Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA
In this study, we present the first comprehensive analyses of the diversity and distribution of marine protist (micro‐, nano‐, and picoeukaryotes) in the Western Fram Strait, using 454‐pyrosequencing and high‐pressure liquid chromatography ( HPLC ) at five stations in summer 2010. Three stations (T1...
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crwiley:10.1111/jpy.12109 2024-06-23T07:52:54+00:00 Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA Kilias, Estelle Wolf, Christian Nöthig, Eva‐Maria Peeken, Ilka Metfies, Katja Mock, T. Initiative and Networking Fund 2013 http://dx.doi.org/10.1111/jpy.12109 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12109 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12109 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Phycology volume 49, issue 5, page 996-1010 ISSN 0022-3646 1529-8817 journal-article 2013 crwiley https://doi.org/10.1111/jpy.12109 2024-06-11T04:47:50Z In this study, we present the first comprehensive analyses of the diversity and distribution of marine protist (micro‐, nano‐, and picoeukaryotes) in the Western Fram Strait, using 454‐pyrosequencing and high‐pressure liquid chromatography ( HPLC ) at five stations in summer 2010. Three stations (T1; T5; T7) were influenced by Polar Water, characterized by cold water with lower salinity (<33) and different extents of ice concentrations. Atlantic Water influenced the other two stations (T6; T9). While T6 was located in the mixed water zone characterized by cold water with intermediate salinity (~33) and high ice concentrations, T9 was located in warm water with high salinity (~35) and no ice‐coverage at all. General trends in community structure according to prevailing environmental settings, observed with both methods, coincided well. At two stations, T1 and T7, characterized by lower ice concentrations, diatoms ( F ragilariopsis sp. , P orosira sp., T halassiosira spp.) dominated the protist community. The third station (T5) was ice‐covered, but has been ice‐free for ~4 weeks prior to sampling. At this station, dinoflagellates ( D inophyceae 1, W oloszynskia sp. and G yrodinium sp.) were dominant, reflecting a post‐bloom situation. At station T6 and T9, the protist communities consisted mainly of picoeukaryotes, e.g., M icromonas spp. Based on our results, 454‐pyrosequencing has proven to be an adequate tool to provide comprehensive information on the composition of protist communities. Furthermore, this study suggests that a snap‐shot of a few, but well‐chosen samples can provide an overview of community structure patterns and succession in a dynamic marine environment. Article in Journal/Newspaper Fram Strait Wiley Online Library Journal of Phycology 49 5 996 1010 |
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Open Polar |
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Wiley Online Library |
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crwiley |
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English |
description |
In this study, we present the first comprehensive analyses of the diversity and distribution of marine protist (micro‐, nano‐, and picoeukaryotes) in the Western Fram Strait, using 454‐pyrosequencing and high‐pressure liquid chromatography ( HPLC ) at five stations in summer 2010. Three stations (T1; T5; T7) were influenced by Polar Water, characterized by cold water with lower salinity (<33) and different extents of ice concentrations. Atlantic Water influenced the other two stations (T6; T9). While T6 was located in the mixed water zone characterized by cold water with intermediate salinity (~33) and high ice concentrations, T9 was located in warm water with high salinity (~35) and no ice‐coverage at all. General trends in community structure according to prevailing environmental settings, observed with both methods, coincided well. At two stations, T1 and T7, characterized by lower ice concentrations, diatoms ( F ragilariopsis sp. , P orosira sp., T halassiosira spp.) dominated the protist community. The third station (T5) was ice‐covered, but has been ice‐free for ~4 weeks prior to sampling. At this station, dinoflagellates ( D inophyceae 1, W oloszynskia sp. and G yrodinium sp.) were dominant, reflecting a post‐bloom situation. At station T6 and T9, the protist communities consisted mainly of picoeukaryotes, e.g., M icromonas spp. Based on our results, 454‐pyrosequencing has proven to be an adequate tool to provide comprehensive information on the composition of protist communities. Furthermore, this study suggests that a snap‐shot of a few, but well‐chosen samples can provide an overview of community structure patterns and succession in a dynamic marine environment. |
author2 |
Mock, T. Initiative and Networking Fund |
format |
Article in Journal/Newspaper |
author |
Kilias, Estelle Wolf, Christian Nöthig, Eva‐Maria Peeken, Ilka Metfies, Katja |
spellingShingle |
Kilias, Estelle Wolf, Christian Nöthig, Eva‐Maria Peeken, Ilka Metfies, Katja Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
author_facet |
Kilias, Estelle Wolf, Christian Nöthig, Eva‐Maria Peeken, Ilka Metfies, Katja |
author_sort |
Kilias, Estelle |
title |
Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
title_short |
Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
title_full |
Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
title_fullStr |
Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
title_full_unstemmed |
Protist distribution in the Western Fram Strait in summer 2010 based on 454‐pyrosequencing of 18S rDNA |
title_sort |
protist distribution in the western fram strait in summer 2010 based on 454‐pyrosequencing of 18s rdna |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/jpy.12109 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpy.12109 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpy.12109 |
genre |
Fram Strait |
genre_facet |
Fram Strait |
op_source |
Journal of Phycology volume 49, issue 5, page 996-1010 ISSN 0022-3646 1529-8817 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jpy.12109 |
container_title |
Journal of Phycology |
container_volume |
49 |
container_issue |
5 |
container_start_page |
996 |
op_container_end_page |
1010 |
_version_ |
1802644321415987200 |