Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments

Abstract Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long‐term residual concentrations, which may in turn lead to the oc...

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Published in:Zoonoses and Public Health
Main Authors: Dominguez, Johana Elizabeth, Rosario, Lovera, Juliana, Sánchez, Redondo, Leandro Martin, Chacana, Pablo Anibal, Regino, Cavia, Fernández Miyakawa, Mariano Enrique
Other Authors: Agencia Nacional de Promoción Científica y Tecnológica, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2023
Subjects:
Online Access:http://dx.doi.org/10.1111/zph.13071
https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.13071
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spelling crwiley:10.1111/zph.13071 2024-09-15T18:32:09+00:00 Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments Dominguez, Johana Elizabeth Rosario, Lovera Juliana, Sánchez Redondo, Leandro Martin Chacana, Pablo Anibal Regino, Cavia Fernández Miyakawa, Mariano Enrique Agencia Nacional de Promoción Científica y Tecnológica Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires 2023 http://dx.doi.org/10.1111/zph.13071 https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.13071 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Zoonoses and Public Health volume 70, issue 7, page 627-635 ISSN 1863-1959 1863-2378 journal-article 2023 crwiley https://doi.org/10.1111/zph.13071 2024-08-15T04:19:20Z Abstract Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long‐term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial‐resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus ) were live‐trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr‐1 and genes encoding extended‐spectrum β‐lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi‐drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr‐1 gene, demonstrated by PCR and conjugation. In two ESBL‐producing Salmonella isolated from rats, CTX‐M‐2 genes were responsible for the observed resistance to third‐generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that ... Article in Journal/Newspaper Rattus rattus Wiley Online Library Zoonoses and Public Health 70 7 627 635
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collection Wiley Online Library
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description Abstract Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long‐term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial‐resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus ) were live‐trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr‐1 and genes encoding extended‐spectrum β‐lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi‐drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr‐1 gene, demonstrated by PCR and conjugation. In two ESBL‐producing Salmonella isolated from rats, CTX‐M‐2 genes were responsible for the observed resistance to third‐generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that ...
author2 Agencia Nacional de Promoción Científica y Tecnológica
Consejo Nacional de Investigaciones Científicas y Técnicas
Universidad de Buenos Aires
format Article in Journal/Newspaper
author Dominguez, Johana Elizabeth
Rosario, Lovera
Juliana, Sánchez
Redondo, Leandro Martin
Chacana, Pablo Anibal
Regino, Cavia
Fernández Miyakawa, Mariano Enrique
spellingShingle Dominguez, Johana Elizabeth
Rosario, Lovera
Juliana, Sánchez
Redondo, Leandro Martin
Chacana, Pablo Anibal
Regino, Cavia
Fernández Miyakawa, Mariano Enrique
Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
author_facet Dominguez, Johana Elizabeth
Rosario, Lovera
Juliana, Sánchez
Redondo, Leandro Martin
Chacana, Pablo Anibal
Regino, Cavia
Fernández Miyakawa, Mariano Enrique
author_sort Dominguez, Johana Elizabeth
title Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
title_short Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
title_full Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
title_fullStr Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
title_full_unstemmed Rats as sources of multidrug‐resistant Enterobacteriaceae in animal production environments
title_sort rats as sources of multidrug‐resistant enterobacteriaceae in animal production environments
publisher Wiley
publishDate 2023
url http://dx.doi.org/10.1111/zph.13071
https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.13071
genre Rattus rattus
genre_facet Rattus rattus
op_source Zoonoses and Public Health
volume 70, issue 7, page 627-635
ISSN 1863-1959 1863-2378
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1111/zph.13071
container_title Zoonoses and Public Health
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