Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels
Abstract The hormone melatonin connects environmental cues, such as photoperiod and temperature, with a number of physiological and behavioural processes, including seasonal reproduction, through binding to their cognate receptors. This study reports the structural, functional and physiological char...
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crwiley:10.1111/jpi.12590 2024-09-15T17:56:12+00:00 Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels Ciani, Elia Fontaine, Romain Maugars, Gersende Mizrahi, Naama Mayer, Ian Levavi‐Sivan, Berta Weltzien, Finn‐Arne H2020 Marie Skłodowska-Curie Actions Norges Forskningsråd 2019 http://dx.doi.org/10.1111/jpi.12590 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpi.12590 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpi.12590 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpi.12590 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Pineal Research volume 67, issue 3 ISSN 0742-3098 1600-079X journal-article 2019 crwiley https://doi.org/10.1111/jpi.12590 2024-07-23T04:13:15Z Abstract The hormone melatonin connects environmental cues, such as photoperiod and temperature, with a number of physiological and behavioural processes, including seasonal reproduction, through binding to their cognate receptors. This study reports the structural, functional and physiological characterization of five high‐affinity melatonin receptors (Mtnr1aaα, Mtnr1aaβ, Mtnr1ab, Mtnr1al, Mtnr1b) in Atlantic salmon. Phylogenetic analysis clustered salmon melatonin receptors into three monophyletic groups, Mtnr1A, Mtnr1Al and Mtnr1B, but no functional representative of the Mtnr1C group. Contrary to previous studies in vertebrates, pharmacological characterization of four receptors in COS‐7, CHO and SH‐SY5Y cell lines (Mtnr1Aaα, Mtnr1Aaβ, Mtnr1Ab, Mtnr1B) showed induction of intracellular cAMP levels following 2‐iodomelatonin or melatonin exposure. No consistent response was measured after N‐acetyl‐serotonin or serotonin exposure. Melatonin receptor genes were expressed at all levels of the hypothalamo‐pituitary‐gonad axis, with three genes ( mtnr1aaβ , mtnr1ab and mtnr1b ) detected in the pituitary. Pituitary receptors displayed daily fluctuations in mRNA levels during spring, prior to the onset of gonadal maturation, but not in autumn, strongly implying a direct involvement of melatonin in seasonal processes regulated by the pituitary. To the best of our knowledge, this is the first report of cAMP induction mediated via melatonin receptors in a teleost species. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Pineal Research 67 3 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The hormone melatonin connects environmental cues, such as photoperiod and temperature, with a number of physiological and behavioural processes, including seasonal reproduction, through binding to their cognate receptors. This study reports the structural, functional and physiological characterization of five high‐affinity melatonin receptors (Mtnr1aaα, Mtnr1aaβ, Mtnr1ab, Mtnr1al, Mtnr1b) in Atlantic salmon. Phylogenetic analysis clustered salmon melatonin receptors into three monophyletic groups, Mtnr1A, Mtnr1Al and Mtnr1B, but no functional representative of the Mtnr1C group. Contrary to previous studies in vertebrates, pharmacological characterization of four receptors in COS‐7, CHO and SH‐SY5Y cell lines (Mtnr1Aaα, Mtnr1Aaβ, Mtnr1Ab, Mtnr1B) showed induction of intracellular cAMP levels following 2‐iodomelatonin or melatonin exposure. No consistent response was measured after N‐acetyl‐serotonin or serotonin exposure. Melatonin receptor genes were expressed at all levels of the hypothalamo‐pituitary‐gonad axis, with three genes ( mtnr1aaβ , mtnr1ab and mtnr1b ) detected in the pituitary. Pituitary receptors displayed daily fluctuations in mRNA levels during spring, prior to the onset of gonadal maturation, but not in autumn, strongly implying a direct involvement of melatonin in seasonal processes regulated by the pituitary. To the best of our knowledge, this is the first report of cAMP induction mediated via melatonin receptors in a teleost species. |
author2 |
H2020 Marie Skłodowska-Curie Actions Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Ciani, Elia Fontaine, Romain Maugars, Gersende Mizrahi, Naama Mayer, Ian Levavi‐Sivan, Berta Weltzien, Finn‐Arne |
spellingShingle |
Ciani, Elia Fontaine, Romain Maugars, Gersende Mizrahi, Naama Mayer, Ian Levavi‐Sivan, Berta Weltzien, Finn‐Arne Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
author_facet |
Ciani, Elia Fontaine, Romain Maugars, Gersende Mizrahi, Naama Mayer, Ian Levavi‐Sivan, Berta Weltzien, Finn‐Arne |
author_sort |
Ciani, Elia |
title |
Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
title_short |
Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
title_full |
Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
title_fullStr |
Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
title_full_unstemmed |
Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
title_sort |
melatonin receptors in atlantic salmon stimulate camp levels in heterologous cell lines and show season‐dependent daily variations in pituitary expression levels |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/jpi.12590 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjpi.12590 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jpi.12590 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpi.12590 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Pineal Research volume 67, issue 3 ISSN 0742-3098 1600-079X |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/jpi.12590 |
container_title |
Journal of Pineal Research |
container_volume |
67 |
container_issue |
3 |
_version_ |
1810432410605584384 |