Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica)
Abstract Crustose coralline algae (CCA) are a highly diverse group of habitat‐forming, calcifying red macroalgae (Rhodophyta) with unique adaptations to diverse irradiance regimes. A distinctive CCA phenotype adaptation, which allows them to maximize photosynthetic performance in low light, is their...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2024
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jpy.13449 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13449 |
id |
crwiley:10.1111/jpy.13449 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/jpy.13449 2024-06-23T07:46:58+00:00 Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) Montes‐Herrera, Juan C. Cimoli, Emiliano Cummings, Vonda J. D'Archino, Roberta Nelson, Wendy A. Lucieer, Arko Lucieer, Vanessa 2024 http://dx.doi.org/10.1111/jpy.13449 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13449 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Journal of Phycology volume 60, issue 3, page 695-709 ISSN 0022-3646 1529-8817 journal-article 2024 crwiley https://doi.org/10.1111/jpy.13449 2024-06-13T04:23:51Z Abstract Crustose coralline algae (CCA) are a highly diverse group of habitat‐forming, calcifying red macroalgae (Rhodophyta) with unique adaptations to diverse irradiance regimes. A distinctive CCA phenotype adaptation, which allows them to maximize photosynthetic performance in low light, is their content of a specific group of light‐harvesting pigments called phycobilins. In this study, we assessed the potential of noninvasive hyperspectral imaging (HSI) in the visible spectrum (400–800 nm) to describe the phenotypic variability in phycobilin content of an Antarctic coralline, Tethysphytum antarcticum (Hapalidiales), from two distinct locations. We validated our measurements with pigment extractions and spectrophotometry analysis, in addition to DNA barcoding using the psb A marker. Targeted spectral indices were developed and correlated with phycobilin content using linear mixed models ( R 2 = 0.64–0.7). Once applied to the HSI, the models revealed the distinct phycoerythrin spatial distribution in the two site‐specific CCA phenotypes, with thin and thick crusts, respectively. This study advances the capabilities of hyperspectral imaging as a tool to quantitatively study CCA pigmentation in relation to their phenotypic plasticity, which can be applied in laboratory studies and potentially in situ surveys using underwater hyperspectral imaging systems. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea Wiley Online Library Antarctic Ross Sea Journal of Phycology |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Crustose coralline algae (CCA) are a highly diverse group of habitat‐forming, calcifying red macroalgae (Rhodophyta) with unique adaptations to diverse irradiance regimes. A distinctive CCA phenotype adaptation, which allows them to maximize photosynthetic performance in low light, is their content of a specific group of light‐harvesting pigments called phycobilins. In this study, we assessed the potential of noninvasive hyperspectral imaging (HSI) in the visible spectrum (400–800 nm) to describe the phenotypic variability in phycobilin content of an Antarctic coralline, Tethysphytum antarcticum (Hapalidiales), from two distinct locations. We validated our measurements with pigment extractions and spectrophotometry analysis, in addition to DNA barcoding using the psb A marker. Targeted spectral indices were developed and correlated with phycobilin content using linear mixed models ( R 2 = 0.64–0.7). Once applied to the HSI, the models revealed the distinct phycoerythrin spatial distribution in the two site‐specific CCA phenotypes, with thin and thick crusts, respectively. This study advances the capabilities of hyperspectral imaging as a tool to quantitatively study CCA pigmentation in relation to their phenotypic plasticity, which can be applied in laboratory studies and potentially in situ surveys using underwater hyperspectral imaging systems. |
format |
Article in Journal/Newspaper |
author |
Montes‐Herrera, Juan C. Cimoli, Emiliano Cummings, Vonda J. D'Archino, Roberta Nelson, Wendy A. Lucieer, Arko Lucieer, Vanessa |
spellingShingle |
Montes‐Herrera, Juan C. Cimoli, Emiliano Cummings, Vonda J. D'Archino, Roberta Nelson, Wendy A. Lucieer, Arko Lucieer, Vanessa Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
author_facet |
Montes‐Herrera, Juan C. Cimoli, Emiliano Cummings, Vonda J. D'Archino, Roberta Nelson, Wendy A. Lucieer, Arko Lucieer, Vanessa |
author_sort |
Montes‐Herrera, Juan C. |
title |
Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
title_short |
Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
title_full |
Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
title_fullStr |
Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
title_full_unstemmed |
Quantifying pigment content in crustose coralline algae using hyperspectral imaging: A case study with Tethysphytum antarcticum (Ross Sea, Antarctica) |
title_sort |
quantifying pigment content in crustose coralline algae using hyperspectral imaging: a case study with tethysphytum antarcticum (ross sea, antarctica) |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1111/jpy.13449 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13449 |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_source |
Journal of Phycology volume 60, issue 3, page 695-709 ISSN 0022-3646 1529-8817 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/jpy.13449 |
container_title |
Journal of Phycology |
_version_ |
1802649689624936448 |