Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome
Chondrus crispus Stackhouse (Gigartinales) is a red seaweed found on North Atlantic rocky shores. Electrophoresis of RNA extracts showed a prominent band with a size of around 6,000 bp. Sequencing of the band revealed several sequences with similarity to totiviruses, double‐stranded RNA viruses that...
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crwiley:10.1111/jpy.12427 2024-06-23T07:55:13+00:00 Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome Rousvoal, Sylvie Bouyer, Betty López‐Cristoffanini, Camilo Boyen, Catherine Collén, Jonas Lane, C. Centre National de Recherche Scientifique University Pierre and Marie Curie IDEALG 2016 http://dx.doi.org/10.1111/jpy.12427 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12427 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12427 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 52, issue 4, page 493-504 ISSN 0022-3646 1529-8817 journal-article 2016 crwiley https://doi.org/10.1111/jpy.12427 2024-06-13T04:20:25Z Chondrus crispus Stackhouse (Gigartinales) is a red seaweed found on North Atlantic rocky shores. Electrophoresis of RNA extracts showed a prominent band with a size of around 6,000 bp. Sequencing of the band revealed several sequences with similarity to totiviruses, double‐stranded RNA viruses that normally infect fungi. This virus‐like entity was named C. crispus virus (CcV). It should probably be regarded as an extreme viral quasispecies or a mutant swarm since low identity (<65%) was found between sequences. Totiviruses typically code for two genes: one capsid gene ( gag ) and one RNA ‐dependent RNA polymerase gene ( pol ) with a pseudoknot structure between the genes. Both the genes and the intergenic structures were found in the CcV sequences. A nonidentical gag gene was also found in the nuclear genome of C. crispus , with associated expressed sequence tags (EST) and upstream regulatory features. The gene was presumably horizontally transferred from the virus to the alga. Similar ds RNA bands were seen in extracts from different life cycle stages of C. crispus and from all geographic locations tested. In addition, similar bands were also observed in RNA extractions from other red algae; however, the significance of this apparently widespread phenomenon is unknown. Neither phenotype caused by the infection nor any virus particles or capsid proteins were identified; thus, the presence of viral particles has not been validated. These findings increase the known host range of totiviruses to include marine red algae. Article in Journal/Newspaper North Atlantic Wiley Online Library Journal of Phycology 52 4 493 504 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Chondrus crispus Stackhouse (Gigartinales) is a red seaweed found on North Atlantic rocky shores. Electrophoresis of RNA extracts showed a prominent band with a size of around 6,000 bp. Sequencing of the band revealed several sequences with similarity to totiviruses, double‐stranded RNA viruses that normally infect fungi. This virus‐like entity was named C. crispus virus (CcV). It should probably be regarded as an extreme viral quasispecies or a mutant swarm since low identity (<65%) was found between sequences. Totiviruses typically code for two genes: one capsid gene ( gag ) and one RNA ‐dependent RNA polymerase gene ( pol ) with a pseudoknot structure between the genes. Both the genes and the intergenic structures were found in the CcV sequences. A nonidentical gag gene was also found in the nuclear genome of C. crispus , with associated expressed sequence tags (EST) and upstream regulatory features. The gene was presumably horizontally transferred from the virus to the alga. Similar ds RNA bands were seen in extracts from different life cycle stages of C. crispus and from all geographic locations tested. In addition, similar bands were also observed in RNA extractions from other red algae; however, the significance of this apparently widespread phenomenon is unknown. Neither phenotype caused by the infection nor any virus particles or capsid proteins were identified; thus, the presence of viral particles has not been validated. These findings increase the known host range of totiviruses to include marine red algae. |
author2 |
Lane, C. Centre National de Recherche Scientifique University Pierre and Marie Curie IDEALG |
format |
Article in Journal/Newspaper |
author |
Rousvoal, Sylvie Bouyer, Betty López‐Cristoffanini, Camilo Boyen, Catherine Collén, Jonas |
spellingShingle |
Rousvoal, Sylvie Bouyer, Betty López‐Cristoffanini, Camilo Boyen, Catherine Collén, Jonas Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
author_facet |
Rousvoal, Sylvie Bouyer, Betty López‐Cristoffanini, Camilo Boyen, Catherine Collén, Jonas |
author_sort |
Rousvoal, Sylvie |
title |
Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
title_short |
Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
title_full |
Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
title_fullStr |
Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
title_full_unstemmed |
Mutant swarms of a totivirus‐like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome |
title_sort |
mutant swarms of a totivirus‐like entities are present in the red macroalga chondrus crispus and have been partially transferred to the nuclear genome |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/jpy.12427 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12427 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12427 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Journal of Phycology volume 52, issue 4, page 493-504 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.12427 |
container_title |
Journal of Phycology |
container_volume |
52 |
container_issue |
4 |
container_start_page |
493 |
op_container_end_page |
504 |
_version_ |
1802647699225313280 |