Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein‐coupled receptors. The EST sequence of CXCR4 from turbot ( Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full‐length cDNA sequence of turbot CXCR4 was obtained, and sequence...
Published in: | BioMed Research International |
---|---|
Main Authors: | , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2009
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1155/2009/767893 http://downloads.hindawi.com/journals/bmri/2009/767893.pdf http://downloads.hindawi.com/journals/bmri/2009/767893.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2009/767893 |
id |
crwiley:10.1155/2009/767893 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1155/2009/767893 2024-06-23T07:56:36+00:00 Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus Jia, Airong Zhang, Xiao-Hua Griffith, Thomas National Natural Science Foundation of China 2009 http://dx.doi.org/10.1155/2009/767893 http://downloads.hindawi.com/journals/bmri/2009/767893.pdf http://downloads.hindawi.com/journals/bmri/2009/767893.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2009/767893 en eng Wiley http://creativecommons.org/licenses/by/3.0/ BioMed Research International volume 2009, issue 1 ISSN 2314-6133 2314-6141 journal-article 2009 crwiley https://doi.org/10.1155/2009/767893 2024-06-13T04:24:38Z Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein‐coupled receptors. The EST sequence of CXCR4 from turbot ( Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full‐length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real‐time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi , the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library BioMed Research International 2009 1 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein‐coupled receptors. The EST sequence of CXCR4 from turbot ( Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full‐length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real‐time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi , the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot. |
author2 |
Griffith, Thomas National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Jia, Airong Zhang, Xiao-Hua |
spellingShingle |
Jia, Airong Zhang, Xiao-Hua Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
author_facet |
Jia, Airong Zhang, Xiao-Hua |
author_sort |
Jia, Airong |
title |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_short |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_fullStr |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_full_unstemmed |
Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus |
title_sort |
molecular cloning, characterization, and expression analysis of the cxcr4 gene from turbot: scophthalmus maximus |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1155/2009/767893 http://downloads.hindawi.com/journals/bmri/2009/767893.pdf http://downloads.hindawi.com/journals/bmri/2009/767893.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2009/767893 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
BioMed Research International volume 2009, issue 1 ISSN 2314-6133 2314-6141 |
op_rights |
http://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.1155/2009/767893 |
container_title |
BioMed Research International |
container_volume |
2009 |
container_issue |
1 |
_version_ |
1802649858054553600 |