Genetic laboratories working on noninvasive population genetic studies could transfer this approach to other species, streamline genetic analyses and, thus, more efficiently provide wildlife managers with reliable genetic data of wild populations. |
format |
Article in Journal/Newspaper |
author |
Hausknecht, Roland Bayerl, Helmut Gula, Roman Kuehn, Ralph |
author_facet |
Hausknecht, Roland Bayerl, Helmut Gula, Roman Kuehn, Ralph |
author_sort |
Hausknecht, Roland |
title |
Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
title_short |
Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
title_full |
Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
title_fullStr |
Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
title_full_unstemmed |
Application of Quantitative Real‐Time Polymerase Chain Reaction for Noninvasive Genetic Monitoring |
title_sort |
application of quantitative real‐time polymerase chain reaction for noninvasive genetic monitoring |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.2193/2009-421 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2193%2F2009-421 |
genre |
Canis lupus Lutra lutra |
genre_facet |
Canis lupus Lutra lutra |
op_source |
The Journal of Wildlife Management volume 74, issue 8, page 1904-1910 ISSN 0022-541X 1937-2817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.2193/2009-421 |
container_title |
The Journal of Wildlife Management |
container_volume |
74 |
container_issue |
8 |
container_start_page |
1904 |
op_container_end_page |
1910 |
_version_ |
1797581006227636224 |