The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness
At high latitudes, the polar night poses a great challenge to photosynthetic organisms that must survive up to six months without light. Numerous studies have already shed light on the physiological changes involved in the acclimation of microalgae to prolonged darkness and subsequent re‐illuminatio...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2022
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.13232 |
id |
crwiley:10.1111/jpy.13232 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/jpy.13232 2024-09-30T14:41:36+00:00 The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness Sciandra, Théo Forget, Marie‐Hélène Bruyant, Flavienne Béguin, Marine Lacour, Thomas Bowler, Chris Babin, Marcel Raven, J. Fonds de recherche du Québec – Nature et technologies Human Frontier Science Program Canada Excellence Research Chairs, Government of Canada Canada First Research Excellence Fund ArcticNet 2022 http://dx.doi.org/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.13232 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 58, issue 2, page 281-296 ISSN 0022-3646 1529-8817 journal-article 2022 crwiley https://doi.org/10.1111/jpy.13232 2024-09-05T05:09:51Z At high latitudes, the polar night poses a great challenge to photosynthetic organisms that must survive up to six months without light. Numerous studies have already shed light on the physiological changes involved in the acclimation of microalgae to prolonged darkness and subsequent re‐illumination. However, these studies have never considered inter‐individual variability because they have mainly been conducted with bulk measurements. On the other hand, such long periods are likely to impact within‐population selection processes. In this study, we hypothesized that distinct subpopulations with specific traits may emerge during acclimation of a population of diatoms to darkness. We addressed this hypothesis using flow cytometry (FCM), which allow to individually characterize large numbers of cells. The ecologically dominant polar pennate diatom Fragilariopsis cylindrus was subjected to three dark acclimation (DA) experiments of one, three, and five months duration, during which all cultures showed signs of recovery once light became available again. Our results suggest that darkness survival of F. cylindrus relies on reduction of metabolic activity and consumption of carbon reserves. In addition, FCM allowed us to record three different causes of death, each shared by significant numbers of individuals. The first rendered cells were unable to survive the stress caused by the return to light, probably due to a lack of sufficient photoprotective defenses. The other two were observed in two subpopulations of cells whose physiological state deviated from the original population. The data suggest that starvation and failure to maintain dormancy were the cause of cell mortality in these two subpopulations. Article in Journal/Newspaper polar night Wiley Online Library Journal of Phycology 58 2 281 296 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
At high latitudes, the polar night poses a great challenge to photosynthetic organisms that must survive up to six months without light. Numerous studies have already shed light on the physiological changes involved in the acclimation of microalgae to prolonged darkness and subsequent re‐illumination. However, these studies have never considered inter‐individual variability because they have mainly been conducted with bulk measurements. On the other hand, such long periods are likely to impact within‐population selection processes. In this study, we hypothesized that distinct subpopulations with specific traits may emerge during acclimation of a population of diatoms to darkness. We addressed this hypothesis using flow cytometry (FCM), which allow to individually characterize large numbers of cells. The ecologically dominant polar pennate diatom Fragilariopsis cylindrus was subjected to three dark acclimation (DA) experiments of one, three, and five months duration, during which all cultures showed signs of recovery once light became available again. Our results suggest that darkness survival of F. cylindrus relies on reduction of metabolic activity and consumption of carbon reserves. In addition, FCM allowed us to record three different causes of death, each shared by significant numbers of individuals. The first rendered cells were unable to survive the stress caused by the return to light, probably due to a lack of sufficient photoprotective defenses. The other two were observed in two subpopulations of cells whose physiological state deviated from the original population. The data suggest that starvation and failure to maintain dormancy were the cause of cell mortality in these two subpopulations. |
author2 |
Raven, J. Fonds de recherche du Québec – Nature et technologies Human Frontier Science Program Canada Excellence Research Chairs, Government of Canada Canada First Research Excellence Fund ArcticNet |
format |
Article in Journal/Newspaper |
author |
Sciandra, Théo Forget, Marie‐Hélène Bruyant, Flavienne Béguin, Marine Lacour, Thomas Bowler, Chris Babin, Marcel |
spellingShingle |
Sciandra, Théo Forget, Marie‐Hélène Bruyant, Flavienne Béguin, Marine Lacour, Thomas Bowler, Chris Babin, Marcel The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
author_facet |
Sciandra, Théo Forget, Marie‐Hélène Bruyant, Flavienne Béguin, Marine Lacour, Thomas Bowler, Chris Babin, Marcel |
author_sort |
Sciandra, Théo |
title |
The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
title_short |
The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
title_full |
The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
title_fullStr |
The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
title_full_unstemmed |
The possible fates of Fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
title_sort |
possible fates of fragilariopsis cylindrus (polar diatom) cells exposed to prolonged darkness |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.13232 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.13232 |
genre |
polar night |
genre_facet |
polar night |
op_source |
Journal of Phycology volume 58, issue 2, page 281-296 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.13232 |
container_title |
Journal of Phycology |
container_volume |
58 |
container_issue |
2 |
container_start_page |
281 |
op_container_end_page |
296 |
_version_ |
1811644065266008064 |