Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W
Summary Chromosomal damage was detected previously in the recBCD mutants of the A ntarctic bacterium P seudomonas syringae Lz 4 W , which accumulated linear chromosomal DNA leading to cell death and growth inhibition at 4° C . RecBCD protein generally repairs DNA double‐strand breaks by RecA ‐depend...
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crwiley:10.1111/mmi.12315 2024-09-30T14:24:15+00:00 Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W Sinha, Anurag K. Pavankumar, Theetha L. Kamisetty, Srinivasulu Mittal, Pragya Ray, Malay K. 2013 http://dx.doi.org/10.1111/mmi.12315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmmi.12315 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.12315 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Microbiology volume 89, issue 4, page 792-810 ISSN 0950-382X 1365-2958 journal-article 2013 crwiley https://doi.org/10.1111/mmi.12315 2024-09-17T04:52:03Z Summary Chromosomal damage was detected previously in the recBCD mutants of the A ntarctic bacterium P seudomonas syringae Lz 4 W , which accumulated linear chromosomal DNA leading to cell death and growth inhibition at 4° C . RecBCD protein generally repairs DNA double‐strand breaks by RecA ‐dependent homologous recombination pathway. Here we show that Δ recA mutant of P . syringae is not cold‐sensitive. Significantly, inactivation of additional DNA repair genes ruvAB rescued the cold‐sensitive phenotype of Δ recBCD mutant. The Δ recA and Δ ruvAB mutants were UV ‐sensitive as expected. We propose that, at low temperature DNA replication encounters barriers leading to frequent replication fork ( RF ) arrest and fork reversal. RuvAB binds to the reversed RFs ( RRFs ) having H olliday junction‐like structures and resolves them upon association with RuvC nuclease to cause linearization of the chromosome, a threat to cell survival. RecBCD prevents this by degrading the RRFs , and facilitates replication re‐initiation. This model is consistent with our observation that low temperature‐induced DNA lesions do not evoke SOS response in P . syringae . Additional studies show that two other repair genes, radA (encoding a RecA paralogue) and recF are not involved in providing cold resistance to the A ntarctic bacterium. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic Molecular Microbiology 89 4 792 810 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary Chromosomal damage was detected previously in the recBCD mutants of the A ntarctic bacterium P seudomonas syringae Lz 4 W , which accumulated linear chromosomal DNA leading to cell death and growth inhibition at 4° C . RecBCD protein generally repairs DNA double‐strand breaks by RecA ‐dependent homologous recombination pathway. Here we show that Δ recA mutant of P . syringae is not cold‐sensitive. Significantly, inactivation of additional DNA repair genes ruvAB rescued the cold‐sensitive phenotype of Δ recBCD mutant. The Δ recA and Δ ruvAB mutants were UV ‐sensitive as expected. We propose that, at low temperature DNA replication encounters barriers leading to frequent replication fork ( RF ) arrest and fork reversal. RuvAB binds to the reversed RFs ( RRFs ) having H olliday junction‐like structures and resolves them upon association with RuvC nuclease to cause linearization of the chromosome, a threat to cell survival. RecBCD prevents this by degrading the RRFs , and facilitates replication re‐initiation. This model is consistent with our observation that low temperature‐induced DNA lesions do not evoke SOS response in P . syringae . Additional studies show that two other repair genes, radA (encoding a RecA paralogue) and recF are not involved in providing cold resistance to the A ntarctic bacterium. |
format |
Article in Journal/Newspaper |
author |
Sinha, Anurag K. Pavankumar, Theetha L. Kamisetty, Srinivasulu Mittal, Pragya Ray, Malay K. |
spellingShingle |
Sinha, Anurag K. Pavankumar, Theetha L. Kamisetty, Srinivasulu Mittal, Pragya Ray, Malay K. Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
author_facet |
Sinha, Anurag K. Pavankumar, Theetha L. Kamisetty, Srinivasulu Mittal, Pragya Ray, Malay K. |
author_sort |
Sinha, Anurag K. |
title |
Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
title_short |
Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
title_full |
Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
title_fullStr |
Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
title_full_unstemmed |
Replication arrest is a major threat to growth at low temperature in Antarctic Pseudomonas syringae Lz4 W |
title_sort |
replication arrest is a major threat to growth at low temperature in antarctic pseudomonas syringae lz4 w |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/mmi.12315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmmi.12315 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.12315 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Molecular Microbiology volume 89, issue 4, page 792-810 ISSN 0950-382X 1365-2958 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/mmi.12315 |
container_title |
Molecular Microbiology |
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89 |
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4 |
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792 |
op_container_end_page |
810 |
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1811640317277896704 |