Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms
We performed a comparison of molecular and morphological diversity in a freshwater colonial genus Synura (Chrysophyceae, Stramenopiles), using the island of Newfoundland (Canada) as a case study. We examined the morphological species diversity in collections from 79 localities, and compared these fi...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jpy.12978 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12978 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12978 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.12978 |
id |
crwiley:10.1111/jpy.12978 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/jpy.12978 2024-03-24T09:03:31+00:00 Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms Škaloud, Pavel Škaloudová, Magda Jadrná, Iva Bestová, Helena Pusztai, Martin Kapustin, Dmitry Siver, Peter A. Mock, T. Grantová Agentura České Republiky 2020 http://dx.doi.org/10.1111/jpy.12978 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12978 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12978 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.12978 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 56, issue 3, page 574-591 ISSN 0022-3646 1529-8817 Plant Science Aquatic Science journal-article 2020 crwiley https://doi.org/10.1111/jpy.12978 2024-02-28T02:13:18Z We performed a comparison of molecular and morphological diversity in a freshwater colonial genus Synura (Chrysophyceae, Stramenopiles), using the island of Newfoundland (Canada) as a case study. We examined the morphological species diversity in collections from 79 localities, and compared these findings to diversity based on molecular characters for 150 strains isolated from the same sites. Of 27 species or species‐level lineages identified, only one third was recorded by both molecular and morphological techniques, showing both approaches are complementary in estimating species diversity within this genus. Eight taxa, each representing young evolutionary lineages, were recovered only by sequencing of isolated colonies, whereas ten species were recovered only microscopically. Our complex investigation, involving both morphological and molecular examinations, indicates that our knowledge of Synura diversity is still poor, limited only to a few well‐studied areas. We revealed considerable cryptic diversity within the core S. petersenii and S. leptorrhabda lineages. We further resolved the phylogenetic position of two previously described taxa, S. kristiansenii and S. petersenii f. praefracta , propose species‐level status for S. petersenii f. praefracta , and describe three new species, S. vinlandica , S. fluviatilis, and S. cornuta . Our findings add to the growing body of literature detailing distribution patterns observed in the genus, ranging from cosmopolitan species, to highly restricted taxa, to species such as S. hibernica found along coastal regions on multiple continents. Finally, our study illustrates the usefulness of combining detailed morphological information with gene sequence data to examine species diversity within chrysophyte algae. Article in Journal/Newspaper Newfoundland Wiley Online Library Canada Journal of Phycology 56 3 574 591 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Plant Science Aquatic Science |
spellingShingle |
Plant Science Aquatic Science Škaloud, Pavel Škaloudová, Magda Jadrná, Iva Bestová, Helena Pusztai, Martin Kapustin, Dmitry Siver, Peter A. Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
topic_facet |
Plant Science Aquatic Science |
description |
We performed a comparison of molecular and morphological diversity in a freshwater colonial genus Synura (Chrysophyceae, Stramenopiles), using the island of Newfoundland (Canada) as a case study. We examined the morphological species diversity in collections from 79 localities, and compared these findings to diversity based on molecular characters for 150 strains isolated from the same sites. Of 27 species or species‐level lineages identified, only one third was recorded by both molecular and morphological techniques, showing both approaches are complementary in estimating species diversity within this genus. Eight taxa, each representing young evolutionary lineages, were recovered only by sequencing of isolated colonies, whereas ten species were recovered only microscopically. Our complex investigation, involving both morphological and molecular examinations, indicates that our knowledge of Synura diversity is still poor, limited only to a few well‐studied areas. We revealed considerable cryptic diversity within the core S. petersenii and S. leptorrhabda lineages. We further resolved the phylogenetic position of two previously described taxa, S. kristiansenii and S. petersenii f. praefracta , propose species‐level status for S. petersenii f. praefracta , and describe three new species, S. vinlandica , S. fluviatilis, and S. cornuta . Our findings add to the growing body of literature detailing distribution patterns observed in the genus, ranging from cosmopolitan species, to highly restricted taxa, to species such as S. hibernica found along coastal regions on multiple continents. Finally, our study illustrates the usefulness of combining detailed morphological information with gene sequence data to examine species diversity within chrysophyte algae. |
author2 |
Mock, T. Grantová Agentura České Republiky |
format |
Article in Journal/Newspaper |
author |
Škaloud, Pavel Škaloudová, Magda Jadrná, Iva Bestová, Helena Pusztai, Martin Kapustin, Dmitry Siver, Peter A. |
author_facet |
Škaloud, Pavel Škaloudová, Magda Jadrná, Iva Bestová, Helena Pusztai, Martin Kapustin, Dmitry Siver, Peter A. |
author_sort |
Škaloud, Pavel |
title |
Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
title_short |
Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
title_full |
Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
title_fullStr |
Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
title_full_unstemmed |
Comparing Morphological and Molecular Estimates of Species Diversity in the Freshwater Genus Synura (Stramenopiles): A Model for Understanding Diversity of Eukaryotic Microorganisms |
title_sort |
comparing morphological and molecular estimates of species diversity in the freshwater genus synura (stramenopiles): a model for understanding diversity of eukaryotic microorganisms |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/jpy.12978 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12978 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12978 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpy.12978 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Journal of Phycology volume 56, issue 3, page 574-591 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.12978 |
container_title |
Journal of Phycology |
container_volume |
56 |
container_issue |
3 |
container_start_page |
574 |
op_container_end_page |
591 |
_version_ |
1794404425569140736 |