Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus
Abstract North A tlantic rocky intertidal species have been shaped by repeated glaciations and strong latitudinal temperature gradients, making them an excellent system to study postglacial phylogeography and thermal tolerance. Population genetics data from northwestern A tlantic species, however, o...
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crwiley:10.1111/mec.12681 2024-09-15T18:23:21+00:00 Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus Chu, Nathaniel D. Kaluziak, Stefan T. Trussell, Geoffrey C. Vollmer, Steven V. National Science Foundation National Science Foundation Brown University 2014 http://dx.doi.org/10.1111/mec.12681 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.12681 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.12681 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 23, issue 7, page 1863-1873 ISSN 0962-1083 1365-294X journal-article 2014 crwiley https://doi.org/10.1111/mec.12681 2024-08-06T04:14:09Z Abstract North A tlantic rocky intertidal species have been shaped by repeated glaciations and strong latitudinal temperature gradients, making them an excellent system to study postglacial phylogeography and thermal tolerance. Population genetics data from northwestern A tlantic species, however, often show patterns inconsistent with the prediction that high dispersal should generate weaker genetic structure among populations. Here, we used next‐generation sequencing restriction‐associated DNA tags ( RAD ‐seq) and a transcriptome assembled from RNA ‐seq data to analyse the genetic structure of northwestern A tlantic populations of the low‐dispersal intertidal snail N ucella lapillus . Although previous studies in this region have detected almost no genetic structure in N . lapillus , our phylogenomic approach identified a well‐supported split between northern and southern clades. By comparing RAD ‐seq data and our transcriptome assembly, we identified thousands of fixed single‐nucleotide polymorphisms ( SNP s) between these latitudinal clades that map to protein‐coding genes, including genes associated with heat stress tolerance. These fixed SNP s might represent loci under selection for different thermal regimes in the northwestern A tlantic. Article in Journal/Newspaper North Atlantic Nucella lapillus Wiley Online Library Molecular Ecology 23 7 1863 1873 |
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crwiley |
language |
English |
description |
Abstract North A tlantic rocky intertidal species have been shaped by repeated glaciations and strong latitudinal temperature gradients, making them an excellent system to study postglacial phylogeography and thermal tolerance. Population genetics data from northwestern A tlantic species, however, often show patterns inconsistent with the prediction that high dispersal should generate weaker genetic structure among populations. Here, we used next‐generation sequencing restriction‐associated DNA tags ( RAD ‐seq) and a transcriptome assembled from RNA ‐seq data to analyse the genetic structure of northwestern A tlantic populations of the low‐dispersal intertidal snail N ucella lapillus . Although previous studies in this region have detected almost no genetic structure in N . lapillus , our phylogenomic approach identified a well‐supported split between northern and southern clades. By comparing RAD ‐seq data and our transcriptome assembly, we identified thousands of fixed single‐nucleotide polymorphisms ( SNP s) between these latitudinal clades that map to protein‐coding genes, including genes associated with heat stress tolerance. These fixed SNP s might represent loci under selection for different thermal regimes in the northwestern A tlantic. |
author2 |
National Science Foundation National Science Foundation Brown University |
format |
Article in Journal/Newspaper |
author |
Chu, Nathaniel D. Kaluziak, Stefan T. Trussell, Geoffrey C. Vollmer, Steven V. |
spellingShingle |
Chu, Nathaniel D. Kaluziak, Stefan T. Trussell, Geoffrey C. Vollmer, Steven V. Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
author_facet |
Chu, Nathaniel D. Kaluziak, Stefan T. Trussell, Geoffrey C. Vollmer, Steven V. |
author_sort |
Chu, Nathaniel D. |
title |
Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
title_short |
Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
title_full |
Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
title_fullStr |
Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
title_full_unstemmed |
Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus |
title_sort |
phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the north atlantic snail nucella lapillus |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1111/mec.12681 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.12681 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.12681 |
genre |
North Atlantic Nucella lapillus |
genre_facet |
North Atlantic Nucella lapillus |
op_source |
Molecular Ecology volume 23, issue 7, page 1863-1873 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/mec.12681 |
container_title |
Molecular Ecology |
container_volume |
23 |
container_issue |
7 |
container_start_page |
1863 |
op_container_end_page |
1873 |
_version_ |
1810463551107629056 |