Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis
Abstract Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried...
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crwiley:10.1111/mec.14034 2024-04-28T08:34:55+00:00 Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis Kumar, Amit Castellano, Immacolata Patti, Francesco Paolo Delledonne, Massimo Abdelgawad, Hamada Beemster, Gerrit T. S. Asard, Han Palumbo, Anna Buia, Maria Cristina SZN Flagship RITMARE - The Italian Research for the Sea - Italian National Research Council Italian Ministry of Education, University and Research within the National Research Program 2017 http://dx.doi.org/10.1111/mec.14034 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.14034 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.14034 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1 http://onlinelibrary.wiley.com/termsAndConditions Molecular Ecology volume 26, issue 8, page 2276-2290 ISSN 0962-1083 1365-294X Genetics Ecology, Evolution, Behavior and Systematics journal-article 2017 crwiley https://doi.org/10.1111/mec.14034 2024-04-08T06:55:25Z Abstract Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried out on Sargassum vulgare populations growing under acidified conditions at volcanic CO 2 vents and compared with populations in a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBis CO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat‐shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes and ion homeostasis suggested that algae increased energy production to maintain ion homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signalling, information storage and processing and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO 2 vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters. Article in Journal/Newspaper Ocean acidification Wiley Online Library Molecular Ecology 26 8 2276 2290 |
institution |
Open Polar |
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Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Kumar, Amit Castellano, Immacolata Patti, Francesco Paolo Delledonne, Massimo Abdelgawad, Hamada Beemster, Gerrit T. S. Asard, Han Palumbo, Anna Buia, Maria Cristina Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics |
description |
Abstract Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a transcriptome analysis was carried out on Sargassum vulgare populations growing under acidified conditions at volcanic CO 2 vents and compared with populations in a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBis CO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat‐shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes and ion homeostasis suggested that algae increased energy production to maintain ion homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signalling, information storage and processing and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO 2 vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters. |
author2 |
SZN Flagship RITMARE - The Italian Research for the Sea - Italian National Research Council Italian Ministry of Education, University and Research within the National Research Program |
format |
Article in Journal/Newspaper |
author |
Kumar, Amit Castellano, Immacolata Patti, Francesco Paolo Delledonne, Massimo Abdelgawad, Hamada Beemster, Gerrit T. S. Asard, Han Palumbo, Anna Buia, Maria Cristina |
author_facet |
Kumar, Amit Castellano, Immacolata Patti, Francesco Paolo Delledonne, Massimo Abdelgawad, Hamada Beemster, Gerrit T. S. Asard, Han Palumbo, Anna Buia, Maria Cristina |
author_sort |
Kumar, Amit |
title |
Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
title_short |
Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
title_full |
Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
title_fullStr |
Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
title_full_unstemmed |
Molecular response of Sargassum vulgare to acidification at volcanic <scp>CO</scp> 2 vents: insights from de novo transcriptomic analysis |
title_sort |
molecular response of sargassum vulgare to acidification at volcanic <scp>co</scp> 2 vents: insights from de novo transcriptomic analysis |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/mec.14034 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.14034 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.14034 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Molecular Ecology volume 26, issue 8, page 2276-2290 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1 http://onlinelibrary.wiley.com/termsAndConditions |
op_doi |
https://doi.org/10.1111/mec.14034 |
container_title |
Molecular Ecology |
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26 |
container_issue |
8 |
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2276 |
op_container_end_page |
2290 |
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1797591435290083328 |