Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>)
Abstract Blow or respiratory vapor collection is a noninvasive technique for monitoring the physiology of cetaceans and although this technique shows promise, a major challenge of blow collection is difficulty quantifying samples due to variable amounts of seawater contamination. Here, we aimed to (...
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Online Access: | http://dx.doi.org/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12779 |
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crwiley:10.1111/mms.12779 2024-04-28T08:14:29+00:00 Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) Hudson, Justine M. Anderson, W. Gary Marcoux, Marianne ArcticNet Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Churchill Northern Studies Centre Fisheries and Oceans Canada University of Manitoba 2021 http://dx.doi.org/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12779 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Marine Mammal Science volume 37, issue 3, page 888-900 ISSN 0824-0469 1748-7692 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2021 crwiley https://doi.org/10.1111/mms.12779 2024-04-08T06:51:21Z Abstract Blow or respiratory vapor collection is a noninvasive technique for monitoring the physiology of cetaceans and although this technique shows promise, a major challenge of blow collection is difficulty quantifying samples due to variable amounts of seawater contamination. Here, we aimed to (1) determine whether blow samples could be collected from free‐swimming belugas, (2) assess whether urea could be used as a dilution marker to normalize blow samples, and (3) determine which factors influenced cortisol and urea concentrations. We collected a total of 252 blow samples from free‐swimming belugas in the Churchill River in Manitoba, Canada. Cortisol, an indicator of individual stress and health, was detected in 65.87% of samples with concentrations ranging from 6.73 to 963.17 pg/ml of extract volume, while urea was detected in 90.48% of samples with concentrations ranging from 0.21 to 20.30 mg/L. We were unable to verify the efficacy of urea as a dilution marker to normalize blow samples from free‐swimming belugas; however, absolute cortisol concentrations varied based on sample device and quantity rating. Although we demonstrated that blow can be successfully collected from free‐swimming belugas, further refinement of this technique is needed before it can be used as a reliable method for physiological assessments. Article in Journal/Newspaper Beluga Beluga* Churchill Churchill River Delphinapterus leucas Wiley Online Library Marine Mammal Science 37 3 888 900 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Hudson, Justine M. Anderson, W. Gary Marcoux, Marianne Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Abstract Blow or respiratory vapor collection is a noninvasive technique for monitoring the physiology of cetaceans and although this technique shows promise, a major challenge of blow collection is difficulty quantifying samples due to variable amounts of seawater contamination. Here, we aimed to (1) determine whether blow samples could be collected from free‐swimming belugas, (2) assess whether urea could be used as a dilution marker to normalize blow samples, and (3) determine which factors influenced cortisol and urea concentrations. We collected a total of 252 blow samples from free‐swimming belugas in the Churchill River in Manitoba, Canada. Cortisol, an indicator of individual stress and health, was detected in 65.87% of samples with concentrations ranging from 6.73 to 963.17 pg/ml of extract volume, while urea was detected in 90.48% of samples with concentrations ranging from 0.21 to 20.30 mg/L. We were unable to verify the efficacy of urea as a dilution marker to normalize blow samples from free‐swimming belugas; however, absolute cortisol concentrations varied based on sample device and quantity rating. Although we demonstrated that blow can be successfully collected from free‐swimming belugas, further refinement of this technique is needed before it can be used as a reliable method for physiological assessments. |
author2 |
ArcticNet Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Churchill Northern Studies Centre Fisheries and Oceans Canada University of Manitoba |
format |
Article in Journal/Newspaper |
author |
Hudson, Justine M. Anderson, W. Gary Marcoux, Marianne |
author_facet |
Hudson, Justine M. Anderson, W. Gary Marcoux, Marianne |
author_sort |
Hudson, Justine M. |
title |
Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
title_short |
Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
title_full |
Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
title_fullStr |
Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
title_full_unstemmed |
Measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> Delphinapterus leucas </scp>) |
title_sort |
measurement of cortisol in blow samples collected from free‐swimming beluga whales (<scp> delphinapterus leucas </scp>) |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mms.12779 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mms.12779 |
genre |
Beluga Beluga* Churchill Churchill River Delphinapterus leucas |
genre_facet |
Beluga Beluga* Churchill Churchill River Delphinapterus leucas |
op_source |
Marine Mammal Science volume 37, issue 3, page 888-900 ISSN 0824-0469 1748-7692 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/mms.12779 |
container_title |
Marine Mammal Science |
container_volume |
37 |
container_issue |
3 |
container_start_page |
888 |
op_container_end_page |
900 |
_version_ |
1797580521916596224 |