Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake

Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for thos...

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Published in:Molecular Ecology
Main Authors: López‐Bueno, A., Rastrojo, A., Peiró, R., Arenas, M., Alcamí, A.
Other Authors: Spanish Polar Programme and the Spanish Ministry of Economy and Competitiveness, Spanish Ministry of Economy and Competitiveness
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2015
Subjects:
Online Access:http://dx.doi.org/10.1111/mec.13321
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spelling crwiley:10.1111/mec.13321 2024-06-23T07:47:24+00:00 Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake López‐Bueno, A. Rastrojo, A. Peiró, R. Arenas, M. Alcamí, A. Spanish Polar Programme and the Spanish Ministry of Economy and Competitiveness Spanish Ministry of Economy and Competitiveness 2015 http://dx.doi.org/10.1111/mec.13321 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13321 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13321 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 24, issue 19, page 4812-4825 ISSN 0962-1083 1365-294X journal-article 2015 crwiley https://doi.org/10.1111/mec.13321 2024-06-11T04:46:33Z Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro‐organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low‐latitude aquatic environments, this lake harbours an RNA virome dominated by positive single‐strand RNA viruses from the order Picornavirales probably infecting micro‐organisms. Antarctic picorna‐like virus 1 ( APLV 1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low‐complexity indexes. By contrast, APLV 2‐ APLV 3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run‐off‐mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity. Article in Journal/Newspaper Antarc* Antarctic Livingston Island Wiley Online Library Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Molecular Ecology 24 19 4812 4825
institution Open Polar
collection Wiley Online Library
op_collection_id crwiley
language English
description Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro‐organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low‐latitude aquatic environments, this lake harbours an RNA virome dominated by positive single‐strand RNA viruses from the order Picornavirales probably infecting micro‐organisms. Antarctic picorna‐like virus 1 ( APLV 1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low‐complexity indexes. By contrast, APLV 2‐ APLV 3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run‐off‐mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity.
author2 Spanish Polar Programme and the Spanish Ministry of Economy and Competitiveness
Spanish Ministry of Economy and Competitiveness
format Article in Journal/Newspaper
author López‐Bueno, A.
Rastrojo, A.
Peiró, R.
Arenas, M.
Alcamí, A.
spellingShingle López‐Bueno, A.
Rastrojo, A.
Peiró, R.
Arenas, M.
Alcamí, A.
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
author_facet López‐Bueno, A.
Rastrojo, A.
Peiró, R.
Arenas, M.
Alcamí, A.
author_sort López‐Bueno, A.
title Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
title_short Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
title_full Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
title_fullStr Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
title_full_unstemmed Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
title_sort ecological connectivity shapes quasispecies structure of rna viruses in an antarctic lake
publisher Wiley
publishDate 2015
url http://dx.doi.org/10.1111/mec.13321
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13321
https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13321
long_lat ENVELOPE(-60.500,-60.500,-62.600,-62.600)
geographic Antarctic
Livingston Island
geographic_facet Antarctic
Livingston Island
genre Antarc*
Antarctic
Livingston Island
genre_facet Antarc*
Antarctic
Livingston Island
op_source Molecular Ecology
volume 24, issue 19, page 4812-4825
ISSN 0962-1083 1365-294X
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
http://doi.wiley.com/10.1002/tdm_license_1.1
op_doi https://doi.org/10.1111/mec.13321
container_title Molecular Ecology
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container_issue 19
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