Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing
Abstract Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few...
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Online Access: | http://dx.doi.org/10.1111/mec.13160 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13160 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13160 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13160 |
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crwiley:10.1111/mec.13160 2024-06-23T07:55:13+00:00 Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth‐Anne Edvardsen, Bente Research Council of Norway through Grant ASSEMBLE FP7 EU project BioMarKs EU-ERC 2015 http://dx.doi.org/10.1111/mec.13160 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13160 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13160 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13160 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 24, issue 12, page 3026-3042 ISSN 0962-1083 1365-294X journal-article 2015 crwiley https://doi.org/10.1111/mec.13160 2024-06-11T04:45:51Z Abstract Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high‐throughput sequencing. From outer Oslofjorden, S Norway, nano‐ and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/ cDNA with Haptophyta‐specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c . 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA ‐sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex , Emiliania huxleyi and Phaeocystis cordata , whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP‐3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in‐depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters. Article in Journal/Newspaper North Atlantic Wiley Online Library Norway Molecular Ecology 24 12 3026 3042 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high‐throughput sequencing. From outer Oslofjorden, S Norway, nano‐ and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/ cDNA with Haptophyta‐specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c . 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA ‐sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex , Emiliania huxleyi and Phaeocystis cordata , whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP‐3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in‐depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters. |
author2 |
Research Council of Norway through Grant ASSEMBLE FP7 EU project BioMarKs EU-ERC |
format |
Article in Journal/Newspaper |
author |
Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth‐Anne Edvardsen, Bente |
spellingShingle |
Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth‐Anne Edvardsen, Bente Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
author_facet |
Egge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth‐Anne Edvardsen, Bente |
author_sort |
Egge, Elianne Sirnæs |
title |
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
title_short |
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
title_full |
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
title_fullStr |
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
title_full_unstemmed |
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high‐throughput sequencing |
title_sort |
seasonal diversity and dynamics of haptophytes in the skagerrak, norway, explored by high‐throughput sequencing |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/mec.13160 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13160 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13160 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13160 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Molecular Ecology volume 24, issue 12, page 3026-3042 ISSN 0962-1083 1365-294X |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/mec.13160 |
container_title |
Molecular Ecology |
container_volume |
24 |
container_issue |
12 |
container_start_page |
3026 |
op_container_end_page |
3042 |
_version_ |
1802647716618043392 |