Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility
Abstract Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as °C min. Hydrostatic shock experiments investigated the duration of shock usi...
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crwiley:10.1111/jwas.13018 2024-04-28T08:15:01+00:00 Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility Oliver, Luke P. Evavold, Joseph T. Cain, Kenneth D. National Institute of Food and Agriculture 2023 http://dx.doi.org/10.1111/jwas.13018 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jwas.13018 en eng Wiley http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ Journal of the World Aquaculture Society volume 54, issue 6, page 1522-1533 ISSN 0893-8849 1749-7345 Agronomy and Crop Science Aquatic Science journal-article 2023 crwiley https://doi.org/10.1111/jwas.13018 2024-04-02T08:43:36Z Abstract Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as °C min. Hydrostatic shock experiments investigated the duration of shock using 7500 or 8500 psi at 180°C min post‐fertilization. Thermal shocks investigated duration of shock and post‐fertilization shock timing using a shock of 16°C. Sterility experiments investigated egg survival when diploids were crossed with triploids. Hydrostatic shock of 7500 psi for 10 or 20°C min can induce triploidy ≥90% and exhibits survival that is statistically similar, p ≤ 0.05, to controls. Hydrostatic shock of 8500 psi for 5 or 10°C min yielded triploid induction of 93% and 100%, respectively, with survival that is statistically similar to controls, p ≤ 0.05. Thermal induction experiments indicated shocks at 120°C min post‐fertilization, for durations between 350 and 450°C min, have potential to induce triploidy ≥90% while facilitating survival statistically similar to controls, p ≤ 0.05. Induction of tetraploidy was observed. Sterility experiments determined that triploid burbot are functionally sterile. These results may allow production of burbot where sterility is required. Article in Journal/Newspaper Burbot Lota lota lota Wiley Online Library Journal of the World Aquaculture Society 54 6 1522 1533 |
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Wiley Online Library |
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crwiley |
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English |
topic |
Agronomy and Crop Science Aquatic Science |
spellingShingle |
Agronomy and Crop Science Aquatic Science Oliver, Luke P. Evavold, Joseph T. Cain, Kenneth D. Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
topic_facet |
Agronomy and Crop Science Aquatic Science |
description |
Abstract Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as °C min. Hydrostatic shock experiments investigated the duration of shock using 7500 or 8500 psi at 180°C min post‐fertilization. Thermal shocks investigated duration of shock and post‐fertilization shock timing using a shock of 16°C. Sterility experiments investigated egg survival when diploids were crossed with triploids. Hydrostatic shock of 7500 psi for 10 or 20°C min can induce triploidy ≥90% and exhibits survival that is statistically similar, p ≤ 0.05, to controls. Hydrostatic shock of 8500 psi for 5 or 10°C min yielded triploid induction of 93% and 100%, respectively, with survival that is statistically similar to controls, p ≤ 0.05. Thermal induction experiments indicated shocks at 120°C min post‐fertilization, for durations between 350 and 450°C min, have potential to induce triploidy ≥90% while facilitating survival statistically similar to controls, p ≤ 0.05. Induction of tetraploidy was observed. Sterility experiments determined that triploid burbot are functionally sterile. These results may allow production of burbot where sterility is required. |
author2 |
National Institute of Food and Agriculture |
format |
Article in Journal/Newspaper |
author |
Oliver, Luke P. Evavold, Joseph T. Cain, Kenneth D. |
author_facet |
Oliver, Luke P. Evavold, Joseph T. Cain, Kenneth D. |
author_sort |
Oliver, Luke P. |
title |
Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
title_short |
Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
title_full |
Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
title_fullStr |
Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
title_full_unstemmed |
Triploid burbot, Lota lota , production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
title_sort |
triploid burbot, lota lota , production: optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/jwas.13018 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jwas.13018 |
genre |
Burbot Lota lota lota |
genre_facet |
Burbot Lota lota lota |
op_source |
Journal of the World Aquaculture Society volume 54, issue 6, page 1522-1533 ISSN 0893-8849 1749-7345 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/jwas.13018 |
container_title |
Journal of the World Aquaculture Society |
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54 |
container_issue |
6 |
container_start_page |
1522 |
op_container_end_page |
1533 |
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1797580819893583872 |