Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein
Summary Arabidopsis heterotrimeric G‐protein complex modulates pathogen‐associated molecular pattern‐triggered immunity ( PTI ) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ‐ ( agb1‐2 ) or Gγ‐subunits...
Published in: | The Plant Journal |
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Online Access: | http://dx.doi.org/10.1111/tpj.13660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.13660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.13660 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.13660 http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1111%2Ftpj.13660 |
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crwiley:10.1111/tpj.13660 2024-06-23T07:52:32+00:00 Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein Escudero, Viviana Jordá, Lucía Sopeña‐Torres, Sara Mélida, Hugo Miedes, Eva Muñoz‐Barrios, Antonio Swami, Sanjay Alexander, Danny McKee, Lauren S. Sánchez‐Vallet, Andrea Bulone, Vincent Jones, Alan M. Molina, Antonio Ministerio de Economía y Competitividad Centre of Excellence in Cognition and its Disorders, Australian Research Council Kungliga Tekniska Högskolan National Science Foundation International Educational Foundation 2017 http://dx.doi.org/10.1111/tpj.13660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.13660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.13660 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.13660 http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1111%2Ftpj.13660 en eng Wiley http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ The Plant Journal volume 92, issue 3, page 386-399 ISSN 0960-7412 1365-313X journal-article 2017 crwiley https://doi.org/10.1111/tpj.13660 2024-05-31T08:10:10Z Summary Arabidopsis heterotrimeric G‐protein complex modulates pathogen‐associated molecular pattern‐triggered immunity ( PTI ) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ‐ ( agb1‐2 ) or Gγ‐subunits have an altered wall composition compared with wild‐type plants. Here we performed a mutant screen to identify suppressors of agb1‐2 ( sgb ) that restore susceptibility to pathogens to wild‐type levels. Out of the four sgb mutants ( sgb10–sgb13 ) identified, sgb11 is a new mutant allele of ESKIMO 1 ( ESK 1 ), which encodes a plant‐specific polysaccharide O ‐acetyltransferase involved in xylan acetylation. Null alleles ( sgb11/esk1‐7 ) of ESK 1 restore to wild‐type levels the enhanced susceptibility of agb1‐2 to the necrotrophic fungus Plectosphaerella cucumerina BMM ( Pc BMM ), but not to the bacterium Pseudomonas syringae pv. tomato DC 3000 or to the oomycete Hyaloperonospora arabidopsidis . The enhanced resistance to Pc BMM of the agb1‐2 esk1‐7 double mutant was not the result of the re‐activation of deficient PTI responses in agb1‐2 . Alteration of cell wall xylan acetylation caused by ESK 1 impairment was accompanied by an enhanced accumulation of abscisic acid, the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan‐derived metabolites, and the accumulation of disease resistance‐related secondary metabolites and different osmolites. These esk1 ‐mediated responses counterbalance the defective PTI and Pc BMM susceptibility of agb1‐2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI ‐mediated resistance is partially compensated by the activation of specific cell‐wall‐triggered immune responses. Article in Journal/Newspaper eskimo* Wiley Online Library The Plant Journal 92 3 386 399 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Arabidopsis heterotrimeric G‐protein complex modulates pathogen‐associated molecular pattern‐triggered immunity ( PTI ) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ‐ ( agb1‐2 ) or Gγ‐subunits have an altered wall composition compared with wild‐type plants. Here we performed a mutant screen to identify suppressors of agb1‐2 ( sgb ) that restore susceptibility to pathogens to wild‐type levels. Out of the four sgb mutants ( sgb10–sgb13 ) identified, sgb11 is a new mutant allele of ESKIMO 1 ( ESK 1 ), which encodes a plant‐specific polysaccharide O ‐acetyltransferase involved in xylan acetylation. Null alleles ( sgb11/esk1‐7 ) of ESK 1 restore to wild‐type levels the enhanced susceptibility of agb1‐2 to the necrotrophic fungus Plectosphaerella cucumerina BMM ( Pc BMM ), but not to the bacterium Pseudomonas syringae pv. tomato DC 3000 or to the oomycete Hyaloperonospora arabidopsidis . The enhanced resistance to Pc BMM of the agb1‐2 esk1‐7 double mutant was not the result of the re‐activation of deficient PTI responses in agb1‐2 . Alteration of cell wall xylan acetylation caused by ESK 1 impairment was accompanied by an enhanced accumulation of abscisic acid, the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan‐derived metabolites, and the accumulation of disease resistance‐related secondary metabolites and different osmolites. These esk1 ‐mediated responses counterbalance the defective PTI and Pc BMM susceptibility of agb1‐2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI ‐mediated resistance is partially compensated by the activation of specific cell‐wall‐triggered immune responses. |
author2 |
Ministerio de Economía y Competitividad Centre of Excellence in Cognition and its Disorders, Australian Research Council Kungliga Tekniska Högskolan National Science Foundation International Educational Foundation |
format |
Article in Journal/Newspaper |
author |
Escudero, Viviana Jordá, Lucía Sopeña‐Torres, Sara Mélida, Hugo Miedes, Eva Muñoz‐Barrios, Antonio Swami, Sanjay Alexander, Danny McKee, Lauren S. Sánchez‐Vallet, Andrea Bulone, Vincent Jones, Alan M. Molina, Antonio |
spellingShingle |
Escudero, Viviana Jordá, Lucía Sopeña‐Torres, Sara Mélida, Hugo Miedes, Eva Muñoz‐Barrios, Antonio Swami, Sanjay Alexander, Danny McKee, Lauren S. Sánchez‐Vallet, Andrea Bulone, Vincent Jones, Alan M. Molina, Antonio Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
author_facet |
Escudero, Viviana Jordá, Lucía Sopeña‐Torres, Sara Mélida, Hugo Miedes, Eva Muñoz‐Barrios, Antonio Swami, Sanjay Alexander, Danny McKee, Lauren S. Sánchez‐Vallet, Andrea Bulone, Vincent Jones, Alan M. Molina, Antonio |
author_sort |
Escudero, Viviana |
title |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
title_short |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
title_full |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
title_fullStr |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
title_full_unstemmed |
Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein |
title_sort |
alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric g‐protein |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/tpj.13660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.13660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.13660 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.13660 http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1111%2Ftpj.13660 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
The Plant Journal volume 92, issue 3, page 386-399 ISSN 0960-7412 1365-313X |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/tpj.13660 |
container_title |
The Plant Journal |
container_volume |
92 |
container_issue |
3 |
container_start_page |
386 |
op_container_end_page |
399 |
_version_ |
1802643866149453824 |