Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway
Abstract Tick‐borne encephalitis virus (TBEV) is recognized as the most important zoonotic tick‐transmitted virus in Europe. TBEV is mainly transmitted to humans through bites from TBEV‐infected ticks ( Ixodes ricinus and Ixodes persulcatus ). However, alimentary infection after consumption of unpas...
Published in: | Zoonoses and Public Health |
---|---|
Main Authors: | , , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2018
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/zph.12554 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fzph.12554 https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.12554 |
id |
crwiley:10.1111/zph.12554 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/zph.12554 2024-10-13T14:06:28+00:00 Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway Paulsen, Katrine M. Stuen, Snorre das Neves, Carlos G. Suhel, Faisal Gurung, Deepa Soleng, Arnulf Stiasny, Karin Vikse, Rose Andreassen, Åshild K. Granquist, Erik G. Helse- og Omsorgsdepartementet 2018 http://dx.doi.org/10.1111/zph.12554 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fzph.12554 https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.12554 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Zoonoses and Public Health volume 66, issue 2, page 216-222 ISSN 1863-1959 1863-2378 journal-article 2018 crwiley https://doi.org/10.1111/zph.12554 2024-09-19T04:18:39Z Abstract Tick‐borne encephalitis virus (TBEV) is recognized as the most important zoonotic tick‐transmitted virus in Europe. TBEV is mainly transmitted to humans through bites from TBEV‐infected ticks ( Ixodes ricinus and Ixodes persulcatus ). However, alimentary infection after consumption of unpasteurized milk and cheese from domestic ruminants has been reported. There is little information about TBEV in ruminants in Norway. The objectives of this study were to analyse unpasteurized cow milk for TBEV RNA and to study the presence of IgG antibodies to TBEV in the same animals. A total of 112 milk and blood samples were collected from cows from five different farms spread from southern to northern Norway. The milk samples were analysed by an in‐house reverse transcription (RT) real‐time polymerase chain reaction and confirmed by pyrosequencing. Serum samples were screened by a commercial enzyme‐linked immunosorbent assay and verified by a TBEV‐specific serum neutralization test. We found TBEV RNA in unpasteurized milk collected from farms in the municipalities of Mandal, Skedsmo and Brønnøy in 5.4% of the tested animals. Specific antibodies to TBEV were only detected in Arendal, where 88.2% of the tested animals were positive. Further studies on milk containing TBEV RNA should be performed to conclude if TBEV found in unpasteurized milk in Norway is infectious, which could be of great importance in a One Health perspective. Article in Journal/Newspaper Brønnøy Northern Norway Wiley Online Library Brønnøy ENVELOPE(12.206,12.206,65.468,65.468) Norway Zoonoses and Public Health 66 2 216 222 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Tick‐borne encephalitis virus (TBEV) is recognized as the most important zoonotic tick‐transmitted virus in Europe. TBEV is mainly transmitted to humans through bites from TBEV‐infected ticks ( Ixodes ricinus and Ixodes persulcatus ). However, alimentary infection after consumption of unpasteurized milk and cheese from domestic ruminants has been reported. There is little information about TBEV in ruminants in Norway. The objectives of this study were to analyse unpasteurized cow milk for TBEV RNA and to study the presence of IgG antibodies to TBEV in the same animals. A total of 112 milk and blood samples were collected from cows from five different farms spread from southern to northern Norway. The milk samples were analysed by an in‐house reverse transcription (RT) real‐time polymerase chain reaction and confirmed by pyrosequencing. Serum samples were screened by a commercial enzyme‐linked immunosorbent assay and verified by a TBEV‐specific serum neutralization test. We found TBEV RNA in unpasteurized milk collected from farms in the municipalities of Mandal, Skedsmo and Brønnøy in 5.4% of the tested animals. Specific antibodies to TBEV were only detected in Arendal, where 88.2% of the tested animals were positive. Further studies on milk containing TBEV RNA should be performed to conclude if TBEV found in unpasteurized milk in Norway is infectious, which could be of great importance in a One Health perspective. |
author2 |
Helse- og Omsorgsdepartementet |
format |
Article in Journal/Newspaper |
author |
Paulsen, Katrine M. Stuen, Snorre das Neves, Carlos G. Suhel, Faisal Gurung, Deepa Soleng, Arnulf Stiasny, Karin Vikse, Rose Andreassen, Åshild K. Granquist, Erik G. |
spellingShingle |
Paulsen, Katrine M. Stuen, Snorre das Neves, Carlos G. Suhel, Faisal Gurung, Deepa Soleng, Arnulf Stiasny, Karin Vikse, Rose Andreassen, Åshild K. Granquist, Erik G. Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
author_facet |
Paulsen, Katrine M. Stuen, Snorre das Neves, Carlos G. Suhel, Faisal Gurung, Deepa Soleng, Arnulf Stiasny, Karin Vikse, Rose Andreassen, Åshild K. Granquist, Erik G. |
author_sort |
Paulsen, Katrine M. |
title |
Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
title_short |
Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
title_full |
Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
title_fullStr |
Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
title_full_unstemmed |
Tick‐borne encephalitis virus in cows and unpasteurized cow milk from Norway |
title_sort |
tick‐borne encephalitis virus in cows and unpasteurized cow milk from norway |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/zph.12554 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fzph.12554 https://onlinelibrary.wiley.com/doi/pdf/10.1111/zph.12554 |
long_lat |
ENVELOPE(12.206,12.206,65.468,65.468) |
geographic |
Brønnøy Norway |
geographic_facet |
Brønnøy Norway |
genre |
Brønnøy Northern Norway |
genre_facet |
Brønnøy Northern Norway |
op_source |
Zoonoses and Public Health volume 66, issue 2, page 216-222 ISSN 1863-1959 1863-2378 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/zph.12554 |
container_title |
Zoonoses and Public Health |
container_volume |
66 |
container_issue |
2 |
container_start_page |
216 |
op_container_end_page |
222 |
_version_ |
1812812615816052736 |