Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla
Abstract In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[ a ]pyrene ([B a P], at 0, 0.1, 1, 10, and 50 mg/kg), β‐naph...
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crwiley:10.1897/02-378 2024-09-15T17:39:38+00:00 Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla Regoli, Francesco Winston, Gary W. Gorbi, Stefania Frenzilli, Giada Nigro, Marco Corsi, Ilaria Focardi, Silvano 2003 http://dx.doi.org/10.1897/02-378 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1897%2F02-378 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1897/02-378 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Toxicology and Chemistry volume 22, issue 9, page 2120-2129 ISSN 0730-7268 1552-8618 journal-article 2003 crwiley https://doi.org/10.1897/02-378 2024-09-05T05:08:47Z Abstract In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[ a ]pyrene ([B a P], at 0, 0.1, 1, 10, and 50 mg/kg), β‐naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor® 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8‐tetrachlorodibenzo p ‐dioxin ([TCDD], at 0, 0.01, 0.1, 1, and 2 μg/kg). The integral relationships were analyzed between induction of ethoxyresorufin O ‐deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (·OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive ·OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons B a P and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge ·OH was lowest. These data suggests a general relationship between oxidative stress and loss of DNA integrity in ... Article in Journal/Newspaper Anguilla anguilla European eel Wiley Online Library Environmental Toxicology and Chemistry 22 9 2120 |
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Abstract In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[ a ]pyrene ([B a P], at 0, 0.1, 1, 10, and 50 mg/kg), β‐naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor® 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8‐tetrachlorodibenzo p ‐dioxin ([TCDD], at 0, 0.01, 0.1, 1, and 2 μg/kg). The integral relationships were analyzed between induction of ethoxyresorufin O ‐deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (·OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive ·OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons B a P and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge ·OH was lowest. These data suggests a general relationship between oxidative stress and loss of DNA integrity in ... |
format |
Article in Journal/Newspaper |
author |
Regoli, Francesco Winston, Gary W. Gorbi, Stefania Frenzilli, Giada Nigro, Marco Corsi, Ilaria Focardi, Silvano |
spellingShingle |
Regoli, Francesco Winston, Gary W. Gorbi, Stefania Frenzilli, Giada Nigro, Marco Corsi, Ilaria Focardi, Silvano Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
author_facet |
Regoli, Francesco Winston, Gary W. Gorbi, Stefania Frenzilli, Giada Nigro, Marco Corsi, Ilaria Focardi, Silvano |
author_sort |
Regoli, Francesco |
title |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
title_short |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
title_full |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
title_fullStr |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
title_full_unstemmed |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla |
title_sort |
integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel anguilla anguilla |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1897/02-378 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1897%2F02-378 https://setac.onlinelibrary.wiley.com/doi/pdf/10.1897/02-378 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Environmental Toxicology and Chemistry volume 22, issue 9, page 2120-2129 ISSN 0730-7268 1552-8618 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1897/02-378 |
container_title |
Environmental Toxicology and Chemistry |
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22 |
container_issue |
9 |
container_start_page |
2120 |
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1810481354628923392 |