New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus
Abstract Detection of population genetic structure of zooplankton at medium‐to‐small spatial scales in the absence of physical barriers has remained challenging and controversial. The large population sizes and high rates of gene flow characteristic of zooplankton have made resolution of geographica...
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crwiley:10.1111/mec.13581 2024-09-15T18:21:29+00:00 New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus Blanco‐Bercial, L. Bucklin, A. College of Liberal Arts and Sciences, University of Connecticut College of Liberal Arts and Sciences, University of Connecticut Bermuda Institute of Ocean Sciences 2016 http://dx.doi.org/10.1111/mec.13581 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13581 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13581 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 25, issue 7, page 1566-1580 ISSN 0962-1083 1365-294X journal-article 2016 crwiley https://doi.org/10.1111/mec.13581 2024-08-06T04:16:49Z Abstract Detection of population genetic structure of zooplankton at medium‐to‐small spatial scales in the absence of physical barriers has remained challenging and controversial. The large population sizes and high rates of gene flow characteristic of zooplankton have made resolution of geographical differentiation very difficult, especially when using few genetic markers and assuming equilibrium conditions. Next‐generation sequencing now allows simultaneous sampling of hundreds to thousands of genetic markers; new analytical approaches allow studies under nonequilibrium conditions and directional migration. Samples of the North Atlantic Ocean planktonic copepod, Centropages typicus , were analysed using restriction site‐associated DNA ( RAD ) sequencing on a PROTON platform. Although prior studies revealed no genetic differentiation of populations across the geographical range of the species, analysis of RAD tags showed significant structure across the North Atlantic Ocean. We also compared the likelihood for models of connectivity among NW Atlantic populations under various directional flow scenarios that replicate oceanographic conditions of the sampled domain. High‐density marker sampling with RAD sequencing markedly outperformed other technical and analytical approaches in detection of population genetic structure and characterization of connectivity of this high geneflow zooplankton species. Article in Journal/Newspaper North Atlantic Wiley Online Library Molecular Ecology 25 7 1566 1580 |
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Wiley Online Library |
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English |
description |
Abstract Detection of population genetic structure of zooplankton at medium‐to‐small spatial scales in the absence of physical barriers has remained challenging and controversial. The large population sizes and high rates of gene flow characteristic of zooplankton have made resolution of geographical differentiation very difficult, especially when using few genetic markers and assuming equilibrium conditions. Next‐generation sequencing now allows simultaneous sampling of hundreds to thousands of genetic markers; new analytical approaches allow studies under nonequilibrium conditions and directional migration. Samples of the North Atlantic Ocean planktonic copepod, Centropages typicus , were analysed using restriction site‐associated DNA ( RAD ) sequencing on a PROTON platform. Although prior studies revealed no genetic differentiation of populations across the geographical range of the species, analysis of RAD tags showed significant structure across the North Atlantic Ocean. We also compared the likelihood for models of connectivity among NW Atlantic populations under various directional flow scenarios that replicate oceanographic conditions of the sampled domain. High‐density marker sampling with RAD sequencing markedly outperformed other technical and analytical approaches in detection of population genetic structure and characterization of connectivity of this high geneflow zooplankton species. |
author2 |
College of Liberal Arts and Sciences, University of Connecticut College of Liberal Arts and Sciences, University of Connecticut Bermuda Institute of Ocean Sciences |
format |
Article in Journal/Newspaper |
author |
Blanco‐Bercial, L. Bucklin, A. |
spellingShingle |
Blanco‐Bercial, L. Bucklin, A. New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
author_facet |
Blanco‐Bercial, L. Bucklin, A. |
author_sort |
Blanco‐Bercial, L. |
title |
New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
title_short |
New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
title_full |
New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
title_fullStr |
New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
title_full_unstemmed |
New view of population genetics of zooplankton: RAD‐seq analysis reveals population structure of the North Atlantic planktonic copepod Centropages typicus |
title_sort |
new view of population genetics of zooplankton: rad‐seq analysis reveals population structure of the north atlantic planktonic copepod centropages typicus |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/mec.13581 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13581 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13581 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Molecular Ecology volume 25, issue 7, page 1566-1580 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/mec.13581 |
container_title |
Molecular Ecology |
container_volume |
25 |
container_issue |
7 |
container_start_page |
1566 |
op_container_end_page |
1580 |
_version_ |
1810460069195677696 |