Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes
In an effort to better understand the diversity of genes coding for nitrogen (N) uptake and assimilation pathways among microalgae, we analyzed the transcriptomes of five phylogenetically diverse single celled algae originally isolated from the same high arctic marine region. The five photosynthetic...
Published in: | Journal of Phycology |
---|---|
Main Authors: | , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2015
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jpy.12292 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12292 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12292 |
id |
crwiley:10.1111/jpy.12292 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/jpy.12292 2024-06-23T07:50:14+00:00 Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes Terrado, Ramon Monier, Adam Edgar, Robyn Lovejoy, Connie Mock, T. Natural Science and Engineering Council (NSERC) of Canada Fonds de Recherche du Québec Genome Québec 2015 http://dx.doi.org/10.1111/jpy.12292 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12292 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12292 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 51, issue 3, page 490-506 ISSN 0022-3646 1529-8817 journal-article 2015 crwiley https://doi.org/10.1111/jpy.12292 2024-06-13T04:24:29Z In an effort to better understand the diversity of genes coding for nitrogen (N) uptake and assimilation pathways among microalgae, we analyzed the transcriptomes of five phylogenetically diverse single celled algae originally isolated from the same high arctic marine region. The five photosynthetic flagellates (a pelagophyte, dictyochophyte, chrysoph‐yte, cryptophyte and haptophyte) were grown on standard media and media with only urea or nitrate as a nitrogen source; cells were harvested during late exponential growth. Based on homolog protein sequences, transcriptomes of each alga were interrogated to retrieve genes potentially associated with nitrogen uptake and utilization pathways. We further investigated the phylogeny of poorly characterized genes and gene families that were identified. While the phylogeny of the active urea transporter ( DUR 3) was taxonomically coherent, those for the urea transporter superfamily, putative nitrilases and amidases indicated complex evolutionary histories, and preliminary evidence for horizontal gene transfers. All five algae expressed genes for ammonium assimilation and all but the chrysophyte expressed genes involved in nitrate utilization and the urea cycle. Among the four algae with nitrate transporter transcripts, we detected lower expression levels in three of these (the dictyochophyte, pelagophyte, and cryptophyte) grown in the urea only medium compared with cultures from the nitrate only media. The diversity of N pathway genes in the five algae, and their ability to grow using urea as a nitrogen source, suggest that these flagellates are able to use a variety of organic nitrogen sources, which would be an advantage in an inorganic nitrogen ‐ limited environment, such as the Arctic Ocean. Article in Journal/Newspaper Arctic Arctic Ocean Wiley Online Library Arctic Arctic Ocean Journal of Phycology 51 3 490 506 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
In an effort to better understand the diversity of genes coding for nitrogen (N) uptake and assimilation pathways among microalgae, we analyzed the transcriptomes of five phylogenetically diverse single celled algae originally isolated from the same high arctic marine region. The five photosynthetic flagellates (a pelagophyte, dictyochophyte, chrysoph‐yte, cryptophyte and haptophyte) were grown on standard media and media with only urea or nitrate as a nitrogen source; cells were harvested during late exponential growth. Based on homolog protein sequences, transcriptomes of each alga were interrogated to retrieve genes potentially associated with nitrogen uptake and utilization pathways. We further investigated the phylogeny of poorly characterized genes and gene families that were identified. While the phylogeny of the active urea transporter ( DUR 3) was taxonomically coherent, those for the urea transporter superfamily, putative nitrilases and amidases indicated complex evolutionary histories, and preliminary evidence for horizontal gene transfers. All five algae expressed genes for ammonium assimilation and all but the chrysophyte expressed genes involved in nitrate utilization and the urea cycle. Among the four algae with nitrate transporter transcripts, we detected lower expression levels in three of these (the dictyochophyte, pelagophyte, and cryptophyte) grown in the urea only medium compared with cultures from the nitrate only media. The diversity of N pathway genes in the five algae, and their ability to grow using urea as a nitrogen source, suggest that these flagellates are able to use a variety of organic nitrogen sources, which would be an advantage in an inorganic nitrogen ‐ limited environment, such as the Arctic Ocean. |
author2 |
Mock, T. Natural Science and Engineering Council (NSERC) of Canada Fonds de Recherche du Québec Genome Québec |
format |
Article in Journal/Newspaper |
author |
Terrado, Ramon Monier, Adam Edgar, Robyn Lovejoy, Connie |
spellingShingle |
Terrado, Ramon Monier, Adam Edgar, Robyn Lovejoy, Connie Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
author_facet |
Terrado, Ramon Monier, Adam Edgar, Robyn Lovejoy, Connie |
author_sort |
Terrado, Ramon |
title |
Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
title_short |
Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
title_full |
Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
title_fullStr |
Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
title_full_unstemmed |
Diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
title_sort |
diversity of nitrogen assimilation pathways among microbial photosynthetic eukaryotes |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/jpy.12292 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12292 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12292 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Journal of Phycology volume 51, issue 3, page 490-506 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.12292 |
container_title |
Journal of Phycology |
container_volume |
51 |
container_issue |
3 |
container_start_page |
490 |
op_container_end_page |
506 |
_version_ |
1802641103373991936 |