Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake
Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for thos...
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crwiley:10.1111/mec.13321 2024-06-23T07:47:24+00:00 Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake López‐Bueno, A. Rastrojo, A. Peiró, R. Arenas, M. Alcamí, A. Spanish Polar Programme and the Spanish Ministry of Economy and Competitiveness Spanish Ministry of Economy and Competitiveness 2015 http://dx.doi.org/10.1111/mec.13321 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13321 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13321 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 24, issue 19, page 4812-4825 ISSN 0962-1083 1365-294X journal-article 2015 crwiley https://doi.org/10.1111/mec.13321 2024-06-11T04:46:33Z Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro‐organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low‐latitude aquatic environments, this lake harbours an RNA virome dominated by positive single‐strand RNA viruses from the order Picornavirales probably infecting micro‐organisms. Antarctic picorna‐like virus 1 ( APLV 1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low‐complexity indexes. By contrast, APLV 2‐ APLV 3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run‐off‐mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity. Article in Journal/Newspaper Antarc* Antarctic Livingston Island Wiley Online Library Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Molecular Ecology 24 19 4812 4825 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract RNA viruses exist as complex mixtures of genotypes, known as quasispecies, where the evolution potential resides in the whole community of related genotypes. Quasispecies structure and dynamics have been studied in detail for virus infecting animals and plants but remain unexplored for those infecting micro‐organisms in environmental samples. We report the first metagenomic study of RNA viruses in an Antarctic lake (Lake Limnopolar, Livingston Island). Similar to low‐latitude aquatic environments, this lake harbours an RNA virome dominated by positive single‐strand RNA viruses from the order Picornavirales probably infecting micro‐organisms. Antarctic picorna‐like virus 1 ( APLV 1), one of the most abundant viruses in the lake, does not incorporate any mutation in the consensus sequence from 2006 to 2010 and shows stable quasispecies with low‐complexity indexes. By contrast, APLV 2‐ APLV 3 are detected in the lake water exclusively in summer samples and are major constituents of surrounding cyanobacterial mats. Their quasispecies exhibit low complexity in cyanobacterial mat, but their run‐off‐mediated transfer to the lake results in a remarkable increase of complexity that may reflect the convergence of different viral quasispecies from the catchment area or replication in a more diverse host community. This is the first example of viral quasispecies from natural aquatic ecosystems and points to ecological connectivity as a modulating factor of quasispecies complexity. |
author2 |
Spanish Polar Programme and the Spanish Ministry of Economy and Competitiveness Spanish Ministry of Economy and Competitiveness |
format |
Article in Journal/Newspaper |
author |
López‐Bueno, A. Rastrojo, A. Peiró, R. Arenas, M. Alcamí, A. |
spellingShingle |
López‐Bueno, A. Rastrojo, A. Peiró, R. Arenas, M. Alcamí, A. Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
author_facet |
López‐Bueno, A. Rastrojo, A. Peiró, R. Arenas, M. Alcamí, A. |
author_sort |
López‐Bueno, A. |
title |
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_short |
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_full |
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_fullStr |
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_full_unstemmed |
Ecological connectivity shapes quasispecies structure of RNA viruses in an Antarctic lake |
title_sort |
ecological connectivity shapes quasispecies structure of rna viruses in an antarctic lake |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/mec.13321 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13321 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13321 |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Livingston Island |
geographic_facet |
Antarctic Livingston Island |
genre |
Antarc* Antarctic Livingston Island |
genre_facet |
Antarc* Antarctic Livingston Island |
op_source |
Molecular Ecology volume 24, issue 19, page 4812-4825 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/mec.13321 |
container_title |
Molecular Ecology |
container_volume |
24 |
container_issue |
19 |
container_start_page |
4812 |
op_container_end_page |
4825 |
_version_ |
1802651501561118720 |