Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis
Abstract Aspartic acid racemization is a useful tool for estimating age in marine mammals; however, acid hydrolysis during sample preparation may increase the D ‐ to L ‐enantiomer ratio. To improve age estimation in Antarctic minke whale ( Balaenoptera bonaerensis ), we optimized the quantification...
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Online Access: | http://dx.doi.org/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12977 |
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crwiley:10.1111/mms.12977 2023-12-03T10:14:05+01:00 Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis Yasunaga, Genta Inoue, Satoko Bando, Takeharu Hakamada, Takashi Fujise, Yoshihiro 2022 http://dx.doi.org/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12977 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Marine Mammal Science volume 39, issue 2, page 368-386 ISSN 0824-0469 1748-7692 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2022 crwiley https://doi.org/10.1111/mms.12977 2023-11-09T13:53:57Z Abstract Aspartic acid racemization is a useful tool for estimating age in marine mammals; however, acid hydrolysis during sample preparation may increase the D ‐ to L ‐enantiomer ratio. To improve age estimation in Antarctic minke whale ( Balaenoptera bonaerensis ), we optimized the quantification of aspartic acid enantiomers in ocular lens by using ultraperformance liquid chromatography–tandem mass spectrometry combined with sample preparation by hydrolysis with deuterium chloride. Using this approach, we determined D / L in fetal and adult whale lens, examined the degree of racemization induced by acid hydrolysis, and constructed equations for age estimation. In the fetal lens, D / L obtained after hydrolysis with HCl or DCl were positively correlated with gestation age. Estimates of D / L at birth using a DCl‐hydrolysis model were much lower than those obtained using a HCl‐hydrolysis model, indicating that racemization during hydrolysis with HCl reduces the precision of subsequently derived age estimates. Furthermore, the D / L 0 unaffected by Asp‐ D generated during hydrolysis was estimated. The log e [(1 + D / L )/(1 − D / L )] value was exponentially increased with increasing earplug‐derived age, and the standard error of the age estimates after hydrolysis with DCl was lower than that after HCl. We conclude that our method can provide precise age estimates without methodological bias. Article in Journal/Newspaper Antarc* Antarctic Antarctic Minke whale Balaenoptera bonaerensis minke whale Wiley Online Library (via Crossref) Antarctic Marine Mammal Science 39 2 368 386 |
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Open Polar |
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Wiley Online Library (via Crossref) |
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crwiley |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Yasunaga, Genta Inoue, Satoko Bando, Takeharu Hakamada, Takashi Fujise, Yoshihiro Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Abstract Aspartic acid racemization is a useful tool for estimating age in marine mammals; however, acid hydrolysis during sample preparation may increase the D ‐ to L ‐enantiomer ratio. To improve age estimation in Antarctic minke whale ( Balaenoptera bonaerensis ), we optimized the quantification of aspartic acid enantiomers in ocular lens by using ultraperformance liquid chromatography–tandem mass spectrometry combined with sample preparation by hydrolysis with deuterium chloride. Using this approach, we determined D / L in fetal and adult whale lens, examined the degree of racemization induced by acid hydrolysis, and constructed equations for age estimation. In the fetal lens, D / L obtained after hydrolysis with HCl or DCl were positively correlated with gestation age. Estimates of D / L at birth using a DCl‐hydrolysis model were much lower than those obtained using a HCl‐hydrolysis model, indicating that racemization during hydrolysis with HCl reduces the precision of subsequently derived age estimates. Furthermore, the D / L 0 unaffected by Asp‐ D generated during hydrolysis was estimated. The log e [(1 + D / L )/(1 − D / L )] value was exponentially increased with increasing earplug‐derived age, and the standard error of the age estimates after hydrolysis with DCl was lower than that after HCl. We conclude that our method can provide precise age estimates without methodological bias. |
format |
Article in Journal/Newspaper |
author |
Yasunaga, Genta Inoue, Satoko Bando, Takeharu Hakamada, Takashi Fujise, Yoshihiro |
author_facet |
Yasunaga, Genta Inoue, Satoko Bando, Takeharu Hakamada, Takashi Fujise, Yoshihiro |
author_sort |
Yasunaga, Genta |
title |
Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
title_short |
Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
title_full |
Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
title_fullStr |
Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
title_full_unstemmed |
Aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in Antarctic minke whale Balaenoptera bonaerensis |
title_sort |
aspartic acid enantiomer quantification using ultraperformance liquid chromatography–tandem mass spectroscopy combined with deuterium‐chloride hydrolysis to improve age estimation in antarctic minke whale balaenoptera bonaerensis |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12977 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12977 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Minke whale Balaenoptera bonaerensis minke whale |
genre_facet |
Antarc* Antarctic Antarctic Minke whale Balaenoptera bonaerensis minke whale |
op_source |
Marine Mammal Science volume 39, issue 2, page 368-386 ISSN 0824-0469 1748-7692 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/mms.12977 |
container_title |
Marine Mammal Science |
container_volume |
39 |
container_issue |
2 |
container_start_page |
368 |
op_container_end_page |
386 |
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1784261148765323264 |