Carbamino compounds of haemoglobin in human adult and foetal blood
1. The carbamate (HbCO 2 ) concentration in oxygenated and deoxygenated human adult and foetal red blood cells was estimated at a constant pressure of carbon dioxide ( P CO 2 = 40 mm Hg) and various pH values of the serum. The Donnan ratio for chloride and bicarbonate ions was used to calculate the...
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crwiley:10.1113/jphysiol.1972.sp010042 2024-09-09T19:36:08+00:00 Carbamino compounds of haemoglobin in human adult and foetal blood Bauer, C. Schröder, E. 1972 http://dx.doi.org/10.1113/jphysiol.1972.sp010042 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.1972.sp010042 https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.1972.sp010042 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The Journal of Physiology volume 227, issue 2, page 457-471 ISSN 0022-3751 1469-7793 journal-article 1972 crwiley https://doi.org/10.1113/jphysiol.1972.sp010042 2024-08-09T04:26:42Z 1. The carbamate (HbCO 2 ) concentration in oxygenated and deoxygenated human adult and foetal red blood cells was estimated at a constant pressure of carbon dioxide ( P CO 2 = 40 mm Hg) and various pH values of the serum. The Donnan ratio for chloride and bicarbonate ions was used to calculate the bicarbonate concentration in the red cells. With this figure the carbamate concentration was calculated as follows: [HbCO 2 ] = [Total CO 2 ] — [HCO − 3 ] — [dissolved CO 2 ]. 2. At a given pH value in the red cell deoxygenated foetal red cells contain more HbCO 2 than deoxygenated adult ones. Upon oxygenation (at constant pH) HbCO 2 drops in both types of erythrocytes to lower values than in deoxygenated cells. The fraction of ‘oxylabile carbamate’ (−ΔHbCO 2 /ΔHbO 2 ) at a red cell pH of 7·2 and a P CO 2 of 40 mm Hg is 0·117 in foetal and 0·081 in adult erythrocytes. 3. From the fraction of moles carbamate formed per Hb monomer (moles CO 2 /mole Hb i ) K ′ c and K ′ z , the apparent carbamate equilibrium constants were calculated which can be used to estimate the carbamate concentration in normal adult and foetal blood. 4. The first apparent dissociation constant of carbonic acid is significantly higher in oxygenated (−log 10 K ′ 1 = pK′ 1 = 6·10) than in deoxygenated (pK′ 1 = 6·12) adult red cells, whereas in foetal red cells the difference is smaller and statistically not significant. 5. For a given set of physiological conditions in arterial and mixed venous blood in respect to oxygen saturation, P CO 2 and pH, the fractional contribution of carbamino compounds of haemoglobin to the amount of carbon dioxide which is exchanged during the respiratory cycle was computed on the basis of the present results and found to be 10·5% in adult and 19% in foetal blood. Article in Journal/Newspaper Carbonic acid Wiley Online Library The Journal of Physiology 227 2 457 471 |
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1. The carbamate (HbCO 2 ) concentration in oxygenated and deoxygenated human adult and foetal red blood cells was estimated at a constant pressure of carbon dioxide ( P CO 2 = 40 mm Hg) and various pH values of the serum. The Donnan ratio for chloride and bicarbonate ions was used to calculate the bicarbonate concentration in the red cells. With this figure the carbamate concentration was calculated as follows: [HbCO 2 ] = [Total CO 2 ] — [HCO − 3 ] — [dissolved CO 2 ]. 2. At a given pH value in the red cell deoxygenated foetal red cells contain more HbCO 2 than deoxygenated adult ones. Upon oxygenation (at constant pH) HbCO 2 drops in both types of erythrocytes to lower values than in deoxygenated cells. The fraction of ‘oxylabile carbamate’ (−ΔHbCO 2 /ΔHbO 2 ) at a red cell pH of 7·2 and a P CO 2 of 40 mm Hg is 0·117 in foetal and 0·081 in adult erythrocytes. 3. From the fraction of moles carbamate formed per Hb monomer (moles CO 2 /mole Hb i ) K ′ c and K ′ z , the apparent carbamate equilibrium constants were calculated which can be used to estimate the carbamate concentration in normal adult and foetal blood. 4. The first apparent dissociation constant of carbonic acid is significantly higher in oxygenated (−log 10 K ′ 1 = pK′ 1 = 6·10) than in deoxygenated (pK′ 1 = 6·12) adult red cells, whereas in foetal red cells the difference is smaller and statistically not significant. 5. For a given set of physiological conditions in arterial and mixed venous blood in respect to oxygen saturation, P CO 2 and pH, the fractional contribution of carbamino compounds of haemoglobin to the amount of carbon dioxide which is exchanged during the respiratory cycle was computed on the basis of the present results and found to be 10·5% in adult and 19% in foetal blood. |
format |
Article in Journal/Newspaper |
author |
Bauer, C. Schröder, E. |
spellingShingle |
Bauer, C. Schröder, E. Carbamino compounds of haemoglobin in human adult and foetal blood |
author_facet |
Bauer, C. Schröder, E. |
author_sort |
Bauer, C. |
title |
Carbamino compounds of haemoglobin in human adult and foetal blood |
title_short |
Carbamino compounds of haemoglobin in human adult and foetal blood |
title_full |
Carbamino compounds of haemoglobin in human adult and foetal blood |
title_fullStr |
Carbamino compounds of haemoglobin in human adult and foetal blood |
title_full_unstemmed |
Carbamino compounds of haemoglobin in human adult and foetal blood |
title_sort |
carbamino compounds of haemoglobin in human adult and foetal blood |
publisher |
Wiley |
publishDate |
1972 |
url |
http://dx.doi.org/10.1113/jphysiol.1972.sp010042 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.1972.sp010042 https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.1972.sp010042 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
The Journal of Physiology volume 227, issue 2, page 457-471 ISSN 0022-3751 1469-7793 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1113/jphysiol.1972.sp010042 |
container_title |
The Journal of Physiology |
container_volume |
227 |
container_issue |
2 |
container_start_page |
457 |
op_container_end_page |
471 |
_version_ |
1809905382435323904 |