Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Contents High levels of reactive oxygen species ( ROS ), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post‐thaw sper...

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Published in:Reproduction in Domestic Animals
Main Authors: Li, P, Xi, MD, Du, H, Qiao, XM, Liu, ZG, Wei, QW
Other Authors: Chinese Academy of Fishery Sciences, National Natural Science Foundation of China, Ministry of Agriculture of the People's Republic of China, Grantová Agentura České Republiky
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2017
Subjects:
Online Access:http://dx.doi.org/10.1111/rda.13103
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spelling crwiley:10.1111/rda.13103 2024-09-15T17:34:42+00:00 Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species Li, P Xi, MD Du, H Qiao, XM Liu, ZG Wei, QW Chinese Academy of Fishery Sciences National Natural Science Foundation of China Ministry of Agriculture of the People's Republic of China Grantová Agentura České Republiky 2017 http://dx.doi.org/10.1111/rda.13103 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Frda.13103 https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.13103 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Reproduction in Domestic Animals volume 53, issue 2, page 287-295 ISSN 0936-6768 1439-0531 journal-article 2017 crwiley https://doi.org/10.1111/rda.13103 2024-09-05T05:06:32Z Contents High levels of reactive oxygen species ( ROS ), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post‐thaw sperm characteristics and fertility in Acipenser dabryanus , Acipenser sinensis and Acipenser baerii . Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase ( CAT ), glutathione ( GSH ), cysteine ( NAC ), ascorbic acid ( VC ) or their paired combinations. Protective concentrations of CAT , GSH and VC in the three species were 25 U/ ml , 0.25‐0.5 mg/ ml and 0.5 mg/ ml , respectively. Cysteine showed no protective effect against ROS . The addition of CAT , GSH and VC positively affected either acrosome or membrane integrity of post‐thawed sperm in the three species, as well as spermatozoan motility in A. sinensis . The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS , and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility. Article in Journal/Newspaper Acipenser baerii Wiley Online Library Reproduction in Domestic Animals 53 2 287 295
institution Open Polar
collection Wiley Online Library
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language English
description Contents High levels of reactive oxygen species ( ROS ), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post‐thaw sperm characteristics and fertility in Acipenser dabryanus , Acipenser sinensis and Acipenser baerii . Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase ( CAT ), glutathione ( GSH ), cysteine ( NAC ), ascorbic acid ( VC ) or their paired combinations. Protective concentrations of CAT , GSH and VC in the three species were 25 U/ ml , 0.25‐0.5 mg/ ml and 0.5 mg/ ml , respectively. Cysteine showed no protective effect against ROS . The addition of CAT , GSH and VC positively affected either acrosome or membrane integrity of post‐thawed sperm in the three species, as well as spermatozoan motility in A. sinensis . The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS , and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.
author2 Chinese Academy of Fishery Sciences
National Natural Science Foundation of China
Ministry of Agriculture of the People's Republic of China
Grantová Agentura České Republiky
format Article in Journal/Newspaper
author Li, P
Xi, MD
Du, H
Qiao, XM
Liu, ZG
Wei, QW
spellingShingle Li, P
Xi, MD
Du, H
Qiao, XM
Liu, ZG
Wei, QW
Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
author_facet Li, P
Xi, MD
Du, H
Qiao, XM
Liu, ZG
Wei, QW
author_sort Li, P
title Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
title_short Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
title_full Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
title_fullStr Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
title_full_unstemmed Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
title_sort antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species
publisher Wiley
publishDate 2017
url http://dx.doi.org/10.1111/rda.13103
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Frda.13103
https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.13103
genre Acipenser baerii
genre_facet Acipenser baerii
op_source Reproduction in Domestic Animals
volume 53, issue 2, page 287-295
ISSN 0936-6768 1439-0531
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1111/rda.13103
container_title Reproduction in Domestic Animals
container_volume 53
container_issue 2
container_start_page 287
op_container_end_page 295
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