Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex
Seventy‐five diatom strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy ( SEM and TEM ), as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the ge...
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crwiley:10.1111/jpy.12489 2024-09-15T17:48:31+00:00 Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Mélanie Marie, Dominique Vaulot, Daniel Sarno, Diana Wood, M. Agence Nationale de la Recherche 2016 http://dx.doi.org/10.1111/jpy.12489 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12489 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12489 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 53, issue 1, page 161-187 ISSN 0022-3646 1529-8817 journal-article 2016 crwiley https://doi.org/10.1111/jpy.12489 2024-06-27T04:22:51Z Seventy‐five diatom strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy ( SEM and TEM ), as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus , Attheya , Chaetoceros , Cylindrotheca , Eucampia , Nitzschia , Porosira , Pseudo‐nitzschia , Shionodiscus , Thalassiosira , and Synedropsis . Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA , ITS ‐1 and ITS ‐2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi‐compensatory base changes ( HCBC s) in paired positions of the ITS ‐2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co‐occur in summer phytoplankton communities in the Beaufort Sea. C. neogracilis generally occurred as single cells but also formed short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis , but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region. Article in Journal/Newspaper Antarc* Antarctic Beaufort Sea Phytoplankton Wiley Online Library Journal of Phycology 53 1 161 187 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Seventy‐five diatom strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy ( SEM and TEM ), as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus , Attheya , Chaetoceros , Cylindrotheca , Eucampia , Nitzschia , Porosira , Pseudo‐nitzschia , Shionodiscus , Thalassiosira , and Synedropsis . Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA , ITS ‐1 and ITS ‐2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi‐compensatory base changes ( HCBC s) in paired positions of the ITS ‐2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co‐occur in summer phytoplankton communities in the Beaufort Sea. C. neogracilis generally occurred as single cells but also formed short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis , but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region. |
author2 |
Wood, M. Agence Nationale de la Recherche |
format |
Article in Journal/Newspaper |
author |
Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Mélanie Marie, Dominique Vaulot, Daniel Sarno, Diana |
spellingShingle |
Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Mélanie Marie, Dominique Vaulot, Daniel Sarno, Diana Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
author_facet |
Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Mélanie Marie, Dominique Vaulot, Daniel Sarno, Diana |
author_sort |
Balzano, Sergio |
title |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
title_short |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
title_full |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
title_fullStr |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
title_full_unstemmed |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilisspecies complex |
title_sort |
morphological and genetic diversity of beaufort sea diatoms with high contributions from the chaetoceros neogracilisspecies complex |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/jpy.12489 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12489 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12489 |
genre |
Antarc* Antarctic Beaufort Sea Phytoplankton |
genre_facet |
Antarc* Antarctic Beaufort Sea Phytoplankton |
op_source |
Journal of Phycology volume 53, issue 1, page 161-187 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.12489 |
container_title |
Journal of Phycology |
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53 |
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1 |
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161 |
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187 |
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1810289810353422336 |