Gene duplication and divergence produce divergent MHC genotypes without disassortative mating
Abstract Genes of the major histocompatibility complex ( MHC ) exhibit heterozygote advantage in immune defence, which in turn can select for MHC ‐disassortative mate choice. However, many species lack this expected pattern of MHC ‐disassortative mating. A possible explanation lies in evolutionary p...
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Online Access: | http://dx.doi.org/10.1111/mec.13747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13747 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13747 |
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crwiley:10.1111/mec.13747 2024-09-15T18:28:31+00:00 Gene duplication and divergence produce divergent MHC genotypes without disassortative mating Dearborn, Donald C. Gager, Andrea B. McArthur, Andrew G. Gilmour, Morgan E. Mandzhukova, Elena Mauck, Robert A. Arthur Vining Davis Foundations National Institute of General Medical Sciences National Institutes of Health Bates College Arthur Vining Davis Foundations National Institute of General Medical Sciences National Institutes of Health Cisco Systems Canada, Inc. 2016 http://dx.doi.org/10.1111/mec.13747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13747 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13747 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 25, issue 17, page 4355-4367 ISSN 0962-1083 1365-294X journal-article 2016 crwiley https://doi.org/10.1111/mec.13747 2024-08-01T04:22:41Z Abstract Genes of the major histocompatibility complex ( MHC ) exhibit heterozygote advantage in immune defence, which in turn can select for MHC ‐disassortative mate choice. However, many species lack this expected pattern of MHC ‐disassortative mating. A possible explanation lies in evolutionary processes following gene duplication: if two duplicated MHC genes become functionally diverged from each other, offspring will inherit diverse multilocus genotypes even under random mating. We used locus‐specific primers for high‐throughput sequencing of two expressed MHC Class II B genes in Leach's storm‐petrels, Oceanodroma leucorhoa , and found that exon 2 alleles fall into two gene‐specific monophyletic clades. We tested for disassortative vs. random mating at these two functionally diverged Class II B genes, using multiple metrics and different subsets of exon 2 sequence data. With good statistical power, we consistently found random assortment of mates at MHC . Despite random mating, birds had MHC genotypes with functionally diverged alleles, averaging 13 amino acid differences in pairwise comparisons of exon 2 alleles within individuals. To test whether this high MHC diversity in individuals is driven by evolutionary divergence of the two duplicated genes, we built a phylogenetic permutation model. The model showed that genotypic diversity was strongly impacted by sequence divergence between the most common allele of each gene, with a smaller additional impact of monophyly of the two genes. Divergence of allele sequences between genes may have reduced the benefits of actively seeking MHC ‐dissimilar mates, in which case the evolutionary history of duplicated genes is shaping the adaptive landscape of sexual selection. Article in Journal/Newspaper Oceanodroma leucorhoa Wiley Online Library Molecular Ecology 25 17 4355 4367 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Genes of the major histocompatibility complex ( MHC ) exhibit heterozygote advantage in immune defence, which in turn can select for MHC ‐disassortative mate choice. However, many species lack this expected pattern of MHC ‐disassortative mating. A possible explanation lies in evolutionary processes following gene duplication: if two duplicated MHC genes become functionally diverged from each other, offspring will inherit diverse multilocus genotypes even under random mating. We used locus‐specific primers for high‐throughput sequencing of two expressed MHC Class II B genes in Leach's storm‐petrels, Oceanodroma leucorhoa , and found that exon 2 alleles fall into two gene‐specific monophyletic clades. We tested for disassortative vs. random mating at these two functionally diverged Class II B genes, using multiple metrics and different subsets of exon 2 sequence data. With good statistical power, we consistently found random assortment of mates at MHC . Despite random mating, birds had MHC genotypes with functionally diverged alleles, averaging 13 amino acid differences in pairwise comparisons of exon 2 alleles within individuals. To test whether this high MHC diversity in individuals is driven by evolutionary divergence of the two duplicated genes, we built a phylogenetic permutation model. The model showed that genotypic diversity was strongly impacted by sequence divergence between the most common allele of each gene, with a smaller additional impact of monophyly of the two genes. Divergence of allele sequences between genes may have reduced the benefits of actively seeking MHC ‐dissimilar mates, in which case the evolutionary history of duplicated genes is shaping the adaptive landscape of sexual selection. |
author2 |
Arthur Vining Davis Foundations National Institute of General Medical Sciences National Institutes of Health Bates College Arthur Vining Davis Foundations National Institute of General Medical Sciences National Institutes of Health Cisco Systems Canada, Inc. |
format |
Article in Journal/Newspaper |
author |
Dearborn, Donald C. Gager, Andrea B. McArthur, Andrew G. Gilmour, Morgan E. Mandzhukova, Elena Mauck, Robert A. |
spellingShingle |
Dearborn, Donald C. Gager, Andrea B. McArthur, Andrew G. Gilmour, Morgan E. Mandzhukova, Elena Mauck, Robert A. Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
author_facet |
Dearborn, Donald C. Gager, Andrea B. McArthur, Andrew G. Gilmour, Morgan E. Mandzhukova, Elena Mauck, Robert A. |
author_sort |
Dearborn, Donald C. |
title |
Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
title_short |
Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
title_full |
Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
title_fullStr |
Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
title_full_unstemmed |
Gene duplication and divergence produce divergent MHC genotypes without disassortative mating |
title_sort |
gene duplication and divergence produce divergent mhc genotypes without disassortative mating |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/mec.13747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13747 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13747 |
genre |
Oceanodroma leucorhoa |
genre_facet |
Oceanodroma leucorhoa |
op_source |
Molecular Ecology volume 25, issue 17, page 4355-4367 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/mec.13747 |
container_title |
Molecular Ecology |
container_volume |
25 |
container_issue |
17 |
container_start_page |
4355 |
op_container_end_page |
4367 |
_version_ |
1810469890787639296 |