Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance
Background The discovery of a new Macrococcus canis species isolated from skin and infection sites of dogs led us to question if Macrococcus spp. are common in dogs and are resistant to antibiotics. Hypothesis/Objectives To evaluate the occurrence of Macrococcus spp. in dogs, determine antibiotic re...
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crwiley:10.1111/vde.12474 2024-09-15T18:20:16+00:00 Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance Cotting, Kerstin Strauss, Christian Rodriguez‐Campos, Sabrina Rostaher, Ana Fischer, Nina M. Roosje, Petra J. Favrot, Claude Perreten, Vincent Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 2017 http://dx.doi.org/10.1111/vde.12474 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fvde.12474 https://onlinelibrary.wiley.com/doi/pdf/10.1111/vde.12474 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Veterinary Dermatology volume 28, issue 6, page 559 ISSN 0959-4493 1365-3164 journal-article 2017 crwiley https://doi.org/10.1111/vde.12474 2024-08-09T04:26:26Z Background The discovery of a new Macrococcus canis species isolated from skin and infection sites of dogs led us to question if Macrococcus spp. are common in dogs and are resistant to antibiotics. Hypothesis/Objectives To evaluate the occurrence of Macrococcus spp. in dogs, determine antibiotic resistance profiles and genetic relationships. Animals One hundred and sixty two dogs (mainly West Highland white terriers and Newfoundland dogs) were screened for the presence of Macrococcus , including six dogs with Macrococcus infections. Methods Samples were taken from skin, ear canal and oral mucosa using swabs. Macrococci were identified by matrix‐assisted laser desorption ionization‐time of flight mass spectrometry, 16S rRNA sequencing and nuc ‐ PCR . Minimal inhibitory concentrations of 19 antibiotics were determined using broth microdilution. Resistance mechanisms were identified by microarray and sequencing of the fluoroquinolone‐determining region of gyrA and grlA . Sequence type ( ST ) was determined by multilocus sequence typing. Results Out of the 162 dogs, six harboured M. caseolyticus ( n = 6) and 13 harboured M. canis ( n = 16). Six isolates of M. canis and one of M. caseolyticus were obtained from infection sites. The 22 M. canis strains belonged to 20 different ST s and the seven M. caseolyticus strains to three ST s. Resistance to antibiotics was mostly associated with the detection of known genes, with mecB ‐mediated meticillin resistance being the most frequent. Conclusion and clinical importance This study gives some insights into the occurrence and genetic characteristics of antibiotic‐resistant Macrococcus from dogs. Presence of M. canis in infection sites and resistance to antibiotics emphasized that more attention should be paid to this novel bacteria species. Article in Journal/Newspaper Newfoundland Wiley Online Library Veterinary Dermatology 28 6 559 |
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Wiley Online Library |
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crwiley |
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English |
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Background The discovery of a new Macrococcus canis species isolated from skin and infection sites of dogs led us to question if Macrococcus spp. are common in dogs and are resistant to antibiotics. Hypothesis/Objectives To evaluate the occurrence of Macrococcus spp. in dogs, determine antibiotic resistance profiles and genetic relationships. Animals One hundred and sixty two dogs (mainly West Highland white terriers and Newfoundland dogs) were screened for the presence of Macrococcus , including six dogs with Macrococcus infections. Methods Samples were taken from skin, ear canal and oral mucosa using swabs. Macrococci were identified by matrix‐assisted laser desorption ionization‐time of flight mass spectrometry, 16S rRNA sequencing and nuc ‐ PCR . Minimal inhibitory concentrations of 19 antibiotics were determined using broth microdilution. Resistance mechanisms were identified by microarray and sequencing of the fluoroquinolone‐determining region of gyrA and grlA . Sequence type ( ST ) was determined by multilocus sequence typing. Results Out of the 162 dogs, six harboured M. caseolyticus ( n = 6) and 13 harboured M. canis ( n = 16). Six isolates of M. canis and one of M. caseolyticus were obtained from infection sites. The 22 M. canis strains belonged to 20 different ST s and the seven M. caseolyticus strains to three ST s. Resistance to antibiotics was mostly associated with the detection of known genes, with mecB ‐mediated meticillin resistance being the most frequent. Conclusion and clinical importance This study gives some insights into the occurrence and genetic characteristics of antibiotic‐resistant Macrococcus from dogs. Presence of M. canis in infection sites and resistance to antibiotics emphasized that more attention should be paid to this novel bacteria species. |
author2 |
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung |
format |
Article in Journal/Newspaper |
author |
Cotting, Kerstin Strauss, Christian Rodriguez‐Campos, Sabrina Rostaher, Ana Fischer, Nina M. Roosje, Petra J. Favrot, Claude Perreten, Vincent |
spellingShingle |
Cotting, Kerstin Strauss, Christian Rodriguez‐Campos, Sabrina Rostaher, Ana Fischer, Nina M. Roosje, Petra J. Favrot, Claude Perreten, Vincent Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
author_facet |
Cotting, Kerstin Strauss, Christian Rodriguez‐Campos, Sabrina Rostaher, Ana Fischer, Nina M. Roosje, Petra J. Favrot, Claude Perreten, Vincent |
author_sort |
Cotting, Kerstin |
title |
Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
title_short |
Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
title_full |
Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
title_fullStr |
Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
title_full_unstemmed |
Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
title_sort |
macrococcus canis and m. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/vde.12474 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fvde.12474 https://onlinelibrary.wiley.com/doi/pdf/10.1111/vde.12474 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Veterinary Dermatology volume 28, issue 6, page 559 ISSN 0959-4493 1365-3164 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/vde.12474 |
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Veterinary Dermatology |
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28 |
container_issue |
6 |
container_start_page |
559 |
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1810458635838423040 |