Genetic subdivision and candidate genes under selection in North American grey wolves
Abstract Previous genetic studies of the highly mobile grey wolf ( Canis lupus ) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morpho...
Published in: | Molecular Ecology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2015
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/mec.13364 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13364 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13364 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13364 |
id |
crwiley:10.1111/mec.13364 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/mec.13364 2024-04-28T08:09:40+00:00 Genetic subdivision and candidate genes under selection in North American grey wolves Schweizer, Rena M. vonHoldt, Bridgett M. Harrigan, Ryan Knowles, James C. Musiani, Marco Coltman, David Novembre, John Wayne, Robert K. National Science Foundation Natural Sciences and Engineering Research Council of Canada University of Alberta University of California, Los Angeles National Science Foundation Natural Sciences and Engineering Research Council of Canada University of Alberta University of California, Los Angeles 2015 http://dx.doi.org/10.1111/mec.13364 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13364 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13364 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13364 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 25, issue 1, page 380-402 ISSN 0962-1083 1365-294X Genetics Ecology, Evolution, Behavior and Systematics journal-article 2015 crwiley https://doi.org/10.1111/mec.13364 2024-04-02T08:43:47Z Abstract Previous genetic studies of the highly mobile grey wolf ( Canis lupus ) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morphology, coat colour and metabolism. To test these predictions, we quantified population structure related to habitat using a genotyping array to assess variation in 42 036 single‐nucleotide polymorphisms (SNPs) in 111 North American grey wolves. Using these SNP data and individual‐level measurements of 12 environmental variables, we identified six ecotypes: West Forest, Boreal Forest, Arctic, High Arctic, British Columbia and Atlantic Forest. Next, we explored signals of selection across these wolf ecotypes through the use of three complementary methods to detect selection: F ST /haplotype homozygosity bivariate percentilae, bayescan , and environmentally correlated directional selection with bayenv . Across all methods, we found consistent signals of selection on genes related to morphology, coat coloration, metabolism, as predicted, as well as vision and hearing. In several high‐ranking candidate genes, including LEPR , TYR and SLC14A2 , we found variation in allele frequencies that follow environmental changes in temperature and precipitation, a result that is consistent with local adaptation rather than genetic drift. Our findings show that local adaptation can occur despite gene flow in a highly mobile species and can be detected through a moderately dense genomic scan. These patterns of local adaptation revealed by SNP genotyping likely reflect high fidelity to natal habitats of dispersing wolves, strong ecological divergence among habitats, and moderate levels of linkage in the wolf genome. Article in Journal/Newspaper Arctic Canis lupus Wiley Online Library Molecular Ecology 25 1 380 402 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Schweizer, Rena M. vonHoldt, Bridgett M. Harrigan, Ryan Knowles, James C. Musiani, Marco Coltman, David Novembre, John Wayne, Robert K. Genetic subdivision and candidate genes under selection in North American grey wolves |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics |
description |
Abstract Previous genetic studies of the highly mobile grey wolf ( Canis lupus ) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morphology, coat colour and metabolism. To test these predictions, we quantified population structure related to habitat using a genotyping array to assess variation in 42 036 single‐nucleotide polymorphisms (SNPs) in 111 North American grey wolves. Using these SNP data and individual‐level measurements of 12 environmental variables, we identified six ecotypes: West Forest, Boreal Forest, Arctic, High Arctic, British Columbia and Atlantic Forest. Next, we explored signals of selection across these wolf ecotypes through the use of three complementary methods to detect selection: F ST /haplotype homozygosity bivariate percentilae, bayescan , and environmentally correlated directional selection with bayenv . Across all methods, we found consistent signals of selection on genes related to morphology, coat coloration, metabolism, as predicted, as well as vision and hearing. In several high‐ranking candidate genes, including LEPR , TYR and SLC14A2 , we found variation in allele frequencies that follow environmental changes in temperature and precipitation, a result that is consistent with local adaptation rather than genetic drift. Our findings show that local adaptation can occur despite gene flow in a highly mobile species and can be detected through a moderately dense genomic scan. These patterns of local adaptation revealed by SNP genotyping likely reflect high fidelity to natal habitats of dispersing wolves, strong ecological divergence among habitats, and moderate levels of linkage in the wolf genome. |
author2 |
National Science Foundation Natural Sciences and Engineering Research Council of Canada University of Alberta University of California, Los Angeles National Science Foundation Natural Sciences and Engineering Research Council of Canada University of Alberta University of California, Los Angeles |
format |
Article in Journal/Newspaper |
author |
Schweizer, Rena M. vonHoldt, Bridgett M. Harrigan, Ryan Knowles, James C. Musiani, Marco Coltman, David Novembre, John Wayne, Robert K. |
author_facet |
Schweizer, Rena M. vonHoldt, Bridgett M. Harrigan, Ryan Knowles, James C. Musiani, Marco Coltman, David Novembre, John Wayne, Robert K. |
author_sort |
Schweizer, Rena M. |
title |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_short |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_full |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_fullStr |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_full_unstemmed |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_sort |
genetic subdivision and candidate genes under selection in north american grey wolves |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/mec.13364 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13364 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13364 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13364 |
genre |
Arctic Canis lupus |
genre_facet |
Arctic Canis lupus |
op_source |
Molecular Ecology volume 25, issue 1, page 380-402 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/mec.13364 |
container_title |
Molecular Ecology |
container_volume |
25 |
container_issue |
1 |
container_start_page |
380 |
op_container_end_page |
402 |
_version_ |
1797577931878301696 |