Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1
The haptophyte Phaeocystis antarctica is endemic to the Southern Ocean, where iron supply is sporadic and its availability limits primary production. In iron fertilization experiments, P. antarctica showed a prompt and steady increase in cell abundance compared to heavily silicified diatoms along wi...
Published in: | Journal of Phycology |
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crwiley:10.1111/jpy.12979 2024-05-19T07:32:08+00:00 Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 Rizkallah, Mariam R. Frickenhaus, Stephan Trimborn, Scarlett Harms, Lars Moustafa, Ahmed Benes, Vladimir Gäbler‐Schwarz, Steffi Beszteri, Sara Mock, T. Deutsche Forschungsgemeinschaft Helmholtz-Gemeinschaft 2020 http://dx.doi.org/10.1111/jpy.12979 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12979 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12979 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.12979 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Journal of Phycology volume 56, issue 3, page 747-760 ISSN 0022-3646 1529-8817 Plant Science Aquatic Science journal-article 2020 crwiley https://doi.org/10.1111/jpy.12979 2024-04-22T07:32:37Z The haptophyte Phaeocystis antarctica is endemic to the Southern Ocean, where iron supply is sporadic and its availability limits primary production. In iron fertilization experiments, P. antarctica showed a prompt and steady increase in cell abundance compared to heavily silicified diatoms along with enhanced colony formation. Here we utilized a transcriptomic approach to investigate molecular responses to alleviation of iron limitation in P. antarctica . We analyzed the transcriptomic response before and after (14 h, 24 h and 72 h) iron addition to a low‐iron acclimated culture. After iron addition, we observed indicators of a quick reorganization of cellular energetics, from carbohydrate catabolism and mitochondrial energy production to anabolism. In addition to typical substitution responses from an iron‐economic toward an iron‐sufficient state for flavodoxin (ferredoxin) and plastocyanin (cytochrome c 6 ), we found other genes utilizing the same strategy involved in nitrogen assimilation and fatty acid desaturation. Our results shed light on a number of adaptive mechanisms that P. antarctica uses under low iron, including the utilization of a Cu‐dependent ferric reductase system and indication of mixotrophic growth. The gene expression patterns underpin P. antarctica as a quick responder to iron addition. Article in Journal/Newspaper Antarc* Antarctica Southern Ocean Wiley Online Library Journal of Phycology 56 3 747 760 |
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English |
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Plant Science Aquatic Science |
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Plant Science Aquatic Science Rizkallah, Mariam R. Frickenhaus, Stephan Trimborn, Scarlett Harms, Lars Moustafa, Ahmed Benes, Vladimir Gäbler‐Schwarz, Steffi Beszteri, Sara Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
topic_facet |
Plant Science Aquatic Science |
description |
The haptophyte Phaeocystis antarctica is endemic to the Southern Ocean, where iron supply is sporadic and its availability limits primary production. In iron fertilization experiments, P. antarctica showed a prompt and steady increase in cell abundance compared to heavily silicified diatoms along with enhanced colony formation. Here we utilized a transcriptomic approach to investigate molecular responses to alleviation of iron limitation in P. antarctica . We analyzed the transcriptomic response before and after (14 h, 24 h and 72 h) iron addition to a low‐iron acclimated culture. After iron addition, we observed indicators of a quick reorganization of cellular energetics, from carbohydrate catabolism and mitochondrial energy production to anabolism. In addition to typical substitution responses from an iron‐economic toward an iron‐sufficient state for flavodoxin (ferredoxin) and plastocyanin (cytochrome c 6 ), we found other genes utilizing the same strategy involved in nitrogen assimilation and fatty acid desaturation. Our results shed light on a number of adaptive mechanisms that P. antarctica uses under low iron, including the utilization of a Cu‐dependent ferric reductase system and indication of mixotrophic growth. The gene expression patterns underpin P. antarctica as a quick responder to iron addition. |
author2 |
Mock, T. Deutsche Forschungsgemeinschaft Helmholtz-Gemeinschaft |
format |
Article in Journal/Newspaper |
author |
Rizkallah, Mariam R. Frickenhaus, Stephan Trimborn, Scarlett Harms, Lars Moustafa, Ahmed Benes, Vladimir Gäbler‐Schwarz, Steffi Beszteri, Sara |
author_facet |
Rizkallah, Mariam R. Frickenhaus, Stephan Trimborn, Scarlett Harms, Lars Moustafa, Ahmed Benes, Vladimir Gäbler‐Schwarz, Steffi Beszteri, Sara |
author_sort |
Rizkallah, Mariam R. |
title |
Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
title_short |
Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
title_full |
Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
title_fullStr |
Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
title_full_unstemmed |
Deciphering Patterns of Adaptation and Acclimation in the Transcriptome of Phaeocystis antarctica to Changing Iron Conditions 1 |
title_sort |
deciphering patterns of adaptation and acclimation in the transcriptome of phaeocystis antarctica to changing iron conditions 1 |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/jpy.12979 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12979 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12979 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.12979 |
genre |
Antarc* Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Southern Ocean |
op_source |
Journal of Phycology volume 56, issue 3, page 747-760 ISSN 0022-3646 1529-8817 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/jpy.12979 |
container_title |
Journal of Phycology |
container_volume |
56 |
container_issue |
3 |
container_start_page |
747 |
op_container_end_page |
760 |
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1799470103836753920 |