Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska
Abstract Understanding the response of permafrost microbial communities to climate warming is crucial for evaluating ecosystem feedbacks to global change. This study investigated soil bacterial and archaeal communities by Illumina MiSeq sequencing of 16S r RNA gene amplicons across a permafrost thaw...
Published in: | Molecular Ecology |
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Online Access: | http://dx.doi.org/10.1111/mec.13015 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13015 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/am-pdf/10.1111/mec.13015 |
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crwiley:10.1111/mec.13015 2024-09-15T18:29:44+00:00 Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska Deng, Jie Gu, Yunfu Zhang, Jin Xue, Kai Qin, Yujia Yuan, Mengting Yin, Huaqun He, Zhili Wu, Liyou Schuur, Edward A. G. Tiedje, James M. Zhou, Jizhong U.S. Department of Energy U.S. Department of Energy 2014 http://dx.doi.org/10.1111/mec.13015 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13015 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/am-pdf/10.1111/mec.13015 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology volume 24, issue 1, page 222-234 ISSN 0962-1083 1365-294X journal-article 2014 crwiley https://doi.org/10.1111/mec.13015 2024-09-05T05:07:43Z Abstract Understanding the response of permafrost microbial communities to climate warming is crucial for evaluating ecosystem feedbacks to global change. This study investigated soil bacterial and archaeal communities by Illumina MiSeq sequencing of 16S r RNA gene amplicons across a permafrost thaw gradient at different depths in Alaska with thaw progression for over three decades. Over 4.6 million passing 16S r RNA gene sequences were obtained from a total of 97 samples, corresponding to 61 known classes and 470 genera. Soil depth and the associated soil physical–chemical properties had predominant impacts on the diversity and composition of the microbial communities. Both richness and evenness of the microbial communities decreased with soil depth. Acidobacteria, Verrucomicrobia, Alpha‐ and Gamma‐Proteobacteria dominated the microbial communities in the upper horizon, whereas abundances of Bacteroidetes, Delta‐Proteobacteria and Firmicutes increased towards deeper soils. Effects of thaw progression were absent in microbial communities in the near‐surface organic soil, probably due to greater temperature variation. Thaw progression decreased the abundances of the majority of the associated taxa in the lower organic soil, but increased the abundances of those in the mineral soil, including groups potentially involved in recalcitrant C degradation (Actinomycetales, Chitinophaga , etc.). The changes in microbial communities may be related to altered soil C sources by thaw progression. Collectively, this study revealed different impacts of thaw in the organic and mineral horizons and suggests the importance of studying both the upper and deeper soils while evaluating microbial responses to permafrost thaw. Article in Journal/Newspaper permafrost Tundra Alaska Wiley Online Library Molecular Ecology 24 1 222 234 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Understanding the response of permafrost microbial communities to climate warming is crucial for evaluating ecosystem feedbacks to global change. This study investigated soil bacterial and archaeal communities by Illumina MiSeq sequencing of 16S r RNA gene amplicons across a permafrost thaw gradient at different depths in Alaska with thaw progression for over three decades. Over 4.6 million passing 16S r RNA gene sequences were obtained from a total of 97 samples, corresponding to 61 known classes and 470 genera. Soil depth and the associated soil physical–chemical properties had predominant impacts on the diversity and composition of the microbial communities. Both richness and evenness of the microbial communities decreased with soil depth. Acidobacteria, Verrucomicrobia, Alpha‐ and Gamma‐Proteobacteria dominated the microbial communities in the upper horizon, whereas abundances of Bacteroidetes, Delta‐Proteobacteria and Firmicutes increased towards deeper soils. Effects of thaw progression were absent in microbial communities in the near‐surface organic soil, probably due to greater temperature variation. Thaw progression decreased the abundances of the majority of the associated taxa in the lower organic soil, but increased the abundances of those in the mineral soil, including groups potentially involved in recalcitrant C degradation (Actinomycetales, Chitinophaga , etc.). The changes in microbial communities may be related to altered soil C sources by thaw progression. Collectively, this study revealed different impacts of thaw in the organic and mineral horizons and suggests the importance of studying both the upper and deeper soils while evaluating microbial responses to permafrost thaw. |
author2 |
U.S. Department of Energy U.S. Department of Energy |
format |
Article in Journal/Newspaper |
author |
Deng, Jie Gu, Yunfu Zhang, Jin Xue, Kai Qin, Yujia Yuan, Mengting Yin, Huaqun He, Zhili Wu, Liyou Schuur, Edward A. G. Tiedje, James M. Zhou, Jizhong |
spellingShingle |
Deng, Jie Gu, Yunfu Zhang, Jin Xue, Kai Qin, Yujia Yuan, Mengting Yin, Huaqun He, Zhili Wu, Liyou Schuur, Edward A. G. Tiedje, James M. Zhou, Jizhong Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
author_facet |
Deng, Jie Gu, Yunfu Zhang, Jin Xue, Kai Qin, Yujia Yuan, Mengting Yin, Huaqun He, Zhili Wu, Liyou Schuur, Edward A. G. Tiedje, James M. Zhou, Jizhong |
author_sort |
Deng, Jie |
title |
Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
title_short |
Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
title_full |
Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
title_fullStr |
Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
title_full_unstemmed |
Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska |
title_sort |
shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in alaska |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1111/mec.13015 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13015 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mec.13015 https://onlinelibrary.wiley.com/doi/am-pdf/10.1111/mec.13015 |
genre |
permafrost Tundra Alaska |
genre_facet |
permafrost Tundra Alaska |
op_source |
Molecular Ecology volume 24, issue 1, page 222-234 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/mec.13015 |
container_title |
Molecular Ecology |
container_volume |
24 |
container_issue |
1 |
container_start_page |
222 |
op_container_end_page |
234 |
_version_ |
1810471170096496640 |