Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation
Abstract One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body an...
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Online Access: | http://dx.doi.org/10.1111/xen.12858 https://onlinelibrary.wiley.com/doi/pdf/10.1111/xen.12858 |
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crwiley:10.1111/xen.12858 2024-09-15T17:56:38+00:00 Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation Lange, Andreas Medugorac, Ivica Ali, Asghar Kessler, Barbara Kurome, Mayuko Zakhartchenko, Valeri Hammer, Sabine E. Hauser, Andreas Denner, Joachim Dobenecker, Britta Wess, Gerhard Tan, Paul L. J. Garkavenko, Olga Reichart, Bruno Wolf, Eckhard Kemter, Elisabeth Deutsche Forschungsgemeinschaft Bayerische Forschungsstiftung 2024 http://dx.doi.org/10.1111/xen.12858 https://onlinelibrary.wiley.com/doi/pdf/10.1111/xen.12858 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Xenotransplantation volume 31, issue 2 ISSN 0908-665X 1399-3089 journal-article 2024 crwiley https://doi.org/10.1111/xen.12858 2024-08-27T04:27:22Z Abstract One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body and organ growth, cardiac function, and genetic diversity of a colony of Auckland Island pigs established at the Center for Innovative Medical Models (CiMM), LMU Munich. Male and female Auckland Island pig kidney cells (selected to be free of porcine endogenous retrovirus C) were imported from New Zealand, and founder animals were established by somatic cell nuclear transfer (SCNT). Morphologically, Auckland Island pigs have smaller body stature compared to many domestic pig breeds, rendering their organ dimensions well‐suited for human transplantation. Furthermore, echocardiography assessments of Auckland Island pig hearts indicated normal structure and functioning across various age groups throughout the study. Single nucleotide polymorphism (SNP) analysis revealed higher runs of homozygosity (ROH) in Auckland Island pigs compared to other domestic pig breeds and demonstrated that the entire locus coding the swine leukocyte antigens (SLAs) was homozygous. Based on these findings, Auckland Island pigs represent a promising genetic background for organ xenotransplantation. Article in Journal/Newspaper Auckland Island Wiley Online Library Xenotransplantation 31 2 |
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Wiley Online Library |
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English |
description |
Abstract One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body and organ growth, cardiac function, and genetic diversity of a colony of Auckland Island pigs established at the Center for Innovative Medical Models (CiMM), LMU Munich. Male and female Auckland Island pig kidney cells (selected to be free of porcine endogenous retrovirus C) were imported from New Zealand, and founder animals were established by somatic cell nuclear transfer (SCNT). Morphologically, Auckland Island pigs have smaller body stature compared to many domestic pig breeds, rendering their organ dimensions well‐suited for human transplantation. Furthermore, echocardiography assessments of Auckland Island pig hearts indicated normal structure and functioning across various age groups throughout the study. Single nucleotide polymorphism (SNP) analysis revealed higher runs of homozygosity (ROH) in Auckland Island pigs compared to other domestic pig breeds and demonstrated that the entire locus coding the swine leukocyte antigens (SLAs) was homozygous. Based on these findings, Auckland Island pigs represent a promising genetic background for organ xenotransplantation. |
author2 |
Deutsche Forschungsgemeinschaft Bayerische Forschungsstiftung |
format |
Article in Journal/Newspaper |
author |
Lange, Andreas Medugorac, Ivica Ali, Asghar Kessler, Barbara Kurome, Mayuko Zakhartchenko, Valeri Hammer, Sabine E. Hauser, Andreas Denner, Joachim Dobenecker, Britta Wess, Gerhard Tan, Paul L. J. Garkavenko, Olga Reichart, Bruno Wolf, Eckhard Kemter, Elisabeth |
spellingShingle |
Lange, Andreas Medugorac, Ivica Ali, Asghar Kessler, Barbara Kurome, Mayuko Zakhartchenko, Valeri Hammer, Sabine E. Hauser, Andreas Denner, Joachim Dobenecker, Britta Wess, Gerhard Tan, Paul L. J. Garkavenko, Olga Reichart, Bruno Wolf, Eckhard Kemter, Elisabeth Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
author_facet |
Lange, Andreas Medugorac, Ivica Ali, Asghar Kessler, Barbara Kurome, Mayuko Zakhartchenko, Valeri Hammer, Sabine E. Hauser, Andreas Denner, Joachim Dobenecker, Britta Wess, Gerhard Tan, Paul L. J. Garkavenko, Olga Reichart, Bruno Wolf, Eckhard Kemter, Elisabeth |
author_sort |
Lange, Andreas |
title |
Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
title_short |
Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
title_full |
Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
title_fullStr |
Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
title_full_unstemmed |
Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation |
title_sort |
genetic diversity, growth and heart function of auckland island pigs, a potential source for organ xenotransplantation |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1111/xen.12858 https://onlinelibrary.wiley.com/doi/pdf/10.1111/xen.12858 |
genre |
Auckland Island |
genre_facet |
Auckland Island |
op_source |
Xenotransplantation volume 31, issue 2 ISSN 0908-665X 1399-3089 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1111/xen.12858 |
container_title |
Xenotransplantation |
container_volume |
31 |
container_issue |
2 |
_version_ |
1810432822081486848 |