Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams
Abstract The development of sensitive, biologically based indicators of contaminant exposure (i.e., biomarkers) is an ongoing topic of research. These indicators have been proposed as a first‐tier method of identifying contaminant exposure. The primary objective of this research was to implement a b...
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crwiley:10.1897/05-137r.1 2024-06-23T07:51:23+00:00 Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams Roberts, Aaron P. Oris, James T. Burton, G. Allen Clements, William H. 2005 http://dx.doi.org/10.1897/05-137r.1 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1897%2F05-137R.1 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1897/05-137R.1 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 24, issue 12, page 3092-3098 ISSN 0730-7268 1552-8618 journal-article 2005 crwiley https://doi.org/10.1897/05-137r.1 2024-06-11T04:45:46Z Abstract The development of sensitive, biologically based indicators of contaminant exposure (i.e., biomarkers) is an ongoing topic of research. These indicators have been proposed as a first‐tier method of identifying contaminant exposure. The primary objective of this research was to implement a biomarker‐based method of exposure assessment using caged fish and real‐time reverse‐transcriptase polymerase chain reaction (rtRT‐PCR) measurements of gene expression. Primers were developed for the CYPIA, metallothionein, and vitellogenin genes in rainbow trout ( Oncorhynchyus mykiss ), cutbow trout ( Oncorhynchyus clarkii × mykiss ), and Atlantic salmon ( Salmo salar ). Each of these genes has been shown to respond specifically to planar aromatic compounds, heavy metals, and environmental estrogens, respectively. Juvenile fish were placed in cages and exposed in situ at reference and contaminated sites on the Cache la Poudre River (CO, USA), the Arkansas River (CO, USA), the St. John River (NB, Canada), and two urban creeks near Dayton (OH, USA). Quantitative gene expression was determined using rtRT‐PCR. Biomarker expression profiles were obtained that demonstrated differences in CYP1A, metallothionein, and vitellogenin mRNA production unique to each site, indicating that specific types of compounds were bioavailable and present in sufficient concentrations to elicit transcriptional responses in the organism. These findings support the use of a biomarker‐based approach to exposure identification and assessment. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Canada Dayton ENVELOPE(-158.683,-158.683,-85.733,-85.733) Environmental Toxicology and Chemistry 24 12 3092 3098 |
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English |
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Abstract The development of sensitive, biologically based indicators of contaminant exposure (i.e., biomarkers) is an ongoing topic of research. These indicators have been proposed as a first‐tier method of identifying contaminant exposure. The primary objective of this research was to implement a biomarker‐based method of exposure assessment using caged fish and real‐time reverse‐transcriptase polymerase chain reaction (rtRT‐PCR) measurements of gene expression. Primers were developed for the CYPIA, metallothionein, and vitellogenin genes in rainbow trout ( Oncorhynchyus mykiss ), cutbow trout ( Oncorhynchyus clarkii × mykiss ), and Atlantic salmon ( Salmo salar ). Each of these genes has been shown to respond specifically to planar aromatic compounds, heavy metals, and environmental estrogens, respectively. Juvenile fish were placed in cages and exposed in situ at reference and contaminated sites on the Cache la Poudre River (CO, USA), the Arkansas River (CO, USA), the St. John River (NB, Canada), and two urban creeks near Dayton (OH, USA). Quantitative gene expression was determined using rtRT‐PCR. Biomarker expression profiles were obtained that demonstrated differences in CYP1A, metallothionein, and vitellogenin mRNA production unique to each site, indicating that specific types of compounds were bioavailable and present in sufficient concentrations to elicit transcriptional responses in the organism. These findings support the use of a biomarker‐based approach to exposure identification and assessment. |
format |
Article in Journal/Newspaper |
author |
Roberts, Aaron P. Oris, James T. Burton, G. Allen Clements, William H. |
spellingShingle |
Roberts, Aaron P. Oris, James T. Burton, G. Allen Clements, William H. Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
author_facet |
Roberts, Aaron P. Oris, James T. Burton, G. Allen Clements, William H. |
author_sort |
Roberts, Aaron P. |
title |
Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
title_short |
Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
title_full |
Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
title_fullStr |
Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
title_full_unstemmed |
Gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
title_sort |
gene expression in caged fish as a first‐tier indicator of contaminant exposure in streams |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1897/05-137r.1 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1897%2F05-137R.1 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1897/05-137R.1 |
long_lat |
ENVELOPE(-158.683,-158.683,-85.733,-85.733) |
geographic |
Canada Dayton |
geographic_facet |
Canada Dayton |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Environmental Toxicology and Chemistry volume 24, issue 12, page 3092-3098 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1897/05-137r.1 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
24 |
container_issue |
12 |
container_start_page |
3092 |
op_container_end_page |
3098 |
_version_ |
1802642477527597056 |