Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon
Abstract The effect of oxytetracycline (OTC) treatment on intestinal bacterial populations in juvenile Atlantic salmon Salmo salar was evaluated. Oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. Restriction fragment length polymorphism and sequencing of 1...
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crwiley:10.1577/h07-043.1 2024-09-09T19:30:13+00:00 Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon Navarrete, Paola Mardones, Pamela Opazo, Rafael Espejo, Romilio Romero, Jaime Fondo Nacional de Desarrollo Científico y Tecnológico 2008 http://dx.doi.org/10.1577/h07-043.1 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1577/H07-043.1 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Aquatic Animal Health volume 20, issue 3, page 177-183 ISSN 0899-7659 1548-8667 journal-article 2008 crwiley https://doi.org/10.1577/h07-043.1 2024-08-01T04:20:15Z Abstract The effect of oxytetracycline (OTC) treatment on intestinal bacterial populations in juvenile Atlantic salmon Salmo salar was evaluated. Oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. Restriction fragment length polymorphism and sequencing of 16S rDNA from isolates were used to analyze the intestinal microbiota before, during, and after OTC administration. The microbiota from untreated fish was more diverse, consisting mainly of Pseudomonas , Acinetobacter , Bacillus , Flavobacterium , Psycrobacter , and Brevundimonas spp. In contrast, the microbiota of the OTC‐treated group was characterized by lower diversity and consisted only of Aeromonas , clustering with A. sobria and A. salmonicida . Antibiotic‐resistant isolates were identified as Aeromonas spp.; sequencing the resistance determinant showed it to be the tetE gene. Overall, OTC treatment changed the composition of the intestinal microbiota of Atlantic salmon, as evidenced by a reduction in bacterial diversity. These results support the current concern that antibiotic treatment can facilitate the proliferation of opportunistic bacteria by eradicating competing microorganisms. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Aquatic Animal Health 20 3 177 183 |
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Wiley Online Library |
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language |
English |
description |
Abstract The effect of oxytetracycline (OTC) treatment on intestinal bacterial populations in juvenile Atlantic salmon Salmo salar was evaluated. Oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. Restriction fragment length polymorphism and sequencing of 16S rDNA from isolates were used to analyze the intestinal microbiota before, during, and after OTC administration. The microbiota from untreated fish was more diverse, consisting mainly of Pseudomonas , Acinetobacter , Bacillus , Flavobacterium , Psycrobacter , and Brevundimonas spp. In contrast, the microbiota of the OTC‐treated group was characterized by lower diversity and consisted only of Aeromonas , clustering with A. sobria and A. salmonicida . Antibiotic‐resistant isolates were identified as Aeromonas spp.; sequencing the resistance determinant showed it to be the tetE gene. Overall, OTC treatment changed the composition of the intestinal microbiota of Atlantic salmon, as evidenced by a reduction in bacterial diversity. These results support the current concern that antibiotic treatment can facilitate the proliferation of opportunistic bacteria by eradicating competing microorganisms. |
author2 |
Fondo Nacional de Desarrollo Científico y Tecnológico |
format |
Article in Journal/Newspaper |
author |
Navarrete, Paola Mardones, Pamela Opazo, Rafael Espejo, Romilio Romero, Jaime |
spellingShingle |
Navarrete, Paola Mardones, Pamela Opazo, Rafael Espejo, Romilio Romero, Jaime Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
author_facet |
Navarrete, Paola Mardones, Pamela Opazo, Rafael Espejo, Romilio Romero, Jaime |
author_sort |
Navarrete, Paola |
title |
Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
title_short |
Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
title_full |
Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
title_fullStr |
Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
title_full_unstemmed |
Oxytetracycline Treatment Reduces Bacterial Diversity of Intestinal Microbiota of Atlantic Salmon |
title_sort |
oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of atlantic salmon |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1577/h07-043.1 https://afspubs.onlinelibrary.wiley.com/doi/pdf/10.1577/H07-043.1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Aquatic Animal Health volume 20, issue 3, page 177-183 ISSN 0899-7659 1548-8667 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1577/h07-043.1 |
container_title |
Journal of Aquatic Animal Health |
container_volume |
20 |
container_issue |
3 |
container_start_page |
177 |
op_container_end_page |
183 |
_version_ |
1809899223117725696 |