DNA Damage Induced by Late Spring Sunlight in Antarctica
Abstract Sunlight ultraviolet (UV) radiation constitutes an important environmental genotoxic agent that organisms are exposed to, as it can damage DNA directly, generating pyrimidine dimers, and indirectly, generating oxidized bases and single‐strand breaks (SSBs). These lesions can lead to mutatio...
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crwiley:10.1111/php.13307 2024-04-21T07:52:31+00:00 DNA Damage Induced by Late Spring Sunlight in Antarctica Fuentes‐León, Fabiana Peres de Oliveira, Andressa Quintero‐Ruiz, Nathalia Munford, Veridiana Satoru Kajitani, Gustavo Coimbra Brum, Antônio Schuch, André Passaglia Colepicolo, Pio Sánchez‐Lamar, Angel Menck, Carlos Frederico Martins Coordenação de Aperfeiçoamento de Pessoal de Nível Superior International Union of Biochemistry and Molecular Biology Fundação de Amparo à Pesquisa do Estado de São Paulo Conselho Nacional de Desenvolvimento Científico e Tecnológico 2020 http://dx.doi.org/10.1111/php.13307 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fphp.13307 https://onlinelibrary.wiley.com/doi/pdf/10.1111/php.13307 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/php.13307 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Photochemistry and Photobiology volume 96, issue 6, page 1215-1220 ISSN 0031-8655 1751-1097 Physical and Theoretical Chemistry General Medicine Biochemistry journal-article 2020 crwiley https://doi.org/10.1111/php.13307 2024-03-26T09:16:19Z Abstract Sunlight ultraviolet (UV) radiation constitutes an important environmental genotoxic agent that organisms are exposed to, as it can damage DNA directly, generating pyrimidine dimers, and indirectly, generating oxidized bases and single‐strand breaks (SSBs). These lesions can lead to mutations, triggering skin and eye disorders, including carcinogenesis and photoaging. Stratospheric ozone layer depletion, particularly in the Antarctic continent, predicts an uncertain scenario of UV incidence on the Earth in the next decades. This research evaluates the DNA damage caused by environmental exposure to late spring sunlight in the Antarctic Peninsula, where the ozone layer hole is more pronounced. These experiments were performed at the Brazilian Comandante Ferraz Antarctic Station, at King’s George Island, South Shetlands Islands. For comparison, tropical regions were also analyzed. Samples of plasmid DNA were exposed to sunlight. Cyclobutane pyrimidine dimers (CPDs), oxidized base damage and SSBs were detected using specific enzymes. In addition, an immunological approach was used to detect CPDs. The results reveal high levels of DNA damage induced by exposure under the Antarctic sunlight, inversely correlated with ozone layer thickness, confirming the high impact of ozone layer depletion on the DNA damaging action of sunlight in Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Wiley Online Library Photochemistry and Photobiology 96 6 1215 1220 |
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English |
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Physical and Theoretical Chemistry General Medicine Biochemistry |
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Physical and Theoretical Chemistry General Medicine Biochemistry Fuentes‐León, Fabiana Peres de Oliveira, Andressa Quintero‐Ruiz, Nathalia Munford, Veridiana Satoru Kajitani, Gustavo Coimbra Brum, Antônio Schuch, André Passaglia Colepicolo, Pio Sánchez‐Lamar, Angel Menck, Carlos Frederico Martins DNA Damage Induced by Late Spring Sunlight in Antarctica |
topic_facet |
Physical and Theoretical Chemistry General Medicine Biochemistry |
description |
Abstract Sunlight ultraviolet (UV) radiation constitutes an important environmental genotoxic agent that organisms are exposed to, as it can damage DNA directly, generating pyrimidine dimers, and indirectly, generating oxidized bases and single‐strand breaks (SSBs). These lesions can lead to mutations, triggering skin and eye disorders, including carcinogenesis and photoaging. Stratospheric ozone layer depletion, particularly in the Antarctic continent, predicts an uncertain scenario of UV incidence on the Earth in the next decades. This research evaluates the DNA damage caused by environmental exposure to late spring sunlight in the Antarctic Peninsula, where the ozone layer hole is more pronounced. These experiments were performed at the Brazilian Comandante Ferraz Antarctic Station, at King’s George Island, South Shetlands Islands. For comparison, tropical regions were also analyzed. Samples of plasmid DNA were exposed to sunlight. Cyclobutane pyrimidine dimers (CPDs), oxidized base damage and SSBs were detected using specific enzymes. In addition, an immunological approach was used to detect CPDs. The results reveal high levels of DNA damage induced by exposure under the Antarctic sunlight, inversely correlated with ozone layer thickness, confirming the high impact of ozone layer depletion on the DNA damaging action of sunlight in Antarctica. |
author2 |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior International Union of Biochemistry and Molecular Biology Fundação de Amparo à Pesquisa do Estado de São Paulo Conselho Nacional de Desenvolvimento Científico e Tecnológico |
format |
Article in Journal/Newspaper |
author |
Fuentes‐León, Fabiana Peres de Oliveira, Andressa Quintero‐Ruiz, Nathalia Munford, Veridiana Satoru Kajitani, Gustavo Coimbra Brum, Antônio Schuch, André Passaglia Colepicolo, Pio Sánchez‐Lamar, Angel Menck, Carlos Frederico Martins |
author_facet |
Fuentes‐León, Fabiana Peres de Oliveira, Andressa Quintero‐Ruiz, Nathalia Munford, Veridiana Satoru Kajitani, Gustavo Coimbra Brum, Antônio Schuch, André Passaglia Colepicolo, Pio Sánchez‐Lamar, Angel Menck, Carlos Frederico Martins |
author_sort |
Fuentes‐León, Fabiana |
title |
DNA Damage Induced by Late Spring Sunlight in Antarctica |
title_short |
DNA Damage Induced by Late Spring Sunlight in Antarctica |
title_full |
DNA Damage Induced by Late Spring Sunlight in Antarctica |
title_fullStr |
DNA Damage Induced by Late Spring Sunlight in Antarctica |
title_full_unstemmed |
DNA Damage Induced by Late Spring Sunlight in Antarctica |
title_sort |
dna damage induced by late spring sunlight in antarctica |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/php.13307 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fphp.13307 https://onlinelibrary.wiley.com/doi/pdf/10.1111/php.13307 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/php.13307 |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
Photochemistry and Photobiology volume 96, issue 6, page 1215-1220 ISSN 0031-8655 1751-1097 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/php.13307 |
container_title |
Photochemistry and Photobiology |
container_volume |
96 |
container_issue |
6 |
container_start_page |
1215 |
op_container_end_page |
1220 |
_version_ |
1796935734024732672 |