Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus
Abstract Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption ( ED ) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, N...
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crwiley:10.1111/mec.12137 2024-09-15T18:41:40+00:00 Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon 2012 http://dx.doi.org/10.1111/mec.12137 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.12137 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.12137 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 Molecular Ecology volume 22, issue 6, page 1589-1608 ISSN 0962-1083 1365-294X journal-article 2012 crwiley https://doi.org/10.1111/mec.12137 2024-09-05T05:05:16Z Abstract Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption ( ED ) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin ( TBT ) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well‐known vertebrate PPAR γ ligand, to dogwhelks induced imposex in the absence of TBT . Thus, while TBT ‐induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR ‐responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. Article in Journal/Newspaper Dogwhelk Nucella lapillus Wiley Online Library Molecular Ecology 22 6 1589 1608 |
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English |
description |
Abstract Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption ( ED ) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin ( TBT ) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well‐known vertebrate PPAR γ ligand, to dogwhelks induced imposex in the absence of TBT . Thus, while TBT ‐induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR ‐responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. |
format |
Article in Journal/Newspaper |
author |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
spellingShingle |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
author_facet |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
author_sort |
Pascoal, Sonia |
title |
Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_short |
Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full |
Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_fullStr |
Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full_unstemmed |
Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_sort |
transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, nucella lapillus |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1111/mec.12137 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.12137 https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.12137 |
genre |
Dogwhelk Nucella lapillus |
genre_facet |
Dogwhelk Nucella lapillus |
op_source |
Molecular Ecology volume 22, issue 6, page 1589-1608 ISSN 0962-1083 1365-294X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor http://doi.wiley.com/10.1002/tdm_license_1.1 |
op_doi |
https://doi.org/10.1111/mec.12137 |
container_title |
Molecular Ecology |
container_volume |
22 |
container_issue |
6 |
container_start_page |
1589 |
op_container_end_page |
1608 |
_version_ |
1810486056470970368 |