A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241
Abstract The Antarctic green alga Chlamydomonas sp. UWO241 is an obligate psychrophile that thrives in the cold (4–6°C) but is unable to survive at temperatures ≥18°C. Little is known how exposure to heat affects its physiology or whether it mounts a heat stress response in a manner comparable to me...
Published in: | Plant, Cell & Environment |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14203 |
id |
crwiley:10.1111/pce.14203 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/pce.14203 2024-09-15T17:45:46+00:00 A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 Cvetkovska, Marina Zhang, Xi Vakulenko, Galyna Benzaquen, Samuel Szyszka‐Mroz, Beth Malczewski, Nina Smith, David R. Hüner, Norman P.A. Canada Foundation for Innovation Canada Research Chairs Natural Sciences and Engineering Research Council of Canada 2021 http://dx.doi.org/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14203 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Plant, Cell & Environment volume 45, issue 1, page 156-177 ISSN 0140-7791 1365-3040 journal-article 2021 crwiley https://doi.org/10.1111/pce.14203 2024-07-25T04:18:59Z Abstract The Antarctic green alga Chlamydomonas sp. UWO241 is an obligate psychrophile that thrives in the cold (4–6°C) but is unable to survive at temperatures ≥18°C. Little is known how exposure to heat affects its physiology or whether it mounts a heat stress response in a manner comparable to mesophiles. Here, we dissect the responses of UWO241 to temperature stress by examining its growth, primary metabolome and transcriptome under steady‐state low temperature and heat stress conditions. In comparison with Chlamydomonas reinhardtii , UWO241 constitutively accumulates metabolites and proteins commonly considered as stress markers, including soluble sugars, antioxidants, polyamines, and heat shock proteins to ensure efficient protein folding at low temperatures. We propose that this results from life at extreme conditions. A shift from 4°C to a non‐permissive temperature of 24°C alters the UWO241 primary metabolome and transcriptome, but growth of UWO241 at higher permissive temperatures (10 and 15°C) does not provide enhanced heat protection. UWO241 also fails to induce the accumulation of HSPs when exposed to heat, suggesting that it has lost the ability to fine‐tune its heat stress response. Our work adds to the growing body of research on temperature stress in psychrophiles, many of which are threatened by climate change. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Plant, Cell & Environment 45 1 156 177 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract The Antarctic green alga Chlamydomonas sp. UWO241 is an obligate psychrophile that thrives in the cold (4–6°C) but is unable to survive at temperatures ≥18°C. Little is known how exposure to heat affects its physiology or whether it mounts a heat stress response in a manner comparable to mesophiles. Here, we dissect the responses of UWO241 to temperature stress by examining its growth, primary metabolome and transcriptome under steady‐state low temperature and heat stress conditions. In comparison with Chlamydomonas reinhardtii , UWO241 constitutively accumulates metabolites and proteins commonly considered as stress markers, including soluble sugars, antioxidants, polyamines, and heat shock proteins to ensure efficient protein folding at low temperatures. We propose that this results from life at extreme conditions. A shift from 4°C to a non‐permissive temperature of 24°C alters the UWO241 primary metabolome and transcriptome, but growth of UWO241 at higher permissive temperatures (10 and 15°C) does not provide enhanced heat protection. UWO241 also fails to induce the accumulation of HSPs when exposed to heat, suggesting that it has lost the ability to fine‐tune its heat stress response. Our work adds to the growing body of research on temperature stress in psychrophiles, many of which are threatened by climate change. |
author2 |
Canada Foundation for Innovation Canada Research Chairs Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Cvetkovska, Marina Zhang, Xi Vakulenko, Galyna Benzaquen, Samuel Szyszka‐Mroz, Beth Malczewski, Nina Smith, David R. Hüner, Norman P.A. |
spellingShingle |
Cvetkovska, Marina Zhang, Xi Vakulenko, Galyna Benzaquen, Samuel Szyszka‐Mroz, Beth Malczewski, Nina Smith, David R. Hüner, Norman P.A. A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
author_facet |
Cvetkovska, Marina Zhang, Xi Vakulenko, Galyna Benzaquen, Samuel Szyszka‐Mroz, Beth Malczewski, Nina Smith, David R. Hüner, Norman P.A. |
author_sort |
Cvetkovska, Marina |
title |
A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
title_short |
A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
title_full |
A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
title_fullStr |
A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
title_full_unstemmed |
A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonassp. UWO241 |
title_sort |
constitutive stress response is a result of low temperature growth in the antarctic green alga chlamydomonassp. uwo241 |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14203 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14203 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Plant, Cell & Environment volume 45, issue 1, page 156-177 ISSN 0140-7791 1365-3040 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/pce.14203 |
container_title |
Plant, Cell & Environment |
container_volume |
45 |
container_issue |
1 |
container_start_page |
156 |
op_container_end_page |
177 |
_version_ |
1810493666169454592 |