Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques
Background While hematologic reference intervals ( RI ) are available for multiple raptorial species of the order Accipitriformes and Falconiformes, there is a lack of valuable hematologic information in Strigiformes that can be used for diagnostic and health monitoring purposes. Objectives The obje...
Published in: | Veterinary Clinical Pathology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2015
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/vcp.12229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fvcp.12229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/vcp.12229 |
id |
crwiley:10.1111/vcp.12229 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/vcp.12229 2023-12-03T10:22:19+01:00 Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques Ammersbach, Mélanie Beaufrère, Hugues Gionet Rollick, Annick Tully, Thomas 2015 http://dx.doi.org/10.1111/vcp.12229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fvcp.12229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/vcp.12229 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Veterinary Clinical Pathology volume 44, issue 1, page 94-108 ISSN 0275-6382 1939-165X General Veterinary journal-article 2015 crwiley https://doi.org/10.1111/vcp.12229 2023-11-09T14:21:12Z Background While hematologic reference intervals ( RI ) are available for multiple raptorial species of the order Accipitriformes and Falconiformes, there is a lack of valuable hematologic information in Strigiformes that can be used for diagnostic and health monitoring purposes. Objectives The objective was to report RI in Strigiformes for hematologic variables and to assess agreement between manual cell counting techniques. Methods A multi‐center prospective study was designed to assess hematologic RI and blood cell morphology in owl species. Samples were collected from individuals representing 13 Strigiformes species, including Great Horned Owl, Snowy Owl, Eurasian Eagle Owl, Barred Owl, Great Gray Owl, Ural Owl, Northern Saw‐Whet Owls, Northern Hawk Owl, Spectacled Owl, Barn Owl, Eastern Screech Owl, Long‐Eared Owl, and Short‐Eared Owl. Red blood cell count was determined manually using a hemocytometer. White blood cell count was determined using 3 manual counting techniques: (1) phloxine B technique, (2) Natt and Herrick technique, and (3) estimation from the smear. Differential counts and blood cell morphology were determined on smears. Reference intervals were determined and agreement between methods was calculated. Results Important species‐specific differences were observed in blood cell counts and granulocyte morphology. Differences in WBC count between species did not appear to be predictable based on phylogenetic relationships. Overall, most boreal owl species exhibited a lower WBC count than other species. Important disagreements were found between different manual WBC counting techniques. Conclusions Disagreements observed between manual counting techniques suggest that technique‐specific RI should be used in Strigiformes. Article in Journal/Newspaper eurasian eagle-owl snowy owl Ural Owl Wiley Online Library (via Crossref) Veterinary Clinical Pathology 44 1 94 108 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
General Veterinary |
spellingShingle |
General Veterinary Ammersbach, Mélanie Beaufrère, Hugues Gionet Rollick, Annick Tully, Thomas Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
topic_facet |
General Veterinary |
description |
Background While hematologic reference intervals ( RI ) are available for multiple raptorial species of the order Accipitriformes and Falconiformes, there is a lack of valuable hematologic information in Strigiformes that can be used for diagnostic and health monitoring purposes. Objectives The objective was to report RI in Strigiformes for hematologic variables and to assess agreement between manual cell counting techniques. Methods A multi‐center prospective study was designed to assess hematologic RI and blood cell morphology in owl species. Samples were collected from individuals representing 13 Strigiformes species, including Great Horned Owl, Snowy Owl, Eurasian Eagle Owl, Barred Owl, Great Gray Owl, Ural Owl, Northern Saw‐Whet Owls, Northern Hawk Owl, Spectacled Owl, Barn Owl, Eastern Screech Owl, Long‐Eared Owl, and Short‐Eared Owl. Red blood cell count was determined manually using a hemocytometer. White blood cell count was determined using 3 manual counting techniques: (1) phloxine B technique, (2) Natt and Herrick technique, and (3) estimation from the smear. Differential counts and blood cell morphology were determined on smears. Reference intervals were determined and agreement between methods was calculated. Results Important species‐specific differences were observed in blood cell counts and granulocyte morphology. Differences in WBC count between species did not appear to be predictable based on phylogenetic relationships. Overall, most boreal owl species exhibited a lower WBC count than other species. Important disagreements were found between different manual WBC counting techniques. Conclusions Disagreements observed between manual counting techniques suggest that technique‐specific RI should be used in Strigiformes. |
format |
Article in Journal/Newspaper |
author |
Ammersbach, Mélanie Beaufrère, Hugues Gionet Rollick, Annick Tully, Thomas |
author_facet |
Ammersbach, Mélanie Beaufrère, Hugues Gionet Rollick, Annick Tully, Thomas |
author_sort |
Ammersbach, Mélanie |
title |
Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
title_short |
Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
title_full |
Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
title_fullStr |
Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
title_full_unstemmed |
Laboratory blood analysis in Strigiformes—Part I: hematologic reference intervals and agreement between manual blood cell counting techniques |
title_sort |
laboratory blood analysis in strigiformes—part i: hematologic reference intervals and agreement between manual blood cell counting techniques |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/vcp.12229 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fvcp.12229 https://onlinelibrary.wiley.com/doi/pdf/10.1111/vcp.12229 |
genre |
eurasian eagle-owl snowy owl Ural Owl |
genre_facet |
eurasian eagle-owl snowy owl Ural Owl |
op_source |
Veterinary Clinical Pathology volume 44, issue 1, page 94-108 ISSN 0275-6382 1939-165X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/vcp.12229 |
container_title |
Veterinary Clinical Pathology |
container_volume |
44 |
container_issue |
1 |
container_start_page |
94 |
op_container_end_page |
108 |
_version_ |
1784270235407220736 |