Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems
Summary Biological nitrogen fixation ( BNF ) performed by moss‐associated cyanobacteria is one of the main sources of new nitrogen (N) input in pristine, high‐latitude ecosystems. Yet, the nutrients that limit BNF remain elusive. Here, we tested whether this important ecosystem function is limited b...
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Online Access: | http://dx.doi.org/10.1111/nph.14331 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.14331 https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.14331 https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 |
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crwiley:10.1111/nph.14331 2024-09-30T14:44:25+00:00 Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems Rousk, Kathrin Degboe, Jefferson Michelsen, Anders Bradley, Robert Bellenger, Jean‐Philippe Det Frie Forskningsråd Danmarks Grundforskningsfond Natural Sciences and Engineering Research Council of Canada Det Frie Forskningsråd FP7 Marie Curie Actions - COFUND Danmarks Grundforskningsfond Canadian Research Chair in Boreal Biogeochemistry Natural Sciences and Engineering Research Council of Canada 2016 http://dx.doi.org/10.1111/nph.14331 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.14331 https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.14331 https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor New Phytologist volume 214, issue 1, page 97-107 ISSN 0028-646X 1469-8137 journal-article 2016 crwiley https://doi.org/10.1111/nph.14331 2024-09-05T05:06:12Z Summary Biological nitrogen fixation ( BNF ) performed by moss‐associated cyanobacteria is one of the main sources of new nitrogen (N) input in pristine, high‐latitude ecosystems. Yet, the nutrients that limit BNF remain elusive. Here, we tested whether this important ecosystem function is limited by the availability of molybdenum (Mo), phosphorus (P), or both. BNF in dominant mosses was measured with the acetylene reduction assay ( ARA ) at different time intervals following Mo and P additions, in both laboratory microcosms with mosses from a boreal spruce forest and field plots in subarctic tundra. We further used a 15 N 2 tracer technique to assess the ARA to N 2 fixation conversion ratios at our subarctic site. BNF was up to four‐fold higher shortly after the addition of Mo, in both the laboratory and field experiments. A similar positive response to Mo was found in moss colonizing cyanobacterial biomass. As the growing season progressed, nitrogenase activity became progressively more P limited. The ARA : 15 N 2 ratios increased with increasing Mo additions. These findings show that N 2 fixation activity as well as cyanobacterial biomass in dominant feather mosses from boreal forests and subarctic tundra are limited by Mo availability. Article in Journal/Newspaper Subarctic Tundra Wiley Online Library New Phytologist 214 1 97 107 |
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English |
description |
Summary Biological nitrogen fixation ( BNF ) performed by moss‐associated cyanobacteria is one of the main sources of new nitrogen (N) input in pristine, high‐latitude ecosystems. Yet, the nutrients that limit BNF remain elusive. Here, we tested whether this important ecosystem function is limited by the availability of molybdenum (Mo), phosphorus (P), or both. BNF in dominant mosses was measured with the acetylene reduction assay ( ARA ) at different time intervals following Mo and P additions, in both laboratory microcosms with mosses from a boreal spruce forest and field plots in subarctic tundra. We further used a 15 N 2 tracer technique to assess the ARA to N 2 fixation conversion ratios at our subarctic site. BNF was up to four‐fold higher shortly after the addition of Mo, in both the laboratory and field experiments. A similar positive response to Mo was found in moss colonizing cyanobacterial biomass. As the growing season progressed, nitrogenase activity became progressively more P limited. The ARA : 15 N 2 ratios increased with increasing Mo additions. These findings show that N 2 fixation activity as well as cyanobacterial biomass in dominant feather mosses from boreal forests and subarctic tundra are limited by Mo availability. |
author2 |
Det Frie Forskningsråd Danmarks Grundforskningsfond Natural Sciences and Engineering Research Council of Canada Det Frie Forskningsråd FP7 Marie Curie Actions - COFUND Danmarks Grundforskningsfond Canadian Research Chair in Boreal Biogeochemistry Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Rousk, Kathrin Degboe, Jefferson Michelsen, Anders Bradley, Robert Bellenger, Jean‐Philippe |
spellingShingle |
Rousk, Kathrin Degboe, Jefferson Michelsen, Anders Bradley, Robert Bellenger, Jean‐Philippe Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
author_facet |
Rousk, Kathrin Degboe, Jefferson Michelsen, Anders Bradley, Robert Bellenger, Jean‐Philippe |
author_sort |
Rousk, Kathrin |
title |
Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
title_short |
Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
title_full |
Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
title_fullStr |
Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
title_full_unstemmed |
Molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
title_sort |
molybdenum and phosphorus limitation of moss‐associated nitrogen fixation in boreal ecosystems |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/nph.14331 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.14331 https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.14331 https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14331 |
genre |
Subarctic Tundra |
genre_facet |
Subarctic Tundra |
op_source |
New Phytologist volume 214, issue 1, page 97-107 ISSN 0028-646X 1469-8137 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/nph.14331 |
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New Phytologist |
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214 |
container_issue |
1 |
container_start_page |
97 |
op_container_end_page |
107 |
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1811645709838974976 |